Experiment: KB with Berberine 15.6 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0504 and Psyr_0505 are separated by 22 nucleotides Psyr_0505 and Psyr_0506 are separated by 270 nucleotides
Psyr_0504: Psyr_0504 - conserved domain protein, at 552,941 to 555,589
_0504
Psyr_0505: Psyr_0505 - Acyl-CoA dehydrogenase, C-terminal:Acyl-CoA dehydrogenase, central region, at 555,612 to 557,414
_0505
Psyr_0506: Psyr_0506 - L-proline dehydrogenase / delta-1-pyrroline-5-carboxylate dehydrogenase, at 557,685 to 561,587
_0506
Position (kb)
555
556
557
558 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 554.627 kb on - strand, within Psyr_0504 at 554.678 kb on + strand, within Psyr_0504 at 554.679 kb on - strand, within Psyr_0504 at 554.688 kb on + strand, within Psyr_0504 at 554.688 kb on + strand, within Psyr_0504 at 554.688 kb on + strand, within Psyr_0504 at 554.688 kb on + strand, within Psyr_0504 at 554.688 kb on + strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.689 kb on - strand, within Psyr_0504 at 554.847 kb on + strand, within Psyr_0504 at 554.847 kb on + strand, within Psyr_0504 at 554.847 kb on + strand, within Psyr_0504 at 554.847 kb on + strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.848 kb on - strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.849 kb on + strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.850 kb on - strand, within Psyr_0504 at 554.903 kb on + strand, within Psyr_0504 at 554.903 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.927 kb on + strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.928 kb on - strand, within Psyr_0504 at 554.930 kb on - strand, within Psyr_0504 at 555.039 kb on - strand, within Psyr_0504 at 555.060 kb on - strand, within Psyr_0504 at 555.060 kb on - strand, within Psyr_0504 at 555.142 kb on + strand, within Psyr_0504 at 555.173 kb on + strand, within Psyr_0504 at 555.174 kb on - strand, within Psyr_0504 at 555.359 kb on + strand at 555.359 kb on + strand at 555.359 kb on + strand at 555.360 kb on - strand at 555.360 kb on - strand at 555.509 kb on - strand at 555.509 kb on - strand at 555.841 kb on + strand, within Psyr_0505 at 555.841 kb on + strand, within Psyr_0505 at 556.121 kb on - strand, within Psyr_0505 at 556.121 kb on - strand, within Psyr_0505 at 556.150 kb on + strand, within Psyr_0505 at 556.315 kb on + strand, within Psyr_0505 at 556.315 kb on + strand, within Psyr_0505 at 556.341 kb on + strand, within Psyr_0505 at 556.342 kb on - strand, within Psyr_0505 at 556.345 kb on + strand, within Psyr_0505 at 556.345 kb on + strand, within Psyr_0505 at 556.345 kb on + strand, within Psyr_0505 at 556.345 kb on + strand, within Psyr_0505 at 556.346 kb on - strand, within Psyr_0505 at 556.346 kb on - strand, within Psyr_0505 at 556.460 kb on - strand, within Psyr_0505 at 556.478 kb on - strand, within Psyr_0505 at 556.549 kb on + strand, within Psyr_0505 at 556.549 kb on + strand, within Psyr_0505 at 556.550 kb on - strand, within Psyr_0505 at 556.550 kb on - strand, within Psyr_0505 at 556.550 kb on - strand, within Psyr_0505 at 556.631 kb on + strand, within Psyr_0505 at 556.793 kb on + strand, within Psyr_0505 at 556.830 kb on + strand, within Psyr_0505 at 556.830 kb on + strand, within Psyr_0505 at 556.830 kb on + strand, within Psyr_0505 at 556.830 kb on + strand, within Psyr_0505 at 556.831 kb on - strand, within Psyr_0505 at 556.831 kb on - strand, within Psyr_0505 at 556.831 kb on - strand, within Psyr_0505 at 556.852 kb on + strand, within Psyr_0505 at 556.856 kb on + strand, within Psyr_0505 at 556.857 kb on - strand, within Psyr_0505 at 556.857 kb on - strand, within Psyr_0505 at 557.099 kb on + strand, within Psyr_0505 at 557.099 kb on + strand, within Psyr_0505 at 557.100 kb on - strand, within Psyr_0505 at 557.100 kb on - strand, within Psyr_0505 at 557.100 kb on - strand, within Psyr_0505 at 557.159 kb on + strand, within Psyr_0505 at 557.215 kb on + strand, within Psyr_0505 at 557.261 kb on - strand at 557.261 kb on - strand at 557.368 kb on + strand at 557.369 kb on - strand at 557.369 kb on - strand at 557.369 kb on - strand at 557.369 kb on - strand at 557.423 kb on - strand at 557.423 kb on - strand at 557.423 kb on - strand at 557.449 kb on - strand at 557.573 kb on - strand at 557.772 kb on + strand at 557.772 kb on + strand at 557.772 kb on + strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.853 kb on + strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.854 kb on - strand at 557.920 kb on - strand at 558.069 kb on + strand at 558.069 kb on + strand at 558.069 kb on + strand at 558.069 kb on + strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.070 kb on - strand at 558.303 kb on - strand, within Psyr_0506 at 558.340 kb on + strand, within Psyr_0506 at 558.340 kb on + strand, within Psyr_0506 at 558.340 kb on + strand, within Psyr_0506 at 558.340 kb on + strand, within Psyr_0506 at 558.341 kb on - strand, within Psyr_0506
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 15.6 ug/ml remove 554,627 - Psyr_0504 0.64 +0.1 554,678 + Psyr_0504 0.66 -0.5 554,679 - Psyr_0504 0.66 -1.1 554,688 + Psyr_0504 0.66 +0.1 554,688 + Psyr_0504 0.66 +1.9 554,688 + Psyr_0504 0.66 +1.6 554,688 + Psyr_0504 0.66 +0.4 554,688 + Psyr_0504 0.66 +0.6 554,689 - Psyr_0504 0.66 -0.1 554,689 - Psyr_0504 0.66 +0.7 554,689 - Psyr_0504 0.66 -0.1 554,689 - Psyr_0504 0.66 -0.2 554,689 - Psyr_0504 0.66 -0.5 554,847 + Psyr_0504 0.72 -0.5 554,847 + Psyr_0504 0.72 -0.0 554,847 + Psyr_0504 0.72 -0.0 554,847 + Psyr_0504 0.72 +1.5 554,848 - Psyr_0504 0.72 +0.5 554,848 - Psyr_0504 0.72 -0.5 554,848 - Psyr_0504 0.72 -0.8 554,848 - Psyr_0504 0.72 -0.6 554,848 - Psyr_0504 0.72 +0.3 554,848 - Psyr_0504 0.72 -0.0 554,849 + Psyr_0504 0.72 -0.3 554,849 + Psyr_0504 0.72 +0.2 554,850 - Psyr_0504 0.72 +1.1 554,850 - Psyr_0504 0.72 +0.4 554,850 - Psyr_0504 0.72 -0.0 554,850 - Psyr_0504 0.72 +0.5 554,850 - Psyr_0504 0.72 -1.0 554,850 - Psyr_0504 0.72 -0.9 554,903 + Psyr_0504 0.74 +0.8 554,903 + Psyr_0504 0.74 +0.3 554,927 + Psyr_0504 0.75 -0.4 554,927 + Psyr_0504 0.75 -0.4 554,927 + Psyr_0504 0.75 -0.1 554,928 - Psyr_0504 0.75 -0.6 554,928 - Psyr_0504 0.75 -0.3 554,928 - Psyr_0504 0.75 +0.1 554,928 - Psyr_0504 0.75 -0.5 554,928 - Psyr_0504 0.75 -0.9 554,928 - Psyr_0504 0.75 -0.4 554,930 - Psyr_0504 0.75 +0.6 555,039 - Psyr_0504 0.79 +1.1 555,060 - Psyr_0504 0.80 -0.3 555,060 - Psyr_0504 0.80 -1.3 555,142 + Psyr_0504 0.83 +0.1 555,173 + Psyr_0504 0.84 -0.2 555,174 - Psyr_0504 0.84 +0.2 555,359 + -0.9 555,359 + -0.6 555,359 + +0.9 555,360 - -0.7 555,360 - +0.2 555,509 - +0.4 555,509 - -0.3 555,841 + Psyr_0505 0.13 -0.6 555,841 + Psyr_0505 0.13 -0.6 556,121 - Psyr_0505 0.28 -0.1 556,121 - Psyr_0505 0.28 -0.8 556,150 + Psyr_0505 0.30 +0.3 556,315 + Psyr_0505 0.39 -0.8 556,315 + Psyr_0505 0.39 -0.9 556,341 + Psyr_0505 0.40 -0.0 556,342 - Psyr_0505 0.40 -0.8 556,345 + Psyr_0505 0.41 +0.2 556,345 + Psyr_0505 0.41 -0.5 556,345 + Psyr_0505 0.41 +0.1 556,345 + Psyr_0505 0.41 -0.0 556,346 - Psyr_0505 0.41 +1.9 556,346 - Psyr_0505 0.41 -0.3 556,460 - Psyr_0505 0.47 +1.1 556,478 - Psyr_0505 0.48 -0.1 556,549 + Psyr_0505 0.52 +0.6 556,549 + Psyr_0505 0.52 +0.1 556,550 - Psyr_0505 0.52 -1.4 556,550 - Psyr_0505 0.52 -1.5 556,550 - Psyr_0505 0.52 +0.8 556,631 + Psyr_0505 0.57 +0.5 556,793 + Psyr_0505 0.66 -1.4 556,830 + Psyr_0505 0.68 -0.4 556,830 + Psyr_0505 0.68 +1.6 556,830 + Psyr_0505 0.68 -0.6 556,830 + Psyr_0505 0.68 +0.3 556,831 - Psyr_0505 0.68 +0.5 556,831 - Psyr_0505 0.68 +0.3 556,831 - Psyr_0505 0.68 -0.5 556,852 + Psyr_0505 0.69 -2.7 556,856 + Psyr_0505 0.69 +1.1 556,857 - Psyr_0505 0.69 +0.9 556,857 - Psyr_0505 0.69 +2.4 557,099 + Psyr_0505 0.82 +1.1 557,099 + Psyr_0505 0.82 +0.1 557,100 - Psyr_0505 0.83 +1.1 557,100 - Psyr_0505 0.83 -0.2 557,100 - Psyr_0505 0.83 -0.9 557,159 + Psyr_0505 0.86 +0.2 557,215 + Psyr_0505 0.89 -0.5 557,261 - -1.8 557,261 - -2.1 557,368 + -0.4 557,369 - +0.2 557,369 - -0.7 557,369 - +0.5 557,369 - +0.7 557,423 - +0.7 557,423 - -1.4 557,423 - +1.2 557,449 - +0.5 557,573 - +0.2 557,772 + -0.3 557,772 + -0.4 557,772 + +0.2 557,853 + -0.0 557,853 + -0.7 557,853 + -1.9 557,853 + -0.2 557,853 + +0.0 557,853 + +0.6 557,854 - -0.1 557,854 - -1.1 557,854 - +0.2 557,854 - +0.1 557,854 - -2.0 557,854 - -1.8 557,854 - -0.9 557,920 - +0.2 558,069 + -1.4 558,069 + -2.3 558,069 + +0.3 558,069 + -0.6 558,070 - +0.1 558,070 - -0.2 558,070 - -1.3 558,070 - -0.5 558,070 - +1.1 558,303 - Psyr_0506 0.16 -1.4 558,340 + Psyr_0506 0.17 -1.6 558,340 + Psyr_0506 0.17 -0.5 558,340 + Psyr_0506 0.17 -0.7 558,340 + Psyr_0506 0.17 -1.6 558,341 - Psyr_0506 0.17 -0.0
Or see this region's nucleotide sequence