Experiment: MS_media with m-arsenite 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0502 and EX28DRAFT_0503 are separated by 2 nucleotides EX28DRAFT_0503 and EX28DRAFT_0504 are separated by 2 nucleotides EX28DRAFT_0504 and EX28DRAFT_0505 overlap by 4 nucleotides
EX28DRAFT_0502: EX28DRAFT_0502 - GDP-mannose 4,6-dehydratase, at 493,466 to 494,587
_0502
EX28DRAFT_0503: EX28DRAFT_0503 - Nucleoside-diphosphate-sugar epimerases, at 494,590 to 495,555
_0503
EX28DRAFT_0504: EX28DRAFT_0504 - ADP-ribose pyrophosphatase, at 495,558 to 496,037
_0504
EX28DRAFT_0505: EX28DRAFT_0505 - colanic acid biosynthesis glycosyl transferase WcaI, at 496,034 to 497,257
_0505
Position (kb)
495
496
497 Strain fitness (log2 ratio)
-1
0
1
2 at 494.605 kb on - strand at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.768 kb on - strand, within EX28DRAFT_0503 at 494.889 kb on + strand, within EX28DRAFT_0503 at 494.996 kb on - strand, within EX28DRAFT_0503 at 495.049 kb on + strand, within EX28DRAFT_0503 at 495.378 kb on - strand, within EX28DRAFT_0503 at 495.511 kb on - strand at 495.514 kb on + strand at 495.514 kb on + strand at 495.515 kb on - strand at 495.589 kb on + strand at 495.822 kb on + strand, within EX28DRAFT_0504 at 495.823 kb on - strand, within EX28DRAFT_0504 at 495.830 kb on + strand, within EX28DRAFT_0504 at 495.861 kb on + strand, within EX28DRAFT_0504 at 495.862 kb on - strand, within EX28DRAFT_0504 at 495.997 kb on - strand at 496.033 kb on + strand at 496.034 kb on - strand at 496.048 kb on - strand at 496.061 kb on + strand at 496.062 kb on - strand at 496.250 kb on + strand, within EX28DRAFT_0505 at 496.441 kb on + strand, within EX28DRAFT_0505 at 496.441 kb on + strand, within EX28DRAFT_0505 at 496.452 kb on - strand, within EX28DRAFT_0505 at 496.560 kb on - strand, within EX28DRAFT_0505 at 496.917 kb on - strand, within EX28DRAFT_0505
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with m-arsenite 1 mM remove 494,605 - -1.0 494,768 - EX28DRAFT_0503 0.18 -0.4 494,768 - EX28DRAFT_0503 0.18 +0.2 494,889 + EX28DRAFT_0503 0.31 +0.1 494,996 - EX28DRAFT_0503 0.42 +0.2 495,049 + EX28DRAFT_0503 0.48 -0.2 495,378 - EX28DRAFT_0503 0.82 +0.7 495,511 - -0.5 495,514 + +0.4 495,514 + +0.3 495,515 - +0.0 495,589 + +0.1 495,822 + EX28DRAFT_0504 0.55 -0.0 495,823 - EX28DRAFT_0504 0.55 -0.7 495,830 + EX28DRAFT_0504 0.57 +0.2 495,861 + EX28DRAFT_0504 0.63 -1.6 495,862 - EX28DRAFT_0504 0.63 +0.6 495,997 - -0.5 496,033 + +0.1 496,034 - +0.4 496,048 - +0.5 496,061 + +0.7 496,062 - -0.2 496,250 + EX28DRAFT_0505 0.18 +0.2 496,441 + EX28DRAFT_0505 0.33 -0.9 496,441 + EX28DRAFT_0505 0.33 +0.3 496,452 - EX28DRAFT_0505 0.34 +2.5 496,560 - EX28DRAFT_0505 0.43 +0.1 496,917 - EX28DRAFT_0505 0.72 +0.1
Or see this region's nucleotide sequence