Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS07610 and RR42_RS07615 are separated by 115 nucleotides RR42_RS07615 and RR42_RS07620 are separated by 396 nucleotides
RR42_RS07610: RR42_RS07610 - acetyl-CoA acetyltransferase, at 1,630,173 to 1,631,354
_RS07610
RR42_RS07615: RR42_RS07615 - 3-ketoacyl-ACP reductase, at 1,631,470 to 1,632,210
_RS07615
RR42_RS07620: RR42_RS07620 - polyhydroxyalkanoate synthesis repressor PhaR, at 1,632,607 to 1,633,158
_RS07620
Position (kb)
1631
1632
1633 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1630.626 kb on + strand, within RR42_RS07610 at 1630.626 kb on + strand, within RR42_RS07610 at 1630.627 kb on - strand, within RR42_RS07610 at 1630.636 kb on - strand, within RR42_RS07610 at 1630.674 kb on + strand, within RR42_RS07610 at 1630.675 kb on - strand, within RR42_RS07610 at 1631.174 kb on + strand, within RR42_RS07610 at 1631.175 kb on - strand, within RR42_RS07610 at 1631.444 kb on + strand at 1631.444 kb on + strand at 1631.451 kb on + strand at 1631.466 kb on - strand at 1631.466 kb on - strand at 1631.469 kb on + strand at 1631.469 kb on + strand at 1631.469 kb on + strand at 1631.470 kb on - strand at 1631.488 kb on + strand at 1631.488 kb on + strand at 1631.492 kb on + strand at 1631.492 kb on + strand at 1631.926 kb on + strand, within RR42_RS07615 at 1631.926 kb on + strand, within RR42_RS07615 at 1631.927 kb on - strand, within RR42_RS07615 at 1632.324 kb on + strand at 1632.401 kb on + strand at 1632.401 kb on + strand at 1632.442 kb on + strand at 1632.492 kb on + strand at 1632.492 kb on + strand at 1632.492 kb on + strand at 1632.493 kb on - strand at 1632.541 kb on + strand at 1632.541 kb on + strand at 1632.667 kb on + strand, within RR42_RS07620 at 1632.688 kb on + strand, within RR42_RS07620 at 1632.689 kb on - strand, within RR42_RS07620 at 1632.866 kb on - strand, within RR42_RS07620 at 1632.898 kb on + strand, within RR42_RS07620 at 1632.898 kb on + strand, within RR42_RS07620 at 1632.967 kb on + strand, within RR42_RS07620 at 1632.967 kb on + strand, within RR42_RS07620 at 1632.968 kb on - strand, within RR42_RS07620 at 1632.993 kb on + strand, within RR42_RS07620 at 1632.994 kb on - strand, within RR42_RS07620 at 1633.051 kb on + strand, within RR42_RS07620 at 1633.051 kb on + strand, within RR42_RS07620 at 1633.052 kb on - strand, within RR42_RS07620 at 1633.157 kb on - strand at 1633.157 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 1,630,626 + RR42_RS07610 0.38 -1.1 1,630,626 + RR42_RS07610 0.38 -0.5 1,630,627 - RR42_RS07610 0.38 -1.5 1,630,636 - RR42_RS07610 0.39 +0.7 1,630,674 + RR42_RS07610 0.42 -0.6 1,630,675 - RR42_RS07610 0.42 -2.7 1,631,174 + RR42_RS07610 0.85 +0.0 1,631,175 - RR42_RS07610 0.85 -2.5 1,631,444 + -0.5 1,631,444 + +1.3 1,631,451 + -2.3 1,631,466 - -1.6 1,631,466 - +0.8 1,631,469 + -1.2 1,631,469 + -1.7 1,631,469 + -1.2 1,631,470 - -0.3 1,631,488 + -0.9 1,631,488 + -1.2 1,631,492 + +1.3 1,631,492 + -1.8 1,631,926 + RR42_RS07615 0.62 -1.4 1,631,926 + RR42_RS07615 0.62 -0.9 1,631,927 - RR42_RS07615 0.62 -0.7 1,632,324 + -0.9 1,632,401 + +0.4 1,632,401 + +0.2 1,632,442 + +1.2 1,632,492 + +0.7 1,632,492 + -0.1 1,632,492 + -0.6 1,632,493 - +0.9 1,632,541 + -2.8 1,632,541 + -0.2 1,632,667 + RR42_RS07620 0.11 +0.6 1,632,688 + RR42_RS07620 0.15 +1.0 1,632,689 - RR42_RS07620 0.15 +1.3 1,632,866 - RR42_RS07620 0.47 +0.7 1,632,898 + RR42_RS07620 0.53 +0.7 1,632,898 + RR42_RS07620 0.53 +0.6 1,632,967 + RR42_RS07620 0.65 -0.9 1,632,967 + RR42_RS07620 0.65 -0.3 1,632,968 - RR42_RS07620 0.65 -0.1 1,632,993 + RR42_RS07620 0.70 +0.5 1,632,994 - RR42_RS07620 0.70 +0.4 1,633,051 + RR42_RS07620 0.80 +0.9 1,633,051 + RR42_RS07620 0.80 +1.1 1,633,052 - RR42_RS07620 0.81 +0.8 1,633,157 - -0.6 1,633,157 - +0.5
Or see this region's nucleotide sequence