Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4006 and Pf1N1B4_4007 are separated by 111 nucleotides Pf1N1B4_4007 and Pf1N1B4_4008 are separated by 367 nucleotides
Pf1N1B4_4006 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 4,455,762 to 4,457,144
_4006
Pf1N1B4_4007 - sigma-54 dependent transcriptional regulator, at 4,457,256 to 4,459,106
_4007
Pf1N1B4_4008 - Various polyols ABC transporter, ATP-binding component, at 4,459,474 to 4,460,568
_4008
Position (kb)
4457
4458
4459
4460 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4457.285 kb on - strand at 4457.285 kb on - strand at 4457.285 kb on - strand at 4457.285 kb on - strand at 4457.690 kb on - strand at 4457.690 kb on - strand, within Pf1N1B4_4007 at 4457.959 kb on - strand, within Pf1N1B4_4007 at 4457.959 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4458.605 kb on - strand, within Pf1N1B4_4007 at 4459.133 kb on - strand at 4459.213 kb on - strand at 4459.219 kb on - strand at 4459.352 kb on + strand at 4459.353 kb on - strand at 4459.459 kb on + strand at 4459.711 kb on + strand, within Pf1N1B4_4008 at 4459.712 kb on - strand, within Pf1N1B4_4008 at 4459.966 kb on + strand, within Pf1N1B4_4008 at 4460.032 kb on - strand, within Pf1N1B4_4008 at 4460.035 kb on + strand, within Pf1N1B4_4008 at 4460.036 kb on - strand, within Pf1N1B4_4008 at 4460.036 kb on - strand, within Pf1N1B4_4008 at 4460.036 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 4,457,285 - +2.2 4,457,285 - +0.2 4,457,285 - +2.8 4,457,285 - +0.3 4,457,690 - +0.5 4,457,690 - Pf1N1B4_4007 0.23 +0.3 4,457,959 - Pf1N1B4_4007 0.38 +0.9 4,457,959 - Pf1N1B4_4007 0.38 +0.4 4,458,605 - Pf1N1B4_4007 0.73 -0.2 4,458,605 - Pf1N1B4_4007 0.73 +0.3 4,458,605 - Pf1N1B4_4007 0.73 +0.6 4,458,605 - Pf1N1B4_4007 0.73 +1.3 4,459,133 - -0.0 4,459,213 - -2.1 4,459,219 - +0.3 4,459,352 + +0.9 4,459,353 - +0.5 4,459,459 + +0.5 4,459,711 + Pf1N1B4_4008 0.22 +1.9 4,459,712 - Pf1N1B4_4008 0.22 -0.3 4,459,966 + Pf1N1B4_4008 0.45 -2.2 4,460,032 - Pf1N1B4_4008 0.51 +0.8 4,460,035 + Pf1N1B4_4008 0.51 +0.9 4,460,036 - Pf1N1B4_4008 0.51 +0.4 4,460,036 - Pf1N1B4_4008 0.51 -0.5 4,460,036 - +0.9
Or see this region's nucleotide sequence