Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1572 and Psyr_1573 are separated by 66 nucleotides Psyr_1573 and Psyr_1574 are separated by 76 nucleotides Psyr_1574 and Psyr_1575 are separated by 132 nucleotides Psyr_1575 and Psyr_1576 are separated by 76 nucleotides
Psyr_1572: Psyr_1572 - HNH endonuclease, at 1,767,589 to 1,767,963
_1572
Psyr_1573: Psyr_1573 - cyclic nucleotide-binding protein, at 1,768,030 to 1,768,506
_1573
Psyr_1574: Psyr_1574 - oligopeptidase B, Serine peptidase, MEROPS family S09A, at 1,768,583 to 1,770,643
_1574
Psyr_1575: Psyr_1575 - conserved hypothetical protein, at 1,770,776 to 1,771,252
_1575
Psyr_1576: Psyr_1576 - Glutamine amidotransferase, class-II, at 1,771,329 to 1,772,129
_1576
Position (kb)
1768
1769
1770
1771 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1767.683 kb on + strand, within Psyr_1572 at 1767.683 kb on + strand, within Psyr_1572 at 1767.683 kb on + strand, within Psyr_1572 at 1767.684 kb on - strand, within Psyr_1572 at 1767.684 kb on - strand, within Psyr_1572 at 1767.725 kb on + strand, within Psyr_1572 at 1767.725 kb on + strand, within Psyr_1572 at 1767.726 kb on - strand, within Psyr_1572 at 1767.793 kb on + strand, within Psyr_1572 at 1767.794 kb on - strand, within Psyr_1572 at 1767.858 kb on - strand, within Psyr_1572 at 1767.858 kb on - strand, within Psyr_1572 at 1767.858 kb on - strand, within Psyr_1572 at 1767.937 kb on - strand at 1768.048 kb on + strand at 1768.048 kb on + strand at 1768.049 kb on - strand at 1768.049 kb on - strand at 1768.049 kb on - strand at 1768.049 kb on - strand at 1768.049 kb on - strand at 1768.194 kb on + strand, within Psyr_1573 at 1768.194 kb on + strand, within Psyr_1573 at 1768.195 kb on - strand, within Psyr_1573 at 1768.195 kb on - strand, within Psyr_1573 at 1768.389 kb on + strand, within Psyr_1573 at 1768.401 kb on - strand, within Psyr_1573 at 1768.414 kb on - strand, within Psyr_1573 at 1768.509 kb on + strand at 1768.509 kb on + strand at 1768.509 kb on + strand at 1768.509 kb on + strand at 1768.509 kb on + strand at 1768.509 kb on + strand at 1768.509 kb on + strand at 1768.510 kb on - strand at 1768.556 kb on + strand at 1768.559 kb on + strand at 1768.559 kb on + strand at 1768.559 kb on + strand at 1768.559 kb on + strand at 1768.560 kb on - strand at 1768.560 kb on - strand at 1768.602 kb on - strand at 1768.602 kb on - strand at 1768.612 kb on + strand at 1768.612 kb on + strand at 1768.612 kb on + strand at 1768.612 kb on + strand at 1768.735 kb on + strand at 1768.782 kb on + strand at 1768.860 kb on + strand, within Psyr_1574 at 1768.861 kb on - strand, within Psyr_1574 at 1768.861 kb on - strand, within Psyr_1574 at 1768.943 kb on + strand, within Psyr_1574 at 1768.943 kb on + strand, within Psyr_1574 at 1768.944 kb on - strand, within Psyr_1574 at 1768.944 kb on - strand, within Psyr_1574 at 1768.944 kb on - strand, within Psyr_1574 at 1768.944 kb on - strand, within Psyr_1574 at 1769.284 kb on - strand, within Psyr_1574 at 1769.382 kb on + strand, within Psyr_1574 at 1769.382 kb on + strand, within Psyr_1574 at 1769.383 kb on - strand, within Psyr_1574 at 1769.538 kb on + strand, within Psyr_1574 at 1769.538 kb on + strand, within Psyr_1574 at 1769.538 kb on + strand, within Psyr_1574 at 1769.538 kb on + strand, within Psyr_1574 at 1769.538 kb on + strand, within Psyr_1574 at 1769.538 kb on + strand, within Psyr_1574 at 1769.538 kb on + strand, within Psyr_1574 at 1769.539 kb on - strand, within Psyr_1574 at 1769.539 kb on - strand, within Psyr_1574 at 1769.539 kb on - strand, within Psyr_1574 at 1769.539 kb on - strand, within Psyr_1574 at 1769.539 kb on - strand, within Psyr_1574 at 1769.548 kb on - strand, within Psyr_1574 at 1769.597 kb on + strand, within Psyr_1574 at 1769.598 kb on - strand, within Psyr_1574 at 1769.598 kb on - strand, within Psyr_1574 at 1769.598 kb on - strand, within Psyr_1574 at 1769.751 kb on + strand, within Psyr_1574 at 1769.752 kb on - strand, within Psyr_1574 at 1769.832 kb on + strand, within Psyr_1574 at 1769.832 kb on + strand, within Psyr_1574 at 1769.833 kb on - strand, within Psyr_1574 at 1769.833 kb on - strand, within Psyr_1574 at 1769.833 kb on - strand, within Psyr_1574 at 1770.031 kb on + strand, within Psyr_1574 at 1770.175 kb on - strand, within Psyr_1574 at 1770.175 kb on - strand, within Psyr_1574 at 1770.175 kb on - strand, within Psyr_1574 at 1770.175 kb on - strand, within Psyr_1574 at 1770.184 kb on - strand, within Psyr_1574 at 1770.370 kb on - strand, within Psyr_1574 at 1770.370 kb on - strand, within Psyr_1574 at 1770.396 kb on + strand, within Psyr_1574 at 1770.397 kb on - strand, within Psyr_1574 at 1770.397 kb on - strand, within Psyr_1574 at 1770.397 kb on - strand, within Psyr_1574 at 1770.399 kb on + strand, within Psyr_1574 at 1770.399 kb on + strand, within Psyr_1574 at 1770.405 kb on + strand, within Psyr_1574 at 1770.580 kb on - strand at 1770.742 kb on + strand at 1770.743 kb on - strand at 1770.743 kb on - strand at 1770.743 kb on - strand at 1770.763 kb on + strand at 1770.844 kb on - strand, within Psyr_1575 at 1771.010 kb on - strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.040 kb on + strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.041 kb on - strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.045 kb on + strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.046 kb on - strand, within Psyr_1575 at 1771.250 kb on + strand at 1771.250 kb on + strand at 1771.250 kb on + strand at 1771.250 kb on + strand at 1771.251 kb on - strand at 1771.251 kb on - strand at 1771.339 kb on + strand at 1771.340 kb on - strand at 1771.340 kb on - strand at 1771.449 kb on + strand, within Psyr_1576 at 1771.560 kb on - strand, within Psyr_1576 at 1771.632 kb on - strand, within Psyr_1576
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 1,767,683 + Psyr_1572 0.25 -0.1 1,767,683 + Psyr_1572 0.25 +0.3 1,767,683 + Psyr_1572 0.25 -0.7 1,767,684 - Psyr_1572 0.25 -0.7 1,767,684 - Psyr_1572 0.25 -1.2 1,767,725 + Psyr_1572 0.36 -0.0 1,767,725 + Psyr_1572 0.36 +1.2 1,767,726 - Psyr_1572 0.37 -0.7 1,767,793 + Psyr_1572 0.54 -0.3 1,767,794 - Psyr_1572 0.55 -0.6 1,767,858 - Psyr_1572 0.72 +0.5 1,767,858 - Psyr_1572 0.72 +1.2 1,767,858 - Psyr_1572 0.72 -1.3 1,767,937 - -0.0 1,768,048 + -0.8 1,768,048 + +0.8 1,768,049 - +1.0 1,768,049 - -0.6 1,768,049 - +0.5 1,768,049 - +0.3 1,768,049 - +1.0 1,768,194 + Psyr_1573 0.34 -0.3 1,768,194 + Psyr_1573 0.34 -0.6 1,768,195 - Psyr_1573 0.35 -0.3 1,768,195 - Psyr_1573 0.35 -0.8 1,768,389 + Psyr_1573 0.75 -0.2 1,768,401 - Psyr_1573 0.78 -0.7 1,768,414 - Psyr_1573 0.81 -0.9 1,768,509 + +0.1 1,768,509 + +0.3 1,768,509 + -0.2 1,768,509 + +0.3 1,768,509 + +0.4 1,768,509 + +0.9 1,768,509 + -0.7 1,768,510 - +1.3 1,768,556 + -0.4 1,768,559 + +0.4 1,768,559 + -0.0 1,768,559 + -0.3 1,768,559 + -0.8 1,768,560 - +2.8 1,768,560 - +1.4 1,768,602 - +0.7 1,768,602 - -0.8 1,768,612 + +0.3 1,768,612 + +0.2 1,768,612 + +0.4 1,768,612 + +0.4 1,768,735 + -0.9 1,768,782 + +1.2 1,768,860 + Psyr_1574 0.13 +1.0 1,768,861 - Psyr_1574 0.13 +1.6 1,768,861 - Psyr_1574 0.13 -0.8 1,768,943 + Psyr_1574 0.17 +0.4 1,768,943 + Psyr_1574 0.17 +0.1 1,768,944 - Psyr_1574 0.18 +0.6 1,768,944 - Psyr_1574 0.18 -0.2 1,768,944 - Psyr_1574 0.18 +1.8 1,768,944 - Psyr_1574 0.18 +1.1 1,769,284 - Psyr_1574 0.34 +0.4 1,769,382 + Psyr_1574 0.39 +0.4 1,769,382 + Psyr_1574 0.39 -1.0 1,769,383 - Psyr_1574 0.39 -0.6 1,769,538 + Psyr_1574 0.46 -0.2 1,769,538 + Psyr_1574 0.46 +0.1 1,769,538 + Psyr_1574 0.46 +1.1 1,769,538 + Psyr_1574 0.46 -0.2 1,769,538 + Psyr_1574 0.46 +1.1 1,769,538 + Psyr_1574 0.46 +0.4 1,769,538 + Psyr_1574 0.46 -2.5 1,769,539 - Psyr_1574 0.46 -0.0 1,769,539 - Psyr_1574 0.46 -0.2 1,769,539 - Psyr_1574 0.46 +1.0 1,769,539 - Psyr_1574 0.46 -0.2 1,769,539 - Psyr_1574 0.46 -1.1 1,769,548 - Psyr_1574 0.47 -1.0 1,769,597 + Psyr_1574 0.49 +0.4 1,769,598 - Psyr_1574 0.49 +1.5 1,769,598 - Psyr_1574 0.49 +0.7 1,769,598 - Psyr_1574 0.49 +0.1 1,769,751 + Psyr_1574 0.57 -0.1 1,769,752 - Psyr_1574 0.57 -0.9 1,769,832 + Psyr_1574 0.61 -0.0 1,769,832 + Psyr_1574 0.61 +0.3 1,769,833 - Psyr_1574 0.61 +0.3 1,769,833 - Psyr_1574 0.61 -1.5 1,769,833 - Psyr_1574 0.61 +0.8 1,770,031 + Psyr_1574 0.70 +2.6 1,770,175 - Psyr_1574 0.77 -0.3 1,770,175 - Psyr_1574 0.77 -0.8 1,770,175 - Psyr_1574 0.77 -0.2 1,770,175 - Psyr_1574 0.77 +0.7 1,770,184 - Psyr_1574 0.78 +0.1 1,770,370 - Psyr_1574 0.87 -1.0 1,770,370 - Psyr_1574 0.87 -1.7 1,770,396 + Psyr_1574 0.88 +1.6 1,770,397 - Psyr_1574 0.88 +0.8 1,770,397 - Psyr_1574 0.88 -0.8 1,770,397 - Psyr_1574 0.88 -0.9 1,770,399 + Psyr_1574 0.88 -1.1 1,770,399 + Psyr_1574 0.88 -0.3 1,770,405 + Psyr_1574 0.88 -0.9 1,770,580 - +2.0 1,770,742 + +0.7 1,770,743 - +1.0 1,770,743 - -0.0 1,770,743 - +0.6 1,770,763 + -0.6 1,770,844 - Psyr_1575 0.14 +1.3 1,771,010 - Psyr_1575 0.49 -2.0 1,771,040 + Psyr_1575 0.55 +1.2 1,771,040 + Psyr_1575 0.55 -0.1 1,771,040 + Psyr_1575 0.55 -1.9 1,771,040 + Psyr_1575 0.55 -0.8 1,771,040 + Psyr_1575 0.55 +1.0 1,771,040 + Psyr_1575 0.55 +0.1 1,771,040 + Psyr_1575 0.55 +0.6 1,771,040 + Psyr_1575 0.55 -1.8 1,771,040 + Psyr_1575 0.55 -0.8 1,771,040 + Psyr_1575 0.55 +1.1 1,771,040 + Psyr_1575 0.55 -0.3 1,771,040 + Psyr_1575 0.55 -0.1 1,771,040 + Psyr_1575 0.55 +0.6 1,771,040 + Psyr_1575 0.55 +1.3 1,771,040 + Psyr_1575 0.55 +1.5 1,771,041 - Psyr_1575 0.56 -1.3 1,771,041 - Psyr_1575 0.56 +1.9 1,771,041 - Psyr_1575 0.56 +0.7 1,771,041 - Psyr_1575 0.56 -0.0 1,771,041 - Psyr_1575 0.56 -0.2 1,771,041 - Psyr_1575 0.56 +0.6 1,771,041 - Psyr_1575 0.56 +0.3 1,771,041 - Psyr_1575 0.56 +0.3 1,771,041 - Psyr_1575 0.56 +0.2 1,771,041 - Psyr_1575 0.56 +0.2 1,771,041 - Psyr_1575 0.56 +0.4 1,771,041 - Psyr_1575 0.56 -0.5 1,771,041 - Psyr_1575 0.56 -0.3 1,771,041 - Psyr_1575 0.56 +1.4 1,771,041 - Psyr_1575 0.56 -0.0 1,771,041 - Psyr_1575 0.56 +1.4 1,771,041 - Psyr_1575 0.56 -0.7 1,771,041 - Psyr_1575 0.56 +1.2 1,771,041 - Psyr_1575 0.56 -1.4 1,771,041 - Psyr_1575 0.56 -0.0 1,771,041 - Psyr_1575 0.56 +0.5 1,771,045 + Psyr_1575 0.56 +0.5 1,771,045 + Psyr_1575 0.56 +0.5 1,771,045 + Psyr_1575 0.56 +0.5 1,771,045 + Psyr_1575 0.56 +0.7 1,771,045 + Psyr_1575 0.56 +1.0 1,771,045 + Psyr_1575 0.56 -2.3 1,771,046 - Psyr_1575 0.57 -0.0 1,771,046 - Psyr_1575 0.57 -1.2 1,771,046 - Psyr_1575 0.57 -0.2 1,771,046 - Psyr_1575 0.57 +1.0 1,771,046 - Psyr_1575 0.57 -0.2 1,771,046 - Psyr_1575 0.57 -0.4 1,771,250 + -0.9 1,771,250 + +0.7 1,771,250 + -0.3 1,771,250 + +1.0 1,771,251 - -0.1 1,771,251 - +0.4 1,771,339 + -0.0 1,771,340 - +0.4 1,771,340 - +0.4 1,771,449 + Psyr_1576 0.15 +0.4 1,771,560 - Psyr_1576 0.29 +0.6 1,771,632 - Psyr_1576 0.38 -0.6
Or see this region's nucleotide sequence