Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13120 and RR42_RS13125 are separated by 224 nucleotides RR42_RS13125 and RR42_RS13130 are separated by 172 nucleotides RR42_RS13130 and RR42_RS13135 are separated by 85 nucleotides
RR42_RS13120: RR42_RS13120 - ribosome-associated GTPase EngA, at 2,826,421 to 2,827,764
_RS13120
RR42_RS13125: RR42_RS13125 - pyrrolo-quinoline quinone, at 2,827,989 to 2,829,110
_RS13125
RR42_RS13130: RR42_RS13130 - membrane protein, at 2,829,283 to 2,829,915
_RS13130
RR42_RS13135: RR42_RS13135 - histidyl-tRNA synthetase, at 2,830,001 to 2,831,389
_RS13135
Position (kb)
2827
2828
2829
2830 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2828.134 kb on - strand, within RR42_RS13125 at 2828.138 kb on - strand, within RR42_RS13125 at 2828.138 kb on - strand, within RR42_RS13125 at 2828.147 kb on - strand, within RR42_RS13125 at 2828.201 kb on - strand, within RR42_RS13125 at 2828.618 kb on - strand, within RR42_RS13125 at 2828.618 kb on - strand, within RR42_RS13125 at 2828.921 kb on - strand, within RR42_RS13125 at 2829.435 kb on - strand, within RR42_RS13130 at 2829.615 kb on - strand, within RR42_RS13130 at 2829.747 kb on - strand, within RR42_RS13130 at 2829.747 kb on - strand, within RR42_RS13130 at 2829.909 kb on - strand at 2829.944 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,828,134 - RR42_RS13125 0.13 -3.4 2,828,138 - RR42_RS13125 0.13 -2.8 2,828,138 - RR42_RS13125 0.13 -4.1 2,828,147 - RR42_RS13125 0.14 -2.8 2,828,201 - RR42_RS13125 0.19 -1.1 2,828,618 - RR42_RS13125 0.56 -2.9 2,828,618 - RR42_RS13125 0.56 -1.1 2,828,921 - RR42_RS13125 0.83 -2.6 2,829,435 - RR42_RS13130 0.24 +0.9 2,829,615 - RR42_RS13130 0.52 +1.3 2,829,747 - RR42_RS13130 0.73 +0.3 2,829,747 - RR42_RS13130 0.73 -0.0 2,829,909 - +1.3 2,829,944 - -0.1
Or see this region's nucleotide sequence