Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07015 and MMJJ_RS07020 are separated by 3 nucleotides MMJJ_RS07020 and MMJJ_RS07025 are separated by 23 nucleotides MMJJ_RS07025 and MMJJ_RS07030 are separated by 113 nucleotides
MMJJ_RS07015: MMJJ_RS07015 - PFL family protein, at 1,287,231 to 1,288,607
_RS07015
MMJJ_RS07020: MMJJ_RS07020 - dCTP deaminase, at 1,288,611 to 1,289,231
_RS07020
MMJJ_RS07025: MMJJ_RS07025 - tRNA (cytidine(56)-2'-O)-methyltransferase, at 1,289,255 to 1,289,794
_RS07025
MMJJ_RS07030: MMJJ_RS07030 - hypothetical protein, at 1,289,908 to 1,292,466
_RS07030
Position (kb)
1288
1289
1290 Strain fitness (log2 ratio)
-1
0
1
2 at 1288.106 kb on + strand, within MMJJ_RS07015 at 1288.152 kb on - strand, within MMJJ_RS07015 at 1288.230 kb on - strand, within MMJJ_RS07015 at 1289.433 kb on - strand, within MMJJ_RS07025 at 1289.541 kb on - strand, within MMJJ_RS07025 at 1289.549 kb on + strand, within MMJJ_RS07025 at 1289.549 kb on + strand, within MMJJ_RS07025 at 1289.555 kb on - strand, within MMJJ_RS07025 at 1289.555 kb on - strand, within MMJJ_RS07025 at 1289.555 kb on - strand, within MMJJ_RS07025 at 1289.556 kb on + strand, within MMJJ_RS07025 at 1289.556 kb on + strand, within MMJJ_RS07025 at 1289.556 kb on + strand, within MMJJ_RS07025 at 1289.558 kb on - strand, within MMJJ_RS07025 at 1289.561 kb on - strand, within MMJJ_RS07025 at 1289.570 kb on + strand, within MMJJ_RS07025 at 1289.687 kb on + strand, within MMJJ_RS07025 at 1289.693 kb on - strand, within MMJJ_RS07025 at 1289.724 kb on + strand, within MMJJ_RS07025 at 1289.794 kb on - strand at 1289.875 kb on - strand at 1289.876 kb on + strand at 1289.888 kb on + strand at 1289.918 kb on - strand at 1289.919 kb on + strand at 1289.919 kb on + strand at 1289.936 kb on + strand at 1289.936 kb on + strand at 1289.936 kb on + strand at 1289.942 kb on - strand at 1289.949 kb on - strand at 1289.963 kb on + strand at 1289.984 kb on + strand at 1290.048 kb on + strand at 1290.048 kb on + strand at 1290.049 kb on - strand at 1290.049 kb on - strand at 1290.049 kb on - strand at 1290.049 kb on - strand at 1290.049 kb on - strand at 1290.049 kb on - strand at 1290.049 kb on - strand at 1290.050 kb on + strand at 1290.050 kb on + strand at 1290.050 kb on + strand at 1290.051 kb on - strand at 1290.051 kb on - strand at 1290.052 kb on + strand at 1290.086 kb on - strand at 1290.089 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 1,288,106 + MMJJ_RS07015 0.64 +2.1 1,288,152 - MMJJ_RS07015 0.67 +1.0 1,288,230 - MMJJ_RS07015 0.73 -0.2 1,289,433 - MMJJ_RS07025 0.33 -0.1 1,289,541 - MMJJ_RS07025 0.53 +0.3 1,289,549 + MMJJ_RS07025 0.54 +0.1 1,289,549 + MMJJ_RS07025 0.54 -0.7 1,289,555 - MMJJ_RS07025 0.56 -0.3 1,289,555 - MMJJ_RS07025 0.56 -0.2 1,289,555 - MMJJ_RS07025 0.56 +0.4 1,289,556 + MMJJ_RS07025 0.56 +0.1 1,289,556 + MMJJ_RS07025 0.56 +0.2 1,289,556 + MMJJ_RS07025 0.56 +0.1 1,289,558 - MMJJ_RS07025 0.56 -0.5 1,289,561 - MMJJ_RS07025 0.57 +0.3 1,289,570 + MMJJ_RS07025 0.58 -0.4 1,289,687 + MMJJ_RS07025 0.80 -0.2 1,289,693 - MMJJ_RS07025 0.81 +0.2 1,289,724 + MMJJ_RS07025 0.87 -0.0 1,289,794 - +0.8 1,289,875 - -0.3 1,289,876 + -0.7 1,289,888 + -0.8 1,289,918 - -0.4 1,289,919 + +0.7 1,289,919 + -0.5 1,289,936 + +0.8 1,289,936 + +1.4 1,289,936 + +1.8 1,289,942 - +1.5 1,289,949 - -1.0 1,289,963 + +2.4 1,289,984 + -0.2 1,290,048 + -0.4 1,290,048 + +1.1 1,290,049 - +1.1 1,290,049 - +0.4 1,290,049 - +1.4 1,290,049 - +1.2 1,290,049 - +1.8 1,290,049 - +1.0 1,290,049 - +1.9 1,290,050 + +1.2 1,290,050 + +0.2 1,290,050 + +0.4 1,290,051 - +0.8 1,290,051 - -0.2 1,290,052 + -1.0 1,290,086 - +0.1 1,290,089 + +0.7
Or see this region's nucleotide sequence