Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_2018 and EX28DRAFT_2019 overlap by 4 nucleotides EX28DRAFT_2019 and EX28DRAFT_2020 are separated by 45 nucleotides EX28DRAFT_2020 and EX28DRAFT_2021 are separated by 44 nucleotides
EX28DRAFT_2018: EX28DRAFT_2018 - 3-deoxy-D-manno-octulosonate cytidylyltransferase, at 2,028,512 to 2,029,258
_2018
EX28DRAFT_2019: EX28DRAFT_2019 - Uncharacterized conserved protein, at 2,029,255 to 2,029,437
_2019
EX28DRAFT_2020: EX28DRAFT_2020 - Uncharacterized protein conserved in bacteria, at 2,029,483 to 2,030,712
_2020
EX28DRAFT_2021: EX28DRAFT_2021 - lipid-A-disaccharide kinase (EC 2.7.1.130), at 2,030,757 to 2,031,734
_2021
Position (kb)
2029
2030
2031 Strain fitness (log2 ratio)
-1
0
1 at 2028.514 kb on - strand at 2029.469 kb on - strand at 2029.646 kb on + strand, within EX28DRAFT_2020 at 2029.772 kb on + strand, within EX28DRAFT_2020 at 2029.772 kb on + strand, within EX28DRAFT_2020 at 2029.773 kb on - strand, within EX28DRAFT_2020 at 2029.861 kb on - strand, within EX28DRAFT_2020 at 2030.316 kb on + strand, within EX28DRAFT_2020 at 2030.403 kb on + strand, within EX28DRAFT_2020 at 2030.470 kb on + strand, within EX28DRAFT_2020 at 2030.529 kb on + strand, within EX28DRAFT_2020 at 2030.543 kb on + strand, within EX28DRAFT_2020 at 2030.565 kb on + strand, within EX28DRAFT_2020 at 2030.671 kb on + strand at 2030.671 kb on + strand at 2030.672 kb on - strand at 2030.672 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 2,028,514 - -0.1 2,029,469 - +0.9 2,029,646 + EX28DRAFT_2020 0.13 +0.1 2,029,772 + EX28DRAFT_2020 0.23 +1.1 2,029,772 + EX28DRAFT_2020 0.23 +0.4 2,029,773 - EX28DRAFT_2020 0.24 +0.1 2,029,861 - EX28DRAFT_2020 0.31 +0.6 2,030,316 + EX28DRAFT_2020 0.68 +0.2 2,030,403 + EX28DRAFT_2020 0.75 +0.3 2,030,470 + EX28DRAFT_2020 0.80 -0.5 2,030,529 + EX28DRAFT_2020 0.85 -0.7 2,030,543 + EX28DRAFT_2020 0.86 +0.4 2,030,565 + EX28DRAFT_2020 0.88 +0.9 2,030,671 + +1.4 2,030,671 + -0.2 2,030,672 - +0.5 2,030,672 - -1.3
Or see this region's nucleotide sequence