Experiment: R2A with Vancomycin 0.5 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS01015 and RR42_RS01020 are separated by 168 nucleotides RR42_RS01020 and RR42_RS01025 overlap by 11 nucleotides RR42_RS01025 and RR42_RS01030 are separated by 253 nucleotides RR42_RS01030 and RR42_RS01035 overlap by 2 nucleotides
RR42_RS01015: RR42_RS01015 - TonB-dependent receptor, at 239,995 to 242,145
_RS01015
RR42_RS01020: RR42_RS01020 - hypothetical protein, at 242,314 to 242,781
_RS01020
RR42_RS01025: RR42_RS01025 - GTPase, at 242,771 to 244,066
_RS01025
RR42_RS01030: RR42_RS01030 - molecular chaperone DnaK, at 244,320 to 244,739
_RS01030
RR42_RS01035: RR42_RS01035 - hypothetical protein, at 244,738 to 245,046
_RS01035
Position (kb) 242
243
244
245 Strain fitness (log2 ratio) -2
-1
0
1 at 241.836 kb on - strand, within RR42_RS01015 at 242.883 kb on + strand at 242.884 kb on - strand at 243.040 kb on + strand, within RR42_RS01025 at 243.040 kb on + strand, within RR42_RS01025 at 243.041 kb on - strand, within RR42_RS01025 at 243.134 kb on + strand, within RR42_RS01025 at 243.289 kb on - strand, within RR42_RS01025 at 243.563 kb on + strand, within RR42_RS01025 at 243.564 kb on - strand, within RR42_RS01025 at 243.564 kb on - strand, within RR42_RS01025 at 243.564 kb on - strand, within RR42_RS01025 at 243.680 kb on - strand, within RR42_RS01025 at 243.786 kb on + strand, within RR42_RS01025 at 243.787 kb on - strand, within RR42_RS01025 at 244.041 kb on - strand at 244.122 kb on + strand at 244.189 kb on + strand at 244.190 kb on - strand at 244.190 kb on - strand at 244.195 kb on + strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand at 244.196 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Vancomycin 0.5 mg/ml remove 241,836 - RR42_RS01015 0.86 -0.7 242,883 + -2.2 242,884 - +1.2 243,040 + RR42_RS01025 0.21 -0.7 243,040 + RR42_RS01025 0.21 -2.2 243,041 - RR42_RS01025 0.21 +0.2 243,134 + RR42_RS01025 0.28 -0.5 243,289 - RR42_RS01025 0.40 +0.2 243,563 + RR42_RS01025 0.61 -1.5 243,564 - RR42_RS01025 0.61 -2.5 243,564 - RR42_RS01025 0.61 -1.4 243,564 - RR42_RS01025 0.61 -0.6 243,680 - RR42_RS01025 0.70 -0.7 243,786 + RR42_RS01025 0.78 +0.9 243,787 - RR42_RS01025 0.78 -2.6 244,041 - -1.2 244,122 + -1.0 244,189 + -0.4 244,190 - -0.0 244,190 - -0.7 244,195 + -0.4 244,196 - -0.5 244,196 - -0.1 244,196 - -0.9 244,196 - -1.1 244,196 - -0.1
Or see this region's nucleotide sequence