Experiment: R2A with Cisplatin 0.01 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04925 and CA265_RS04930 are separated by 558 nucleotides CA265_RS04930 and CA265_RS04935 are separated by 69 nucleotides CA265_RS04935 and CA265_RS04940 are separated by 94 nucleotides CA265_RS04940 and CA265_RS04945 are separated by 61 nucleotides
CA265_RS04925: CA265_RS04925 - hypothetical protein, at 1,174,963 to 1,175,778
_RS04925
CA265_RS04930: CA265_RS04930 - tRNA-Leu, at 1,176,337 to 1,176,420
_RS04930
CA265_RS04935: CA265_RS04935 - tRNA-Gly, at 1,176,490 to 1,176,565
_RS04935
CA265_RS04940: CA265_RS04940 - RecX family transcriptional regulator, at 1,176,660 to 1,177,148
_RS04940
CA265_RS04945: CA265_RS04945 - bifunctional UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine ligase/alanine racemase, at 1,177,210 to 1,179,669
_RS04945
Position (kb)
1176
1177
1178 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.661 kb on - strand, within CA265_RS04925 at 1175.661 kb on - strand, within CA265_RS04925 at 1175.844 kb on - strand at 1175.910 kb on - strand at 1175.910 kb on - strand at 1176.082 kb on + strand at 1176.085 kb on - strand at 1176.126 kb on + strand at 1176.141 kb on - strand at 1176.142 kb on + strand at 1176.621 kb on - strand at 1176.691 kb on - strand at 1176.727 kb on + strand, within CA265_RS04940 at 1176.814 kb on - strand, within CA265_RS04940 at 1176.839 kb on - strand, within CA265_RS04940 at 1176.924 kb on - strand, within CA265_RS04940 at 1176.941 kb on - strand, within CA265_RS04940 at 1177.040 kb on + strand, within CA265_RS04940 at 1177.041 kb on - strand, within CA265_RS04940 at 1177.042 kb on + strand, within CA265_RS04940 at 1177.048 kb on - strand, within CA265_RS04940 at 1177.116 kb on + strand at 1177.116 kb on + strand at 1177.119 kb on + strand at 1177.119 kb on + strand at 1177.122 kb on + strand at 1177.152 kb on + strand at 1177.204 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cisplatin 0.01 mg/ml remove 1,175,660 + CA265_RS04925 0.85 -0.9 1,175,660 + CA265_RS04925 0.85 +0.6 1,175,660 + CA265_RS04925 0.85 -0.2 1,175,660 + CA265_RS04925 0.85 +0.4 1,175,661 - CA265_RS04925 0.86 -0.2 1,175,661 - CA265_RS04925 0.86 -3.5 1,175,844 - -0.9 1,175,910 - -0.9 1,175,910 - -1.1 1,176,082 + -2.3 1,176,085 - -1.7 1,176,126 + -0.6 1,176,141 - -0.8 1,176,142 + -0.6 1,176,621 - -0.6 1,176,691 - +0.3 1,176,727 + CA265_RS04940 0.14 -0.1 1,176,814 - CA265_RS04940 0.31 +0.2 1,176,839 - CA265_RS04940 0.37 -1.7 1,176,924 - CA265_RS04940 0.54 -2.0 1,176,941 - CA265_RS04940 0.57 -2.2 1,177,040 + CA265_RS04940 0.78 -1.0 1,177,041 - CA265_RS04940 0.78 -2.5 1,177,042 + CA265_RS04940 0.78 -0.4 1,177,048 - CA265_RS04940 0.79 +0.2 1,177,116 + -0.3 1,177,116 + +0.7 1,177,119 + -0.8 1,177,119 + -0.7 1,177,122 + -0.2 1,177,152 + -0.5 1,177,204 + -0.4
Or see this region's nucleotide sequence