Experiment: R2A with Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS13055 and RR42_RS13060 are separated by 29 nucleotides RR42_RS13060 and RR42_RS13065 are separated by 88 nucleotides RR42_RS13065 and RR42_RS13070 are separated by 69 nucleotides RR42_RS13070 and RR42_RS13075 are separated by 458 nucleotides
RR42_RS13055: RR42_RS13055 - NAD-dependent protein deacylase, at 2,812,398 to 2,813,144
_RS13055
RR42_RS13060: RR42_RS13060 - 23S rRNA methyltransferase, at 2,813,174 to 2,813,917
_RS13060
RR42_RS13065: RR42_RS13065 - ribonuclease R, at 2,814,006 to 2,816,597
_RS13065
RR42_RS13070: RR42_RS13070 - tRNA-Leu, at 2,816,667 to 2,816,753
_RS13070
RR42_RS13075: RR42_RS13075 - Rrf2 family transcriptional regulator, at 2,817,212 to 2,817,646
_RS13075
Position (kb)
2814
2815
2816
2817 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2813.090 kb on + strand at 2813.090 kb on + strand at 2813.090 kb on + strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.091 kb on - strand at 2813.146 kb on + strand at 2813.147 kb on - strand at 2813.208 kb on + strand at 2813.209 kb on - strand at 2813.209 kb on - strand at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.367 kb on + strand, within RR42_RS13060 at 2813.368 kb on - strand, within RR42_RS13060 at 2813.464 kb on - strand, within RR42_RS13060 at 2813.831 kb on + strand, within RR42_RS13060 at 2813.831 kb on + strand, within RR42_RS13060 at 2813.831 kb on + strand, within RR42_RS13060 at 2813.832 kb on - strand, within RR42_RS13060 at 2813.835 kb on + strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.836 kb on - strand, within RR42_RS13060 at 2813.911 kb on + strand at 2813.928 kb on + strand at 2813.928 kb on + strand at 2813.928 kb on + strand at 2813.929 kb on - strand at 2813.929 kb on - strand at 2813.929 kb on - strand at 2814.541 kb on + strand, within RR42_RS13065 at 2814.542 kb on - strand, within RR42_RS13065 at 2814.602 kb on - strand, within RR42_RS13065 at 2814.631 kb on + strand, within RR42_RS13065 at 2814.632 kb on - strand, within RR42_RS13065 at 2814.847 kb on + strand, within RR42_RS13065 at 2814.848 kb on - strand, within RR42_RS13065 at 2814.854 kb on + strand, within RR42_RS13065 at 2814.931 kb on + strand, within RR42_RS13065 at 2814.931 kb on + strand, within RR42_RS13065 at 2814.932 kb on - strand, within RR42_RS13065 at 2814.932 kb on - strand, within RR42_RS13065 at 2814.940 kb on + strand, within RR42_RS13065 at 2815.020 kb on - strand, within RR42_RS13065 at 2815.172 kb on - strand, within RR42_RS13065 at 2815.315 kb on + strand, within RR42_RS13065 at 2815.316 kb on - strand, within RR42_RS13065 at 2815.473 kb on - strand, within RR42_RS13065 at 2815.474 kb on + strand, within RR42_RS13065 at 2815.475 kb on - strand, within RR42_RS13065 at 2815.475 kb on - strand, within RR42_RS13065 at 2815.475 kb on - strand, within RR42_RS13065 at 2815.475 kb on - strand, within RR42_RS13065 at 2815.475 kb on - strand, within RR42_RS13065 at 2815.651 kb on + strand, within RR42_RS13065 at 2815.652 kb on - strand, within RR42_RS13065 at 2815.708 kb on + strand, within RR42_RS13065 at 2815.708 kb on + strand, within RR42_RS13065 at 2815.709 kb on - strand, within RR42_RS13065 at 2815.709 kb on - strand, within RR42_RS13065 at 2815.781 kb on - strand, within RR42_RS13065 at 2815.940 kb on - strand, within RR42_RS13065 at 2815.940 kb on - strand, within RR42_RS13065 at 2816.023 kb on + strand, within RR42_RS13065 at 2816.180 kb on - strand, within RR42_RS13065 at 2816.581 kb on + strand at 2816.585 kb on + strand at 2816.585 kb on + strand at 2816.586 kb on - strand at 2816.636 kb on + strand at 2816.637 kb on - strand at 2816.637 kb on - strand at 2816.864 kb on + strand at 2816.880 kb on - strand at 2816.880 kb on - strand at 2817.020 kb on + strand at 2817.021 kb on - strand at 2817.121 kb on + strand at 2817.121 kb on + strand at 2817.122 kb on - strand at 2817.122 kb on - strand at 2817.247 kb on + strand at 2817.248 kb on - strand at 2817.248 kb on - strand at 2817.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Doxycycline hyclate 0.0001 mg/ml remove 2,813,090 + -0.4 2,813,090 + +0.9 2,813,090 + +0.3 2,813,091 - +1.3 2,813,091 - +2.7 2,813,091 - -0.4 2,813,091 - +1.9 2,813,091 - +1.7 2,813,091 - +0.1 2,813,146 + +0.7 2,813,147 - -0.9 2,813,208 + +0.4 2,813,209 - -0.8 2,813,209 - +0.4 2,813,367 + RR42_RS13060 0.26 -1.3 2,813,367 + RR42_RS13060 0.26 -1.3 2,813,367 + RR42_RS13060 0.26 +0.9 2,813,367 + RR42_RS13060 0.26 -0.2 2,813,367 + RR42_RS13060 0.26 -2.2 2,813,368 - RR42_RS13060 0.26 +0.6 2,813,464 - RR42_RS13060 0.39 -0.1 2,813,831 + RR42_RS13060 0.88 +1.6 2,813,831 + RR42_RS13060 0.88 -0.7 2,813,831 + RR42_RS13060 0.88 -0.5 2,813,832 - RR42_RS13060 0.88 +0.6 2,813,835 + RR42_RS13060 0.89 +0.9 2,813,836 - RR42_RS13060 0.89 +0.6 2,813,836 - RR42_RS13060 0.89 +0.7 2,813,836 - RR42_RS13060 0.89 +1.1 2,813,836 - RR42_RS13060 0.89 -1.2 2,813,911 + +0.1 2,813,928 + +1.4 2,813,928 + -0.7 2,813,928 + +0.0 2,813,929 - -0.3 2,813,929 - -0.4 2,813,929 - +0.9 2,814,541 + RR42_RS13065 0.21 +1.3 2,814,542 - RR42_RS13065 0.21 -0.4 2,814,602 - RR42_RS13065 0.23 -0.9 2,814,631 + RR42_RS13065 0.24 -0.1 2,814,632 - RR42_RS13065 0.24 -2.0 2,814,847 + RR42_RS13065 0.32 -2.3 2,814,848 - RR42_RS13065 0.32 -2.5 2,814,854 + RR42_RS13065 0.33 -0.7 2,814,931 + RR42_RS13065 0.36 -1.7 2,814,931 + RR42_RS13065 0.36 -2.3 2,814,932 - RR42_RS13065 0.36 -1.1 2,814,932 - RR42_RS13065 0.36 -1.4 2,814,940 + RR42_RS13065 0.36 -1.3 2,815,020 - RR42_RS13065 0.39 -0.8 2,815,172 - RR42_RS13065 0.45 -4.2 2,815,315 + RR42_RS13065 0.51 -1.9 2,815,316 - RR42_RS13065 0.51 -3.7 2,815,473 - RR42_RS13065 0.57 -2.4 2,815,474 + RR42_RS13065 0.57 -2.2 2,815,475 - RR42_RS13065 0.57 -1.4 2,815,475 - RR42_RS13065 0.57 -1.9 2,815,475 - RR42_RS13065 0.57 -4.3 2,815,475 - RR42_RS13065 0.57 -1.4 2,815,475 - RR42_RS13065 0.57 -3.0 2,815,651 + RR42_RS13065 0.63 -1.4 2,815,652 - RR42_RS13065 0.64 -2.8 2,815,708 + RR42_RS13065 0.66 -2.1 2,815,708 + RR42_RS13065 0.66 -2.6 2,815,709 - RR42_RS13065 0.66 -0.6 2,815,709 - RR42_RS13065 0.66 +0.3 2,815,781 - RR42_RS13065 0.68 -0.7 2,815,940 - RR42_RS13065 0.75 -3.4 2,815,940 - RR42_RS13065 0.75 -1.2 2,816,023 + RR42_RS13065 0.78 -2.1 2,816,180 - RR42_RS13065 0.84 -3.1 2,816,581 + -3.6 2,816,585 + -0.7 2,816,585 + -2.2 2,816,586 - -0.7 2,816,636 + -0.1 2,816,637 - -1.3 2,816,637 - +0.1 2,816,864 + -1.4 2,816,880 - +1.0 2,816,880 - +0.2 2,817,020 + -3.3 2,817,021 - -0.7 2,817,121 + +0.5 2,817,121 + +0.5 2,817,122 - +0.4 2,817,122 - -1.0 2,817,247 + -0.1 2,817,248 - -1.9 2,817,248 - -1.3 2,817,248 - +0.9
Or see this region's nucleotide sequence