Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_1888 and EX28DRAFT_1889 are separated by 764 nucleotides EX28DRAFT_1889 and EX28DRAFT_1890 are separated by 4 nucleotides EX28DRAFT_1890 and EX28DRAFT_1891 are separated by 23 nucleotides EX28DRAFT_1891 and EX28DRAFT_1892 are separated by 26 nucleotides
EX28DRAFT_1888: EX28DRAFT_1888 - Curlin associated repeat, at 1,899,584 to 1,900,039
_1888
EX28DRAFT_1889: EX28DRAFT_1889 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 1,900,804 to 1,901,454
_1889
EX28DRAFT_1890: EX28DRAFT_1890 - Curli assembly protein CsgE, at 1,901,459 to 1,901,848
_1890
EX28DRAFT_1891: EX28DRAFT_1891 - Type VIII secretion system (T8SS), CsgF protein, at 1,901,872 to 1,902,285
_1891
EX28DRAFT_1892: EX28DRAFT_1892 - Uncharacterized protein involved in formation of curli polymers, at 1,902,312 to 1,903,145
_1892
Position (kb)
1900
1901
1902 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1899.809 kb on - strand, within EX28DRAFT_1888 at 1900.065 kb on - strand at 1900.286 kb on + strand at 1900.757 kb on - strand at 1900.807 kb on + strand at 1900.808 kb on - strand at 1900.996 kb on - strand, within EX28DRAFT_1889 at 1901.128 kb on + strand, within EX28DRAFT_1889 at 1901.173 kb on - strand, within EX28DRAFT_1889 at 1901.177 kb on + strand, within EX28DRAFT_1889 at 1901.216 kb on - strand, within EX28DRAFT_1889 at 1901.216 kb on - strand, within EX28DRAFT_1889 at 1901.247 kb on + strand, within EX28DRAFT_1889 at 1901.378 kb on - strand, within EX28DRAFT_1889 at 1901.618 kb on - strand, within EX28DRAFT_1890 at 1901.618 kb on - strand, within EX28DRAFT_1890 at 1901.618 kb on - strand, within EX28DRAFT_1890 at 1901.621 kb on + strand, within EX28DRAFT_1890 at 1901.622 kb on - strand, within EX28DRAFT_1890 at 1901.622 kb on - strand, within EX28DRAFT_1890 at 1901.623 kb on + strand, within EX28DRAFT_1890 at 1901.633 kb on - strand, within EX28DRAFT_1890 at 1901.633 kb on - strand, within EX28DRAFT_1890 at 1901.634 kb on + strand, within EX28DRAFT_1890 at 1901.635 kb on - strand, within EX28DRAFT_1890 at 1901.702 kb on - strand, within EX28DRAFT_1890 at 1901.702 kb on - strand, within EX28DRAFT_1890 at 1901.702 kb on - strand, within EX28DRAFT_1890 at 1901.702 kb on - strand, within EX28DRAFT_1890 at 1901.706 kb on - strand, within EX28DRAFT_1890 at 1901.733 kb on + strand, within EX28DRAFT_1890 at 1901.799 kb on + strand, within EX28DRAFT_1890 at 1901.814 kb on + strand at 1901.815 kb on - strand at 1901.815 kb on - strand at 1901.847 kb on - strand at 1901.885 kb on - strand at 1901.885 kb on - strand at 1901.885 kb on - strand at 1901.980 kb on - strand, within EX28DRAFT_1891 at 1902.039 kb on - strand, within EX28DRAFT_1891 at 1902.130 kb on - strand, within EX28DRAFT_1891 at 1902.290 kb on + strand at 1902.294 kb on + strand at 1902.294 kb on + strand at 1902.294 kb on + strand at 1902.295 kb on - strand at 1902.295 kb on - strand at 1902.329 kb on - strand at 1902.329 kb on - strand at 1902.347 kb on - strand at 1902.361 kb on + strand at 1902.374 kb on + strand at 1902.374 kb on + strand at 1902.374 kb on + strand at 1902.392 kb on + strand at 1902.398 kb on - strand, within EX28DRAFT_1892
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 1,899,809 - EX28DRAFT_1888 0.49 -0.1 1,900,065 - -0.2 1,900,286 + -0.4 1,900,757 - -0.4 1,900,807 + +0.7 1,900,808 - -0.9 1,900,996 - EX28DRAFT_1889 0.29 -0.3 1,901,128 + EX28DRAFT_1889 0.50 +0.1 1,901,173 - EX28DRAFT_1889 0.57 -0.3 1,901,177 + EX28DRAFT_1889 0.57 -3.2 1,901,216 - EX28DRAFT_1889 0.63 -0.4 1,901,216 - EX28DRAFT_1889 0.63 -0.3 1,901,247 + EX28DRAFT_1889 0.68 -0.9 1,901,378 - EX28DRAFT_1889 0.88 -1.1 1,901,618 - EX28DRAFT_1890 0.41 +0.5 1,901,618 - EX28DRAFT_1890 0.41 +0.4 1,901,618 - EX28DRAFT_1890 0.41 -0.4 1,901,621 + EX28DRAFT_1890 0.42 -1.8 1,901,622 - EX28DRAFT_1890 0.42 -1.0 1,901,622 - EX28DRAFT_1890 0.42 -0.1 1,901,623 + EX28DRAFT_1890 0.42 -0.4 1,901,633 - EX28DRAFT_1890 0.45 +0.5 1,901,633 - EX28DRAFT_1890 0.45 -0.1 1,901,634 + EX28DRAFT_1890 0.45 -0.5 1,901,635 - EX28DRAFT_1890 0.45 -0.8 1,901,702 - EX28DRAFT_1890 0.62 +0.9 1,901,702 - EX28DRAFT_1890 0.62 +0.5 1,901,702 - EX28DRAFT_1890 0.62 -0.3 1,901,702 - EX28DRAFT_1890 0.62 -1.0 1,901,706 - EX28DRAFT_1890 0.63 -0.6 1,901,733 + EX28DRAFT_1890 0.70 -0.3 1,901,799 + EX28DRAFT_1890 0.87 +0.6 1,901,814 + -0.5 1,901,815 - -0.1 1,901,815 - +1.3 1,901,847 - +0.0 1,901,885 - -0.6 1,901,885 - +0.2 1,901,885 - -0.7 1,901,980 - EX28DRAFT_1891 0.26 -0.7 1,902,039 - EX28DRAFT_1891 0.40 -0.0 1,902,130 - EX28DRAFT_1891 0.62 -0.6 1,902,290 + +0.2 1,902,294 + -0.5 1,902,294 + +0.2 1,902,294 + -0.3 1,902,295 - -0.2 1,902,295 - +0.5 1,902,329 - +0.1 1,902,329 - +0.0 1,902,347 - +0.7 1,902,361 + +0.2 1,902,374 + +1.3 1,902,374 + +0.0 1,902,374 + +0.1 1,902,392 + +0.0 1,902,398 - EX28DRAFT_1892 0.10 -0.5
Or see this region's nucleotide sequence