Experiment: R2A with Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS22650 and CA265_RS22655 are separated by 134 nucleotides CA265_RS22655 and CA265_RS22660 are separated by 323 nucleotides CA265_RS22660 and CA265_RS22665 are separated by 414 nucleotides
CA265_RS22650: CA265_RS22650 - branched chain amino acid aminotransferase, at 5,546,320 to 5,547,384
_RS22650
CA265_RS22655: CA265_RS22655 - hypothetical protein, at 5,547,519 to 5,547,983
_RS22655
CA265_RS22660: CA265_RS22660 - DNA repair protein RadA, at 5,548,307 to 5,549,683
_RS22660
CA265_RS22665: CA265_RS22665 - ribonuclease E/G, at 5,550,098 to 5,551,648
_RS22665
Position (kb) 5548
5549
5550 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5547.380 kb on + strand at 5547.380 kb on + strand at 5547.381 kb on - strand at 5547.382 kb on + strand at 5547.420 kb on + strand at 5547.421 kb on - strand at 5547.477 kb on + strand at 5547.520 kb on + strand at 5547.693 kb on + strand, within CA265_RS22655 at 5547.693 kb on + strand, within CA265_RS22655 at 5547.693 kb on + strand, within CA265_RS22655 at 5547.694 kb on - strand, within CA265_RS22655 at 5547.762 kb on + strand, within CA265_RS22655 at 5547.913 kb on + strand, within CA265_RS22655 at 5547.914 kb on + strand, within CA265_RS22655 at 5547.914 kb on + strand, within CA265_RS22655 at 5547.915 kb on - strand, within CA265_RS22655 at 5547.990 kb on - strand at 5548.092 kb on - strand at 5548.093 kb on + strand at 5548.305 kb on - strand at 5548.305 kb on - strand at 5548.308 kb on + strand at 5548.398 kb on + strand at 5548.398 kb on + strand at 5548.399 kb on - strand at 5548.408 kb on + strand at 5548.467 kb on - strand, within CA265_RS22660 at 5548.493 kb on + strand, within CA265_RS22660 at 5548.494 kb on - strand, within CA265_RS22660 at 5548.610 kb on - strand, within CA265_RS22660 at 5548.631 kb on - strand, within CA265_RS22660 at 5548.675 kb on + strand, within CA265_RS22660 at 5548.675 kb on + strand, within CA265_RS22660 at 5548.676 kb on - strand, within CA265_RS22660 at 5548.676 kb on - strand, within CA265_RS22660 at 5548.725 kb on + strand, within CA265_RS22660 at 5548.781 kb on + strand, within CA265_RS22660 at 5548.885 kb on - strand, within CA265_RS22660 at 5548.921 kb on + strand, within CA265_RS22660 at 5548.921 kb on + strand, within CA265_RS22660 at 5549.055 kb on - strand, within CA265_RS22660 at 5549.135 kb on - strand, within CA265_RS22660 at 5549.147 kb on + strand, within CA265_RS22660 at 5549.166 kb on - strand, within CA265_RS22660 at 5549.166 kb on - strand, within CA265_RS22660 at 5549.208 kb on - strand, within CA265_RS22660 at 5549.314 kb on + strand, within CA265_RS22660 at 5549.315 kb on - strand, within CA265_RS22660 at 5549.397 kb on - strand, within CA265_RS22660 at 5549.441 kb on + strand, within CA265_RS22660 at 5549.468 kb on - strand, within CA265_RS22660 at 5549.486 kb on + strand, within CA265_RS22660 at 5549.501 kb on - strand, within CA265_RS22660 at 5549.574 kb on - strand at 5549.582 kb on + strand at 5549.583 kb on - strand at 5549.764 kb on + strand at 5549.768 kb on + strand at 5549.768 kb on + strand at 5549.769 kb on - strand at 5549.888 kb on + strand at 5549.889 kb on - strand at 5549.889 kb on - strand at 5549.907 kb on - strand at 5550.084 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.01 mg/ml remove 5,547,380 + -0.5 5,547,380 + -0.3 5,547,381 - +0.1 5,547,382 + +0.3 5,547,420 + +0.4 5,547,421 - -0.5 5,547,477 + -1.0 5,547,520 + -0.6 5,547,693 + CA265_RS22655 0.37 -0.3 5,547,693 + CA265_RS22655 0.37 -0.4 5,547,693 + CA265_RS22655 0.37 +0.0 5,547,694 - CA265_RS22655 0.38 -0.5 5,547,762 + CA265_RS22655 0.52 +0.3 5,547,913 + CA265_RS22655 0.85 +0.4 5,547,914 + CA265_RS22655 0.85 -0.4 5,547,914 + CA265_RS22655 0.85 -1.2 5,547,915 - CA265_RS22655 0.85 +0.8 5,547,990 - +0.1 5,548,092 - +0.8 5,548,093 + +0.2 5,548,305 - +0.8 5,548,305 - +0.8 5,548,308 + +0.9 5,548,398 + -1.4 5,548,398 + -0.6 5,548,399 - +0.0 5,548,408 + -0.3 5,548,467 - CA265_RS22660 0.12 -0.4 5,548,493 + CA265_RS22660 0.14 -1.5 5,548,494 - CA265_RS22660 0.14 -2.5 5,548,610 - CA265_RS22660 0.22 -3.2 5,548,631 - CA265_RS22660 0.24 -0.4 5,548,675 + CA265_RS22660 0.27 +0.8 5,548,675 + CA265_RS22660 0.27 -0.2 5,548,676 - CA265_RS22660 0.27 +0.0 5,548,676 - CA265_RS22660 0.27 -1.8 5,548,725 + CA265_RS22660 0.30 -1.0 5,548,781 + CA265_RS22660 0.34 -3.6 5,548,885 - CA265_RS22660 0.42 +0.6 5,548,921 + CA265_RS22660 0.45 -1.6 5,548,921 + CA265_RS22660 0.45 -0.4 5,549,055 - CA265_RS22660 0.54 -1.1 5,549,135 - CA265_RS22660 0.60 -1.2 5,549,147 + CA265_RS22660 0.61 -0.8 5,549,166 - CA265_RS22660 0.62 -0.1 5,549,166 - CA265_RS22660 0.62 -2.1 5,549,208 - CA265_RS22660 0.65 -0.8 5,549,314 + CA265_RS22660 0.73 +0.5 5,549,315 - CA265_RS22660 0.73 -2.1 5,549,397 - CA265_RS22660 0.79 -0.4 5,549,441 + CA265_RS22660 0.82 -0.8 5,549,468 - CA265_RS22660 0.84 -1.1 5,549,486 + CA265_RS22660 0.86 -2.5 5,549,501 - CA265_RS22660 0.87 -1.4 5,549,574 - -0.6 5,549,582 + -0.1 5,549,583 - +0.2 5,549,764 + +0.5 5,549,768 + -0.7 5,549,768 + -0.1 5,549,769 - -0.0 5,549,888 + -2.5 5,549,889 - +2.0 5,549,889 - +0.4 5,549,907 - -0.3 5,550,084 + +0.9
Or see this region's nucleotide sequence