Experiment: M9 with casaminos and trichloroethylene 76.3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0500 and EX28DRAFT_0501 are separated by 15 nucleotides EX28DRAFT_0501 and EX28DRAFT_0502 are separated by 24 nucleotides EX28DRAFT_0502 and EX28DRAFT_0503 are separated by 2 nucleotides EX28DRAFT_0503 and EX28DRAFT_0504 are separated by 2 nucleotides
EX28DRAFT_0500: EX28DRAFT_0500 - colanic acid biosynthesis glycosyl transferase WcaE, at 492,131 to 492,877
_0500
EX28DRAFT_0501: EX28DRAFT_0501 - colanic acid biosynthesis acetyltransferase WcaF, at 492,893 to 493,441
_0501
EX28DRAFT_0502: EX28DRAFT_0502 - GDP-mannose 4,6-dehydratase, at 493,466 to 494,587
_0502
EX28DRAFT_0503: EX28DRAFT_0503 - Nucleoside-diphosphate-sugar epimerases, at 494,590 to 495,555
_0503
EX28DRAFT_0504: EX28DRAFT_0504 - ADP-ribose pyrophosphatase, at 495,558 to 496,037
_0504
Position (kb) 493
494
495 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 492.473 kb on + strand, within EX28DRAFT_0500 at 492.474 kb on - strand, within EX28DRAFT_0500 at 492.474 kb on - strand, within EX28DRAFT_0500 at 492.542 kb on + strand, within EX28DRAFT_0500 at 492.543 kb on - strand, within EX28DRAFT_0500 at 492.544 kb on + strand, within EX28DRAFT_0500 at 492.549 kb on - strand, within EX28DRAFT_0500 at 492.629 kb on - strand, within EX28DRAFT_0500 at 493.152 kb on - strand, within EX28DRAFT_0501 at 493.385 kb on - strand, within EX28DRAFT_0501 at 493.439 kb on + strand at 493.451 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.461 kb on + strand at 493.552 kb on - strand at 493.562 kb on - strand at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.603 kb on + strand, within EX28DRAFT_0502 at 493.604 kb on - strand, within EX28DRAFT_0502 at 493.699 kb on - strand, within EX28DRAFT_0502 at 493.699 kb on - strand, within EX28DRAFT_0502 at 493.952 kb on + strand, within EX28DRAFT_0502 at 493.958 kb on + strand, within EX28DRAFT_0502 at 493.997 kb on + strand, within EX28DRAFT_0502 at 493.997 kb on + strand, within EX28DRAFT_0502 at 493.997 kb on + strand, within EX28DRAFT_0502 at 493.998 kb on - strand, within EX28DRAFT_0502 at 493.998 kb on - strand, within EX28DRAFT_0502 at 493.998 kb on - strand, within EX28DRAFT_0502 at 494.605 kb on - strand at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.889 kb on + strand, within EX28DRAFT_0503 at 494.996 kb on - strand, within EX28DRAFT_0503 at 495.049 kb on + strand, within EX28DRAFT_0503 at 495.378 kb on - strand, within EX28DRAFT_0503 at 495.511 kb on - strand at 495.514 kb on + strand at 495.514 kb on + strand at 495.515 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with casaminos and trichloroethylene 76.3 uM remove 492,473 + EX28DRAFT_0500 0.46 +2.2 492,474 - EX28DRAFT_0500 0.46 +3.4 492,474 - EX28DRAFT_0500 0.46 +1.4 492,542 + EX28DRAFT_0500 0.55 -0.2 492,543 - EX28DRAFT_0500 0.55 +0.8 492,544 + EX28DRAFT_0500 0.55 +0.1 492,549 - EX28DRAFT_0500 0.56 -0.3 492,629 - EX28DRAFT_0500 0.67 +0.3 493,152 - EX28DRAFT_0501 0.47 +0.2 493,385 - EX28DRAFT_0501 0.90 -0.7 493,439 + -0.0 493,451 + -0.6 493,461 + -0.3 493,461 + -1.5 493,461 + -0.8 493,461 + +0.1 493,461 + -0.6 493,552 - +2.4 493,562 - -0.2 493,603 + EX28DRAFT_0502 0.12 -1.3 493,603 + EX28DRAFT_0502 0.12 +0.3 493,603 + EX28DRAFT_0502 0.12 -1.0 493,603 + EX28DRAFT_0502 0.12 +0.5 493,604 - EX28DRAFT_0502 0.12 -0.1 493,699 - EX28DRAFT_0502 0.21 -0.2 493,699 - EX28DRAFT_0502 0.21 +0.2 493,952 + EX28DRAFT_0502 0.43 -0.6 493,958 + EX28DRAFT_0502 0.44 -0.6 493,997 + EX28DRAFT_0502 0.47 -1.8 493,997 + EX28DRAFT_0502 0.47 -0.2 493,997 + EX28DRAFT_0502 0.47 -0.7 493,998 - EX28DRAFT_0502 0.47 -1.2 493,998 - EX28DRAFT_0502 0.47 -0.1 493,998 - EX28DRAFT_0502 0.47 -0.4 494,605 - +0.1 494,768 - EX28DRAFT_0503 0.18 -0.7 494,768 - EX28DRAFT_0503 0.18 +0.5 494,889 + EX28DRAFT_0503 0.31 +0.0 494,996 - EX28DRAFT_0503 0.42 +0.3 495,049 + EX28DRAFT_0503 0.48 +0.0 495,378 - EX28DRAFT_0503 0.82 +0.8 495,511 - -0.4 495,514 + -0.7 495,514 + +0.4 495,515 - +0.2
Or see this region's nucleotide sequence