Experiment: KB with Berberine 250 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0820 and Psyr_0821 are separated by 355 nucleotides Psyr_0821 and Psyr_0822 overlap by 1 nucleotides Psyr_0822 and Psyr_0823 are separated by 12 nucleotides
Psyr_0820: Psyr_0820 - transcriptional regulator, LacI family, at 925,431 to 926,426
_0820
Psyr_0821: Psyr_0821 - PTS system D-fructose-specific IIA component (F1P-forming), Frc family / Phosphocarrier protein HPr / phosphoenolpyruvate--protein phosphotransferase, at 926,782 to 929,652
_0821
Psyr_0822: Psyr_0822 - 1-phosphofructokinase, at 929,652 to 930,593
_0822
Psyr_0823: Psyr_0823 - PTS system D-fructose-specific IIB component (F1P-forming), Frc family / PTS system D-fructose-specific IIC component (F1P-forming), Frc family, at 930,606 to 932,348
_0823
Position (kb)
926
927
928
929
930 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 926.162 kb on + strand, within Psyr_0820 at 926.200 kb on + strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.201 kb on - strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.246 kb on + strand, within Psyr_0820 at 926.247 kb on - strand, within Psyr_0820 at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.371 kb on + strand at 926.381 kb on + strand at 926.442 kb on + strand at 926.442 kb on + strand at 926.442 kb on + strand at 926.442 kb on + strand at 926.445 kb on + strand at 926.445 kb on + strand at 926.445 kb on + strand at 926.446 kb on - strand at 926.450 kb on - strand at 926.496 kb on + strand at 926.497 kb on - strand at 926.497 kb on - strand at 926.508 kb on + strand at 926.523 kb on + strand at 926.523 kb on + strand at 926.523 kb on + strand at 926.523 kb on + strand at 926.524 kb on - strand at 926.524 kb on - strand at 926.524 kb on - strand at 926.524 kb on - strand at 926.524 kb on - strand at 926.524 kb on - strand at 926.532 kb on + strand at 926.532 kb on + strand at 926.532 kb on + strand at 926.532 kb on + strand at 926.533 kb on - strand at 926.691 kb on - strand at 926.734 kb on + strand at 926.735 kb on - strand at 926.735 kb on - strand at 926.735 kb on - strand at 926.807 kb on + strand at 926.807 kb on + strand at 926.807 kb on + strand at 926.807 kb on + strand at 926.808 kb on - strand at 926.808 kb on - strand at 926.847 kb on + strand at 926.847 kb on + strand at 926.847 kb on + strand at 926.847 kb on + strand at 926.847 kb on + strand at 926.848 kb on - strand at 926.848 kb on - strand at 926.848 kb on - strand at 926.848 kb on - strand at 926.848 kb on - strand at 926.998 kb on - strand at 927.061 kb on + strand at 927.061 kb on + strand at 927.061 kb on + strand at 927.194 kb on + strand, within Psyr_0821 at 927.194 kb on + strand, within Psyr_0821 at 927.451 kb on + strand, within Psyr_0821 at 927.451 kb on + strand, within Psyr_0821 at 927.686 kb on + strand, within Psyr_0821 at 927.687 kb on - strand, within Psyr_0821 at 928.152 kb on + strand, within Psyr_0821 at 928.153 kb on - strand, within Psyr_0821 at 928.153 kb on - strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.429 kb on + strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.430 kb on - strand, within Psyr_0821 at 928.613 kb on + strand, within Psyr_0821 at 928.614 kb on - strand, within Psyr_0821 at 928.682 kb on + strand, within Psyr_0821 at 928.683 kb on - strand, within Psyr_0821 at 928.683 kb on - strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.320 kb on + strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.321 kb on - strand, within Psyr_0821 at 929.650 kb on + strand at 929.650 kb on + strand at 929.651 kb on - strand at 929.782 kb on + strand, within Psyr_0822 at 929.783 kb on - strand, within Psyr_0822 at 930.524 kb on + strand at 930.571 kb on + strand at 930.572 kb on - strand at 930.591 kb on + strand at 930.591 kb on + strand at 930.592 kb on - strand at 930.592 kb on - strand at 930.592 kb on - strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.619 kb on + strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.620 kb on - strand at 930.620 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Berberine 250 ug/ml remove 926,162 + Psyr_0820 0.73 +1.3 926,200 + Psyr_0820 0.77 -0.6 926,201 - Psyr_0820 0.77 +0.5 926,201 - Psyr_0820 0.77 +0.2 926,201 - Psyr_0820 0.77 -0.4 926,246 + Psyr_0820 0.82 +0.2 926,246 + Psyr_0820 0.82 -0.5 926,246 + Psyr_0820 0.82 -0.5 926,247 - Psyr_0820 0.82 -0.1 926,371 + -0.3 926,371 + -1.8 926,371 + -2.5 926,371 + +2.5 926,371 + -0.1 926,381 + -0.5 926,442 + +1.9 926,442 + -2.1 926,442 + -0.5 926,442 + -0.3 926,445 + -0.6 926,445 + +0.3 926,445 + -2.5 926,446 - +0.1 926,450 - +0.4 926,496 + +0.2 926,497 - -0.5 926,497 - +0.4 926,508 + +0.3 926,523 + +0.7 926,523 + +0.4 926,523 + -0.2 926,523 + -1.0 926,524 - +0.1 926,524 - -0.7 926,524 - -1.5 926,524 - +0.1 926,524 - -0.4 926,524 - +0.9 926,532 + -1.1 926,532 + -0.1 926,532 + -0.8 926,532 + +0.4 926,533 - +0.3 926,691 - +0.9 926,734 + +0.9 926,735 - +0.2 926,735 - -0.3 926,735 - +0.7 926,807 + +0.3 926,807 + +1.0 926,807 + -1.7 926,807 + -1.8 926,808 - +0.0 926,808 - -0.1 926,847 + +0.9 926,847 + -0.3 926,847 + +0.0 926,847 + -1.0 926,847 + +0.6 926,848 - -0.4 926,848 - +0.9 926,848 - -0.3 926,848 - -0.1 926,848 - -1.5 926,998 - -0.0 927,061 + +0.6 927,061 + -0.7 927,061 + -0.4 927,194 + Psyr_0821 0.14 +0.2 927,194 + Psyr_0821 0.14 -0.3 927,451 + Psyr_0821 0.23 -1.3 927,451 + Psyr_0821 0.23 -0.1 927,686 + Psyr_0821 0.31 -0.5 927,687 - Psyr_0821 0.32 -0.1 928,152 + Psyr_0821 0.48 -1.4 928,153 - Psyr_0821 0.48 +0.1 928,153 - Psyr_0821 0.48 -0.9 928,429 + Psyr_0821 0.57 +1.6 928,429 + Psyr_0821 0.57 -1.1 928,429 + Psyr_0821 0.57 -0.5 928,429 + Psyr_0821 0.57 -0.0 928,429 + Psyr_0821 0.57 -0.8 928,429 + Psyr_0821 0.57 +0.1 928,429 + Psyr_0821 0.57 -1.8 928,430 - Psyr_0821 0.57 -1.1 928,430 - Psyr_0821 0.57 +0.5 928,430 - Psyr_0821 0.57 -0.7 928,613 + Psyr_0821 0.64 -0.2 928,614 - Psyr_0821 0.64 -1.2 928,682 + Psyr_0821 0.66 -0.1 928,683 - Psyr_0821 0.66 -0.1 928,683 - Psyr_0821 0.66 +3.2 929,320 + Psyr_0821 0.88 -0.7 929,320 + Psyr_0821 0.88 -0.9 929,320 + Psyr_0821 0.88 +1.3 929,320 + Psyr_0821 0.88 -1.2 929,321 - Psyr_0821 0.88 -0.2 929,321 - Psyr_0821 0.88 -0.4 929,321 - Psyr_0821 0.88 -0.2 929,321 - Psyr_0821 0.88 +0.8 929,321 - Psyr_0821 0.88 +1.4 929,321 - Psyr_0821 0.88 -0.7 929,321 - Psyr_0821 0.88 -0.4 929,321 - Psyr_0821 0.88 +0.1 929,650 + +0.2 929,650 + -0.2 929,651 - -0.0 929,782 + Psyr_0822 0.14 +0.6 929,783 - Psyr_0822 0.14 -0.8 930,524 + +0.9 930,571 + -0.2 930,572 - +0.0 930,591 + -0.5 930,591 + +0.1 930,592 - -1.5 930,592 - +0.3 930,592 - +0.5 930,619 + -0.1 930,619 + +1.1 930,619 + -0.1 930,619 + +0.0 930,619 + -1.2 930,619 + -0.7 930,619 + -0.9 930,620 - +0.1 930,620 - -1.4 930,620 - +0.4 930,620 - -0.6
Or see this region's nucleotide sequence