Experiment: L-Histidine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS16050 and ABID97_RS16055 are separated by 20 nucleotides ABID97_RS16055 and ABID97_RS16060 overlap by 4 nucleotides ABID97_RS16060 and ABID97_RS16065 are separated by 24 nucleotides ABID97_RS16065 and ABID97_RS16070 overlap by 4 nucleotides
ABID97_RS16050: ABID97_RS16050 - isochorismatase family cysteine hydrolase, at 3,325,120 to 3,325,692
_RS16050
ABID97_RS16055: ABID97_RS16055 - aromatic-ring-hydroxylating dioxygenase subunit beta, at 3,325,713 to 3,326,198
_RS16055
ABID97_RS16060: ABID97_RS16060 - aromatic ring-hydroxylating dioxygenase subunit alpha, at 3,326,195 to 3,327,499
_RS16060
ABID97_RS16065: ABID97_RS16065 - PDR/VanB family oxidoreductase, at 3,327,524 to 3,328,489
_RS16065
ABID97_RS16070: ABID97_RS16070 - dienelactone hydrolase family protein, at 3,328,486 to 3,329,745
_RS16070
Position (kb)
3326
3327
3328 Strain fitness (log2 ratio)
-1
0
1
2 at 3325.257 kb on - strand, within ABID97_RS16050 at 3325.437 kb on - strand, within ABID97_RS16050 at 3325.964 kb on - strand, within ABID97_RS16055 at 3326.227 kb on - strand at 3326.227 kb on - strand at 3326.379 kb on + strand, within ABID97_RS16060 at 3326.574 kb on + strand, within ABID97_RS16060 at 3326.575 kb on - strand, within ABID97_RS16060 at 3327.273 kb on + strand, within ABID97_RS16060 at 3327.274 kb on - strand, within ABID97_RS16060 at 3327.274 kb on - strand, within ABID97_RS16060 at 3327.274 kb on - strand, within ABID97_RS16060 at 3327.384 kb on + strand at 3327.385 kb on - strand at 3327.385 kb on - strand at 3327.444 kb on + strand at 3327.687 kb on + strand, within ABID97_RS16065 at 3328.030 kb on + strand, within ABID97_RS16065 at 3328.055 kb on + strand, within ABID97_RS16065
Per-strain Table
Position Strand Gene LocusTag Fraction L-Histidine (C) remove 3,325,257 - ABID97_RS16050 0.24 -0.2 3,325,437 - ABID97_RS16050 0.55 +2.1 3,325,964 - ABID97_RS16055 0.52 -0.6 3,326,227 - +0.0 3,326,227 - -0.0 3,326,379 + ABID97_RS16060 0.14 +0.2 3,326,574 + ABID97_RS16060 0.29 -0.6 3,326,575 - ABID97_RS16060 0.29 +1.5 3,327,273 + ABID97_RS16060 0.83 -0.3 3,327,274 - ABID97_RS16060 0.83 +0.5 3,327,274 - ABID97_RS16060 0.83 +0.0 3,327,274 - ABID97_RS16060 0.83 -0.4 3,327,384 + -0.4 3,327,385 - +0.2 3,327,385 - +0.8 3,327,444 + -0.2 3,327,687 + ABID97_RS16065 0.17 -0.8 3,328,030 + ABID97_RS16065 0.52 -1.2 3,328,055 + ABID97_RS16065 0.55 +1.0
Or see this region's nucleotide sequence