Experiment: Neomycin 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_01065 and PS417_01070 overlap by 4 nucleotides PS417_01070 and PS417_01075 are separated by 53 nucleotides PS417_01075 and PS417_01080 overlap by 1 nucleotides
PS417_01065: PS417_01065 - ABC transporter, at 233,194 to 234,309
_01065
PS417_01070: PS417_01070 - methionine ABC transporter substrate-binding protein, at 234,306 to 235,121
_01070
PS417_01075: PS417_01075 - N5,N10-methylene tetrahydromethanopterin reductase, at 235,175 to 236,527
_01075
PS417_01080: PS417_01080 - acyl-CoA dehydrogenase, at 236,527 to 237,720
_01080
Position (kb) 235
236
237 Strain fitness (log2 ratio) -2
-1
0
1
2 at 234.388 kb on + strand, within PS417_01070 at 234.389 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.458 kb on - strand, within PS417_01070 at 234.526 kb on + strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.527 kb on - strand, within PS417_01070 at 234.646 kb on + strand, within PS417_01070 at 234.647 kb on - strand, within PS417_01070 at 234.651 kb on + strand, within PS417_01070 at 234.772 kb on + strand, within PS417_01070 at 234.772 kb on + strand, within PS417_01070 at 234.773 kb on - strand, within PS417_01070 at 234.880 kb on + strand, within PS417_01070 at 234.880 kb on + strand, within PS417_01070 at 234.957 kb on - strand, within PS417_01070 at 235.139 kb on - strand at 235.149 kb on + strand at 235.150 kb on - strand at 235.150 kb on - strand at 235.150 kb on - strand at 235.185 kb on + strand at 235.323 kb on + strand, within PS417_01075 at 235.324 kb on - strand, within PS417_01075 at 235.434 kb on + strand, within PS417_01075 at 235.434 kb on + strand, within PS417_01075 at 235.435 kb on - strand, within PS417_01075 at 235.533 kb on + strand, within PS417_01075 at 235.557 kb on + strand, within PS417_01075 at 235.621 kb on - strand, within PS417_01075 at 235.621 kb on - strand, within PS417_01075 at 235.908 kb on + strand, within PS417_01075 at 235.957 kb on - strand, within PS417_01075 at 236.020 kb on - strand, within PS417_01075 at 236.020 kb on - strand, within PS417_01075 at 236.031 kb on - strand, within PS417_01075 at 236.031 kb on - strand, within PS417_01075 at 236.031 kb on - strand, within PS417_01075 at 236.188 kb on - strand, within PS417_01075 at 236.199 kb on + strand, within PS417_01075 at 236.200 kb on - strand, within PS417_01075 at 236.428 kb on - strand at 236.428 kb on - strand at 236.568 kb on - strand at 236.570 kb on + strand at 236.570 kb on + strand at 236.571 kb on - strand at 236.588 kb on + strand at 236.589 kb on - strand at 237.031 kb on - strand, within PS417_01080 at 237.031 kb on - strand, within PS417_01080 at 237.236 kb on + strand, within PS417_01080 at 237.236 kb on + strand, within PS417_01080 at 237.236 kb on + strand, within PS417_01080 at 237.254 kb on + strand, within PS417_01080 at 237.438 kb on - strand, within PS417_01080
Per-strain Table
Position Strand Gene LocusTag Fraction Neomycin 0.04 mg/ml remove 234,388 + PS417_01070 0.10 -0.0 234,389 - PS417_01070 0.10 +0.8 234,458 - PS417_01070 0.19 +0.2 234,458 - PS417_01070 0.19 -0.0 234,458 - PS417_01070 0.19 +0.2 234,458 - PS417_01070 0.19 +0.0 234,526 + PS417_01070 0.27 +0.6 234,527 - PS417_01070 0.27 +0.2 234,527 - PS417_01070 0.27 -0.8 234,527 - PS417_01070 0.27 -0.1 234,527 - PS417_01070 0.27 +0.2 234,646 + PS417_01070 0.42 +0.0 234,647 - PS417_01070 0.42 +0.1 234,651 + PS417_01070 0.42 +0.2 234,772 + PS417_01070 0.57 -0.4 234,772 + PS417_01070 0.57 -1.2 234,773 - PS417_01070 0.57 +0.9 234,880 + PS417_01070 0.70 -0.5 234,880 + PS417_01070 0.70 +0.5 234,957 - PS417_01070 0.80 +0.5 235,139 - +0.2 235,149 + -0.0 235,150 - -1.2 235,150 - -0.5 235,150 - -1.2 235,185 + +1.1 235,323 + PS417_01075 0.11 -0.3 235,324 - PS417_01075 0.11 +0.0 235,434 + PS417_01075 0.19 -0.1 235,434 + PS417_01075 0.19 +0.0 235,435 - PS417_01075 0.19 -0.2 235,533 + PS417_01075 0.26 -0.1 235,557 + PS417_01075 0.28 +0.0 235,621 - PS417_01075 0.33 -2.5 235,621 - PS417_01075 0.33 -0.2 235,908 + PS417_01075 0.54 +1.1 235,957 - PS417_01075 0.58 +0.1 236,020 - PS417_01075 0.62 +2.1 236,020 - PS417_01075 0.62 +0.1 236,031 - PS417_01075 0.63 -0.2 236,031 - PS417_01075 0.63 -0.1 236,031 - PS417_01075 0.63 +0.5 236,188 - PS417_01075 0.75 +0.3 236,199 + PS417_01075 0.76 +0.8 236,200 - PS417_01075 0.76 +0.1 236,428 - -0.4 236,428 - -0.5 236,568 - -0.3 236,570 + +0.7 236,570 + -0.3 236,571 - -0.1 236,588 + -0.8 236,589 - -0.2 237,031 - PS417_01080 0.42 -0.0 237,031 - PS417_01080 0.42 +0.0 237,236 + PS417_01080 0.59 +1.3 237,236 + PS417_01080 0.59 +0.4 237,236 + PS417_01080 0.59 -0.4 237,254 + PS417_01080 0.61 -0.0 237,438 - PS417_01080 0.76 +0.2
Or see this region's nucleotide sequence