Experiment: D-Galactose 20 mM (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05340 and HMPREF1058_RS20985 are separated by 286 nucleotides HMPREF1058_RS20985 and HMPREF1058_RS05335 are separated by 294 nucleotides HMPREF1058_RS05335 and HMPREF1058_RS05330 are separated by 91 nucleotides HMPREF1058_RS05330 and HMPREF1058_RS05325 overlap by 16 nucleotides
HMPREF1058_RS05340: HMPREF1058_RS05340 - site-specific integrase, at 186,216 to 187,445
_RS05340
HMPREF1058_RS20985: HMPREF1058_RS20985 - tyrosine-type recombinase/integrase, at 187,732 to 187,899
_RS20985
HMPREF1058_RS05335: HMPREF1058_RS05335 - hypothetical protein, at 188,194 to 188,982
_RS05335
HMPREF1058_RS05330: HMPREF1058_RS05330 - helix-turn-helix domain-containing protein, at 189,074 to 189,448
_RS05330
HMPREF1058_RS05325: HMPREF1058_RS05325 - hypothetical protein, at 189,433 to 190,656
_RS05325
Position (kb) 188
189 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 187.523 kb on + strand at 187.524 kb on - strand at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.754 kb on - strand, within HMPREF1058_RS20985 at 187.850 kb on + strand, within HMPREF1058_RS20985 at 187.887 kb on - strand at 187.900 kb on + strand at 187.958 kb on + strand at 188.002 kb on + strand at 188.006 kb on + strand at 188.085 kb on - strand at 188.096 kb on - strand at 188.096 kb on - strand at 188.100 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.231 kb on - strand at 188.317 kb on + strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.344 kb on - strand, within HMPREF1058_RS05335 at 188.348 kb on + strand, within HMPREF1058_RS05335 at 188.421 kb on - strand, within HMPREF1058_RS05335 at 188.437 kb on - strand, within HMPREF1058_RS05335 at 188.489 kb on + strand, within HMPREF1058_RS05335 at 188.541 kb on + strand, within HMPREF1058_RS05335 at 188.567 kb on + strand, within HMPREF1058_RS05335 at 188.612 kb on + strand, within HMPREF1058_RS05335 at 188.612 kb on + strand, within HMPREF1058_RS05335 at 188.623 kb on - strand, within HMPREF1058_RS05335 at 188.640 kb on + strand, within HMPREF1058_RS05335 at 188.652 kb on + strand, within HMPREF1058_RS05335 at 188.721 kb on - strand, within HMPREF1058_RS05335 at 188.777 kb on + strand, within HMPREF1058_RS05335 at 188.787 kb on + strand, within HMPREF1058_RS05335 at 188.873 kb on + strand, within HMPREF1058_RS05335 at 188.874 kb on - strand, within HMPREF1058_RS05335 at 188.997 kb on + strand at 189.016 kb on + strand at 189.038 kb on - strand at 189.040 kb on + strand at 189.070 kb on + strand at 189.180 kb on + strand, within HMPREF1058_RS05330 at 189.181 kb on - strand, within HMPREF1058_RS05330 at 189.250 kb on + strand, within HMPREF1058_RS05330 at 189.264 kb on - strand, within HMPREF1058_RS05330 at 189.297 kb on - strand, within HMPREF1058_RS05330 at 189.297 kb on - strand, within HMPREF1058_RS05330 at 189.297 kb on - strand, within HMPREF1058_RS05330 at 189.390 kb on - strand, within HMPREF1058_RS05330 at 189.399 kb on + strand, within HMPREF1058_RS05330 at 189.418 kb on + strand at 189.478 kb on + strand at 189.482 kb on + strand at 189.483 kb on - strand at 189.503 kb on - strand at 189.503 kb on - strand at 189.550 kb on - strand at 189.575 kb on + strand, within HMPREF1058_RS05325 at 189.576 kb on - strand, within HMPREF1058_RS05325 at 189.603 kb on - strand, within HMPREF1058_RS05325 at 189.620 kb on - strand, within HMPREF1058_RS05325 at 189.620 kb on - strand, within HMPREF1058_RS05325 at 189.651 kb on - strand, within HMPREF1058_RS05325 at 189.705 kb on + strand, within HMPREF1058_RS05325 at 189.732 kb on + strand, within HMPREF1058_RS05325 at 189.733 kb on - strand, within HMPREF1058_RS05325 at 189.735 kb on - strand, within HMPREF1058_RS05325 at 189.745 kb on - strand, within HMPREF1058_RS05325 at 189.769 kb on + strand, within HMPREF1058_RS05325 at 189.777 kb on - strand, within HMPREF1058_RS05325 at 189.787 kb on + strand, within HMPREF1058_RS05325 at 189.834 kb on - strand, within HMPREF1058_RS05325 at 189.864 kb on + strand, within HMPREF1058_RS05325 at 189.864 kb on + strand, within HMPREF1058_RS05325 at 189.912 kb on + strand, within HMPREF1058_RS05325 at 189.972 kb on - strand, within HMPREF1058_RS05325
Per-strain Table
Position Strand Gene LocusTag Fraction D-Galactose 20 mM (C) remove 187,523 + +1.7 187,524 - +1.6 187,753 + HMPREF1058_RS20985 0.12 -2.6 187,753 + HMPREF1058_RS20985 0.12 -1.4 187,754 - HMPREF1058_RS20985 0.13 -0.7 187,850 + HMPREF1058_RS20985 0.70 +1.4 187,887 - -0.5 187,900 + -0.1 187,958 + -0.5 188,002 + -0.9 188,006 + +1.1 188,085 - +0.3 188,096 - +1.4 188,096 - -0.6 188,100 + -0.2 188,228 + -1.1 188,228 + -2.0 188,228 + -0.5 188,231 - +0.9 188,317 + HMPREF1058_RS05335 0.16 +0.6 188,318 - HMPREF1058_RS05335 0.16 +0.1 188,318 - HMPREF1058_RS05335 0.16 -0.2 188,344 - HMPREF1058_RS05335 0.19 -0.3 188,348 + HMPREF1058_RS05335 0.20 +0.0 188,421 - HMPREF1058_RS05335 0.29 -1.2 188,437 - HMPREF1058_RS05335 0.31 -0.2 188,489 + HMPREF1058_RS05335 0.37 +0.7 188,541 + HMPREF1058_RS05335 0.44 -1.0 188,567 + HMPREF1058_RS05335 0.47 -1.3 188,612 + HMPREF1058_RS05335 0.53 -0.3 188,612 + HMPREF1058_RS05335 0.53 -0.3 188,623 - HMPREF1058_RS05335 0.54 +0.5 188,640 + HMPREF1058_RS05335 0.57 -1.6 188,652 + HMPREF1058_RS05335 0.58 -2.3 188,721 - HMPREF1058_RS05335 0.67 -0.2 188,777 + HMPREF1058_RS05335 0.74 +0.8 188,787 + HMPREF1058_RS05335 0.75 +0.4 188,873 + HMPREF1058_RS05335 0.86 -0.0 188,874 - HMPREF1058_RS05335 0.86 -0.7 188,997 + -3.5 189,016 + +1.6 189,038 - +0.2 189,040 + -4.1 189,070 + -3.8 189,180 + HMPREF1058_RS05330 0.28 +0.1 189,181 - HMPREF1058_RS05330 0.29 +1.1 189,250 + HMPREF1058_RS05330 0.47 -1.7 189,264 - HMPREF1058_RS05330 0.51 -2.4 189,297 - HMPREF1058_RS05330 0.59 +0.7 189,297 - HMPREF1058_RS05330 0.59 -0.2 189,297 - HMPREF1058_RS05330 0.59 +0.3 189,390 - HMPREF1058_RS05330 0.84 -1.3 189,399 + HMPREF1058_RS05330 0.87 -2.3 189,418 + -2.6 189,478 + -0.9 189,482 + +1.8 189,483 - -0.9 189,503 - -1.5 189,503 - -1.2 189,550 - +1.0 189,575 + HMPREF1058_RS05325 0.12 -1.2 189,576 - HMPREF1058_RS05325 0.12 +1.4 189,603 - HMPREF1058_RS05325 0.14 -0.2 189,620 - HMPREF1058_RS05325 0.15 -0.5 189,620 - HMPREF1058_RS05325 0.15 +1.2 189,651 - HMPREF1058_RS05325 0.18 +0.8 189,705 + HMPREF1058_RS05325 0.22 -0.9 189,732 + HMPREF1058_RS05325 0.24 -0.8 189,733 - HMPREF1058_RS05325 0.25 +0.5 189,735 - HMPREF1058_RS05325 0.25 +0.3 189,745 - HMPREF1058_RS05325 0.25 -0.2 189,769 + HMPREF1058_RS05325 0.27 +2.2 189,777 - HMPREF1058_RS05325 0.28 +0.5 189,787 + HMPREF1058_RS05325 0.29 +0.5 189,834 - HMPREF1058_RS05325 0.33 +2.4 189,864 + HMPREF1058_RS05325 0.35 +1.8 189,864 + HMPREF1058_RS05325 0.35 +1.4 189,912 + HMPREF1058_RS05325 0.39 +0.6 189,972 - HMPREF1058_RS05325 0.44 +1.2
Or see this region's nucleotide sequence