Experiment: Collected from freezer stock
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04125 and MMJJ_RS04130 are separated by 231 nucleotides MMJJ_RS04130 and MMJJ_RS04135 are separated by 131 nucleotides MMJJ_RS04135 and MMJJ_RS04140 are separated by 15 nucleotides
MMJJ_RS04125: MMJJ_RS04125 - tRNA (guanine(10)-N(2))-dimethyltransferase, at 764,830 to 765,951
_RS04125
MMJJ_RS04130: MMJJ_RS04130 - anaerobic ribonucleoside-triphosphate reductase, at 766,183 to 768,495
_RS04130
MMJJ_RS04135: MMJJ_RS04135 - 7-cyano-7-deazaguanine synthase QueC, at 768,627 to 769,328
_RS04135
MMJJ_RS04140: MMJJ_RS04140 - iron-containing alcohol dehydrogenase, at 769,344 to 770,348
_RS04140
Position (kb) 766
767
768
769 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 765.230 kb on - strand, within MMJJ_RS04125 at 765.231 kb on + strand, within MMJJ_RS04125 at 765.368 kb on - strand, within MMJJ_RS04125 at 765.486 kb on - strand, within MMJJ_RS04125 at 765.490 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.532 kb on - strand, within MMJJ_RS04125 at 765.533 kb on + strand, within MMJJ_RS04125 at 765.533 kb on + strand, within MMJJ_RS04125 at 765.562 kb on - strand, within MMJJ_RS04125 at 765.563 kb on + strand, within MMJJ_RS04125 at 765.571 kb on - strand, within MMJJ_RS04125 at 765.572 kb on + strand, within MMJJ_RS04125 at 765.576 kb on - strand, within MMJJ_RS04125 at 765.577 kb on + strand, within MMJJ_RS04125 at 765.580 kb on - strand, within MMJJ_RS04125 at 765.604 kb on - strand, within MMJJ_RS04125 at 765.626 kb on + strand, within MMJJ_RS04125 at 765.650 kb on + strand, within MMJJ_RS04125 at 765.691 kb on + strand, within MMJJ_RS04125 at 765.871 kb on + strand at 765.872 kb on - strand at 765.877 kb on - strand at 765.878 kb on + strand at 765.882 kb on - strand at 765.939 kb on - strand at 765.943 kb on - strand at 765.965 kb on + strand at 765.975 kb on + strand at 765.975 kb on + strand at 765.988 kb on - strand at 765.995 kb on + strand at 765.998 kb on - strand at 765.998 kb on - strand at 765.999 kb on + strand at 766.002 kb on - strand at 766.002 kb on - strand at 766.003 kb on + strand at 766.006 kb on - strand at 766.034 kb on - strand at 766.034 kb on - strand at 766.035 kb on + strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.036 kb on - strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.037 kb on + strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.038 kb on - strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.039 kb on + strand at 766.042 kb on + strand at 766.047 kb on - strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.048 kb on + strand at 766.051 kb on - strand at 766.052 kb on + strand at 766.052 kb on + strand at 766.056 kb on - strand at 766.057 kb on + strand at 766.057 kb on + strand at 766.069 kb on + strand at 766.073 kb on - strand at 766.086 kb on + strand at 766.172 kb on - strand at 766.602 kb on + strand, within MMJJ_RS04130 at 766.830 kb on + strand, within MMJJ_RS04130 at 768.471 kb on - strand at 768.472 kb on + strand at 768.504 kb on + strand at 768.519 kb on - strand at 768.558 kb on + strand at 768.558 kb on + strand at 768.558 kb on + strand at 768.563 kb on + strand at 768.589 kb on + strand at 768.590 kb on - strand at 768.590 kb on - strand at 768.591 kb on + strand at 768.602 kb on + strand at 768.606 kb on - strand at 768.607 kb on + strand at 768.607 kb on + strand at 768.611 kb on - strand at 768.636 kb on + strand at 768.636 kb on + strand at 768.643 kb on - strand at 768.644 kb on + strand at 768.644 kb on + strand at 768.671 kb on + strand at 768.717 kb on + strand, within MMJJ_RS04135 at 768.717 kb on + strand, within MMJJ_RS04135 at 768.743 kb on + strand, within MMJJ_RS04135 at 768.743 kb on + strand, within MMJJ_RS04135 at 768.755 kb on + strand, within MMJJ_RS04135 at 768.755 kb on + strand, within MMJJ_RS04135 at 768.772 kb on - strand, within MMJJ_RS04135 at 768.784 kb on - strand, within MMJJ_RS04135 at 768.785 kb on + strand, within MMJJ_RS04135 at 768.803 kb on + strand, within MMJJ_RS04135 at 769.083 kb on - strand, within MMJJ_RS04135 at 769.088 kb on - strand, within MMJJ_RS04135 at 769.094 kb on + strand, within MMJJ_RS04135 at 769.121 kb on + strand, within MMJJ_RS04135 at 769.122 kb on - strand, within MMJJ_RS04135 at 769.123 kb on + strand, within MMJJ_RS04135 at 769.198 kb on + strand, within MMJJ_RS04135 at 769.203 kb on - strand, within MMJJ_RS04135 at 769.204 kb on + strand, within MMJJ_RS04135 at 769.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Collected from freezer stock remove 765,230 - MMJJ_RS04125 0.36 -0.6 765,231 + MMJJ_RS04125 0.36 -0.3 765,368 - MMJJ_RS04125 0.48 -0.2 765,486 - MMJJ_RS04125 0.58 +0.6 765,490 - MMJJ_RS04125 0.59 +0.7 765,532 - MMJJ_RS04125 0.63 +0.1 765,532 - MMJJ_RS04125 0.63 -0.2 765,532 - MMJJ_RS04125 0.63 +0.2 765,533 + MMJJ_RS04125 0.63 -0.1 765,533 + MMJJ_RS04125 0.63 +0.2 765,562 - MMJJ_RS04125 0.65 +0.4 765,563 + MMJJ_RS04125 0.65 +0.5 765,571 - MMJJ_RS04125 0.66 -0.5 765,572 + MMJJ_RS04125 0.66 +0.2 765,576 - MMJJ_RS04125 0.66 +0.2 765,577 + MMJJ_RS04125 0.67 +1.1 765,580 - MMJJ_RS04125 0.67 +0.1 765,604 - MMJJ_RS04125 0.69 -1.3 765,626 + MMJJ_RS04125 0.71 +0.7 765,650 + MMJJ_RS04125 0.73 -0.6 765,691 + MMJJ_RS04125 0.77 -0.3 765,871 + +0.6 765,872 - -0.4 765,877 - +0.9 765,878 + -0.7 765,882 - +1.1 765,939 - -0.8 765,943 - -0.1 765,965 + +0.4 765,975 + -0.3 765,975 + -0.3 765,988 - +0.4 765,995 + +0.3 765,998 - +1.2 765,998 - +0.2 765,999 + -0.2 766,002 - +0.8 766,002 - +2.5 766,003 + -0.6 766,006 - +0.3 766,034 - +0.4 766,034 - +0.4 766,035 + +2.0 766,036 - +1.0 766,036 - -1.6 766,036 - +0.8 766,036 - +0.9 766,036 - -0.9 766,036 - +0.6 766,036 - +1.0 766,036 - +1.6 766,037 + +1.2 766,037 + +1.3 766,037 + +0.5 766,037 + +0.3 766,037 + +1.6 766,038 - +0.5 766,038 - -0.1 766,038 - -0.0 766,038 - +2.1 766,038 - +1.5 766,039 + +0.0 766,039 + +2.2 766,039 + +0.5 766,039 + +0.4 766,039 + +0.4 766,039 + -0.1 766,039 + +0.1 766,042 + -0.7 766,047 - +2.2 766,048 + -0.1 766,048 + +0.5 766,048 + +0.8 766,048 + -1.2 766,048 + +0.4 766,051 - +1.3 766,052 + +1.7 766,052 + +0.6 766,056 - +0.5 766,057 + +1.5 766,057 + +1.7 766,069 + +0.8 766,073 - -0.9 766,086 + +0.5 766,172 - +1.5 766,602 + MMJJ_RS04130 0.18 +1.2 766,830 + MMJJ_RS04130 0.28 +0.9 768,471 - +0.5 768,472 + +0.5 768,504 + -0.0 768,519 - -1.1 768,558 + -0.1 768,558 + -0.5 768,558 + +0.2 768,563 + +4.0 768,589 + +0.4 768,590 - -0.5 768,590 - -1.3 768,591 + +0.4 768,602 + -0.6 768,606 - -0.2 768,607 + -0.7 768,607 + -0.4 768,611 - -0.8 768,636 + -0.2 768,636 + +0.6 768,643 - -0.5 768,644 + -0.0 768,644 + -1.3 768,671 + -0.5 768,717 + MMJJ_RS04135 0.13 -1.7 768,717 + MMJJ_RS04135 0.13 +0.2 768,743 + MMJJ_RS04135 0.17 -1.0 768,743 + MMJJ_RS04135 0.17 -0.8 768,755 + MMJJ_RS04135 0.18 +3.2 768,755 + MMJJ_RS04135 0.18 -0.1 768,772 - MMJJ_RS04135 0.21 -1.0 768,784 - MMJJ_RS04135 0.22 -2.7 768,785 + MMJJ_RS04135 0.23 +0.6 768,803 + MMJJ_RS04135 0.25 +0.8 769,083 - MMJJ_RS04135 0.65 +3.0 769,088 - MMJJ_RS04135 0.66 +2.5 769,094 + MMJJ_RS04135 0.67 -1.3 769,121 + MMJJ_RS04135 0.70 -1.0 769,122 - MMJJ_RS04135 0.71 +0.3 769,123 + MMJJ_RS04135 0.71 +0.2 769,198 + MMJJ_RS04135 0.81 -0.7 769,203 - MMJJ_RS04135 0.82 -0.0 769,204 + MMJJ_RS04135 0.82 +0.2 769,237 - +2.2
Or see this region's nucleotide sequence