Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS15460 and RR42_RS15465 are separated by 229 nucleotides RR42_RS15465 and RR42_RS15470 are separated by 15 nucleotides RR42_RS15470 and RR42_RS15475 are separated by 42 nucleotides
RR42_RS15460: RR42_RS15460 - translocation protein TolB, at 3,340,024 to 3,341,352
_RS15460
RR42_RS15465: RR42_RS15465 - TonB-dependent receptor, at 3,341,582 to 3,342,736
_RS15465
RR42_RS15470: RR42_RS15470 - biopolymer transporter ExbD, at 3,342,752 to 3,343,189
_RS15470
RR42_RS15475: RR42_RS15475 - protein tolQ, at 3,343,232 to 3,343,915
_RS15475
Position (kb)
3341
3342
3343 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3340.679 kb on + strand, within RR42_RS15460 at 3340.679 kb on + strand, within RR42_RS15460 at 3340.680 kb on - strand, within RR42_RS15460 at 3340.680 kb on - strand, within RR42_RS15460 at 3340.811 kb on + strand, within RR42_RS15460 at 3340.812 kb on - strand, within RR42_RS15460 at 3340.956 kb on - strand, within RR42_RS15460 at 3340.956 kb on - strand, within RR42_RS15460 at 3340.992 kb on + strand, within RR42_RS15460 at 3341.375 kb on + strand at 3341.526 kb on - strand at 3341.526 kb on - strand at 3341.526 kb on - strand at 3341.535 kb on + strand at 3341.535 kb on + strand at 3341.536 kb on - strand at 3341.576 kb on + strand at 3341.577 kb on - strand at 3341.583 kb on + strand at 3342.166 kb on + strand, within RR42_RS15465 at 3342.167 kb on - strand, within RR42_RS15465 at 3342.512 kb on + strand, within RR42_RS15465 at 3342.634 kb on - strand at 3342.715 kb on - strand at 3343.122 kb on + strand, within RR42_RS15470 at 3343.122 kb on + strand, within RR42_RS15470 at 3343.464 kb on - strand, within RR42_RS15475 at 3343.486 kb on - strand, within RR42_RS15475 at 3343.486 kb on - strand, within RR42_RS15475 at 3343.684 kb on + strand, within RR42_RS15475
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 3,340,679 + RR42_RS15460 0.49 -2.2 3,340,679 + RR42_RS15460 0.49 -3.4 3,340,680 - RR42_RS15460 0.49 -2.1 3,340,680 - RR42_RS15460 0.49 -2.4 3,340,811 + RR42_RS15460 0.59 -2.1 3,340,812 - RR42_RS15460 0.59 -1.2 3,340,956 - RR42_RS15460 0.70 -1.9 3,340,956 - RR42_RS15460 0.70 -2.4 3,340,992 + RR42_RS15460 0.73 -2.4 3,341,375 + -3.1 3,341,526 - +0.2 3,341,526 - -1.1 3,341,526 - +0.5 3,341,535 + -2.1 3,341,535 + -1.3 3,341,536 - +0.6 3,341,576 + -3.1 3,341,577 - -0.1 3,341,583 + -2.7 3,342,166 + RR42_RS15465 0.51 -1.7 3,342,167 - RR42_RS15465 0.51 -0.3 3,342,512 + RR42_RS15465 0.81 -2.7 3,342,634 - -2.6 3,342,715 - -1.3 3,343,122 + RR42_RS15470 0.84 -1.3 3,343,122 + RR42_RS15470 0.84 -3.7 3,343,464 - RR42_RS15475 0.34 -1.5 3,343,486 - RR42_RS15475 0.37 -2.3 3,343,486 - RR42_RS15475 0.37 -4.1 3,343,684 + RR42_RS15475 0.66 -1.9
Or see this region's nucleotide sequence