Experiment: MS_media with arsenate 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt EX28DRAFT_0297 and EX28DRAFT_0298 are separated by 8 nucleotides EX28DRAFT_0298 and EX28DRAFT_0299 are separated by 48 nucleotides EX28DRAFT_0299 and EX28DRAFT_0300 are separated by 44 nucleotides EX28DRAFT_0300 and EX28DRAFT_0301 are separated by 13 nucleotides
EX28DRAFT_0297: EX28DRAFT_0297 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 259,344 to 260,060
_0297
EX28DRAFT_0298: EX28DRAFT_0298 - ABC-type histidine transport system, ATPase component, at 260,069 to 260,842
_0298
EX28DRAFT_0299: EX28DRAFT_0299 - Acetyltransferases, including N-acetylases of ribosomal proteins, at 260,891 to 261,400
_0299
EX28DRAFT_0300: EX28DRAFT_0300 - TIGR01777 family protein, at 261,445 to 262,338
_0300
EX28DRAFT_0301: EX28DRAFT_0301 - FolB domain, at 262,352 to 262,720
_0301
Position (kb)
260
261
262 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 259.919 kb on + strand, within EX28DRAFT_0297 at 259.919 kb on + strand, within EX28DRAFT_0297 at 259.920 kb on - strand, within EX28DRAFT_0297 at 259.920 kb on - strand, within EX28DRAFT_0297 at 259.920 kb on - strand, within EX28DRAFT_0297 at 259.932 kb on + strand, within EX28DRAFT_0297 at 259.933 kb on - strand, within EX28DRAFT_0297 at 259.972 kb on - strand, within EX28DRAFT_0297 at 259.975 kb on + strand, within EX28DRAFT_0297 at 259.975 kb on + strand, within EX28DRAFT_0297 at 259.976 kb on - strand, within EX28DRAFT_0297 at 259.976 kb on - strand, within EX28DRAFT_0297 at 259.986 kb on + strand, within EX28DRAFT_0297 at 259.986 kb on + strand, within EX28DRAFT_0297 at 259.986 kb on + strand, within EX28DRAFT_0297 at 259.987 kb on - strand, within EX28DRAFT_0297 at 259.987 kb on - strand, within EX28DRAFT_0297 at 259.987 kb on - strand, within EX28DRAFT_0297 at 260.010 kb on - strand at 260.036 kb on + strand at 260.038 kb on + strand at 260.039 kb on - strand at 260.086 kb on - strand at 260.173 kb on + strand, within EX28DRAFT_0298 at 260.222 kb on - strand, within EX28DRAFT_0298 at 260.273 kb on - strand, within EX28DRAFT_0298 at 260.275 kb on + strand, within EX28DRAFT_0298 at 260.472 kb on - strand, within EX28DRAFT_0298 at 260.840 kb on + strand at 260.841 kb on - strand at 261.136 kb on + strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.137 kb on - strand, within EX28DRAFT_0299 at 261.438 kb on - strand at 261.443 kb on - strand at 261.443 kb on - strand at 261.454 kb on + strand at 261.454 kb on + strand at 261.476 kb on - strand at 261.476 kb on - strand at 261.545 kb on - strand, within EX28DRAFT_0300 at 261.652 kb on - strand, within EX28DRAFT_0300 at 261.729 kb on + strand, within EX28DRAFT_0300 at 262.088 kb on - strand, within EX28DRAFT_0300 at 262.147 kb on - strand, within EX28DRAFT_0300 at 262.339 kb on - strand at 262.339 kb on - strand at 262.353 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MS_media with arsenate 5 mM remove 259,919 + EX28DRAFT_0297 0.80 +0.2 259,919 + EX28DRAFT_0297 0.80 -1.7 259,920 - EX28DRAFT_0297 0.80 +0.5 259,920 - EX28DRAFT_0297 0.80 -0.1 259,920 - EX28DRAFT_0297 0.80 +0.7 259,932 + EX28DRAFT_0297 0.82 +0.1 259,933 - EX28DRAFT_0297 0.82 +0.2 259,972 - EX28DRAFT_0297 0.88 -0.9 259,975 + EX28DRAFT_0297 0.88 -0.2 259,975 + EX28DRAFT_0297 0.88 +0.7 259,976 - EX28DRAFT_0297 0.88 +1.4 259,976 - EX28DRAFT_0297 0.88 +0.0 259,986 + EX28DRAFT_0297 0.90 +0.7 259,986 + EX28DRAFT_0297 0.90 +0.2 259,986 + EX28DRAFT_0297 0.90 -0.5 259,987 - EX28DRAFT_0297 0.90 -0.2 259,987 - EX28DRAFT_0297 0.90 +0.0 259,987 - EX28DRAFT_0297 0.90 -1.9 260,010 - -0.6 260,036 + -1.7 260,038 + -0.6 260,039 - +0.1 260,086 - +0.5 260,173 + EX28DRAFT_0298 0.13 -1.0 260,222 - EX28DRAFT_0298 0.20 -2.5 260,273 - EX28DRAFT_0298 0.26 +0.3 260,275 + EX28DRAFT_0298 0.27 -0.1 260,472 - EX28DRAFT_0298 0.52 -0.1 260,840 + +0.6 260,841 - +0.9 261,136 + EX28DRAFT_0299 0.48 +1.7 261,137 - EX28DRAFT_0299 0.48 +0.5 261,137 - EX28DRAFT_0299 0.48 +1.0 261,137 - EX28DRAFT_0299 0.48 +0.2 261,137 - EX28DRAFT_0299 0.48 +1.0 261,438 - -0.4 261,443 - -0.7 261,443 - +0.3 261,454 + -0.2 261,454 + -1.3 261,476 - -3.1 261,476 - -0.9 261,545 - EX28DRAFT_0300 0.11 -2.4 261,652 - EX28DRAFT_0300 0.23 -0.7 261,729 + EX28DRAFT_0300 0.32 -0.3 262,088 - EX28DRAFT_0300 0.72 -0.3 262,147 - EX28DRAFT_0300 0.79 -0.5 262,339 - -3.6 262,339 - +0.3 262,353 + +0.4
Or see this region's nucleotide sequence