Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4562 and Psyr_4563 are separated by 7 nucleotides Psyr_4563 and Psyr_4564 overlap by 11 nucleotides Psyr_4564 and Psyr_RNA70 are separated by 101 nucleotides Psyr_RNA70 and Psyr_RNA71 are separated by 149 nucleotides
Psyr_4562: Psyr_4562 - conserved domain protein, at 5,420,743 to 5,421,171
_4562
Psyr_4563: Psyr_4563 - Bvg accessory factor, at 5,421,179 to 5,421,928
_4563
Psyr_4564: Psyr_4564 - Biotin--acetyl-CoA-carboxylase ligase, at 5,421,918 to 5,422,925
_4564
Psyr_RNA70: Psyr_RNA70 - 5S ribosomal RNA, at 5,423,027 to 5,423,141
_RNA70
Psyr_RNA71: Psyr_RNA71 - 23S ribosomal RNA, at 5,423,291 to 5,426,193
_RNA71
Position (kb) 5421
5422
5423 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5420.981 kb on + strand, within Psyr_4562 at 5420.981 kb on + strand, within Psyr_4562 at 5420.982 kb on - strand, within Psyr_4562 at 5421.057 kb on + strand, within Psyr_4562 at 5421.058 kb on - strand, within Psyr_4562 at 5421.060 kb on + strand, within Psyr_4562 at 5421.060 kb on + strand, within Psyr_4562 at 5421.061 kb on - strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.126 kb on - strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.129 kb on - strand at 5421.138 kb on + strand at 5421.138 kb on + strand at 5421.160 kb on + strand at 5421.160 kb on + strand at 5421.161 kb on - strand at 5421.172 kb on - strand at 5422.693 kb on - strand, within Psyr_4564 at 5422.693 kb on - strand, within Psyr_4564 at 5422.693 kb on - strand, within Psyr_4564 at 5422.693 kb on - strand, within Psyr_4564 at 5422.693 kb on - strand, within Psyr_4564 at 5422.693 kb on - strand, within Psyr_4564 at 5422.719 kb on - strand, within Psyr_4564 at 5422.719 kb on - strand, within Psyr_4564 at 5422.719 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.750 kb on - strand, within Psyr_4564 at 5422.777 kb on - strand, within Psyr_4564 at 5422.777 kb on - strand, within Psyr_4564 at 5422.804 kb on - strand, within Psyr_4564 at 5422.822 kb on - strand, within Psyr_4564 at 5422.825 kb on - strand at 5422.825 kb on - strand at 5422.825 kb on - strand at 5422.825 kb on - strand at 5422.933 kb on - strand at 5422.933 kb on - strand at 5422.933 kb on - strand at 5422.933 kb on - strand at 5422.972 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 5,420,981 + Psyr_4562 0.55 -1.3 5,420,981 + Psyr_4562 0.55 -1.0 5,420,982 - Psyr_4562 0.56 +1.0 5,421,057 + Psyr_4562 0.73 -2.7 5,421,058 - Psyr_4562 0.73 -3.0 5,421,060 + Psyr_4562 0.74 -0.6 5,421,060 + Psyr_4562 0.74 -0.2 5,421,061 - Psyr_4562 0.74 -0.3 5,421,125 + Psyr_4562 0.89 +0.1 5,421,125 + Psyr_4562 0.89 -1.3 5,421,125 + Psyr_4562 0.89 -2.2 5,421,125 + Psyr_4562 0.89 -1.5 5,421,125 + Psyr_4562 0.89 -1.0 5,421,126 - Psyr_4562 0.89 -0.0 5,421,128 + Psyr_4562 0.90 -1.1 5,421,128 + Psyr_4562 0.90 -0.6 5,421,128 + Psyr_4562 0.90 -0.6 5,421,129 - +0.6 5,421,138 + -1.6 5,421,138 + -0.2 5,421,160 + -0.1 5,421,160 + +0.0 5,421,161 - +0.1 5,421,172 - +1.0 5,422,693 - Psyr_4564 0.77 -1.6 5,422,693 - Psyr_4564 0.77 -0.8 5,422,693 - Psyr_4564 0.77 -1.2 5,422,693 - Psyr_4564 0.77 +0.6 5,422,693 - Psyr_4564 0.77 -1.3 5,422,693 - Psyr_4564 0.77 +0.6 5,422,719 - Psyr_4564 0.79 -0.4 5,422,719 - Psyr_4564 0.79 -1.3 5,422,719 - Psyr_4564 0.79 -1.6 5,422,750 - Psyr_4564 0.83 -1.3 5,422,750 - Psyr_4564 0.83 -0.9 5,422,750 - Psyr_4564 0.83 -0.3 5,422,750 - Psyr_4564 0.83 +0.0 5,422,750 - Psyr_4564 0.83 -0.8 5,422,750 - Psyr_4564 0.83 +1.0 5,422,750 - Psyr_4564 0.83 -1.0 5,422,750 - Psyr_4564 0.83 +1.6 5,422,750 - +0.0 5,422,750 - Psyr_4564 0.83 -1.6 5,422,750 - Psyr_4564 0.83 -0.6 5,422,750 - Psyr_4564 0.83 -1.7 5,422,750 - Psyr_4564 0.83 +0.0 5,422,750 - Psyr_4564 0.83 -0.6 5,422,750 - Psyr_4564 0.83 -0.4 5,422,777 - Psyr_4564 0.85 -0.2 5,422,777 - Psyr_4564 0.85 +0.4 5,422,804 - Psyr_4564 0.88 +0.2 5,422,822 - Psyr_4564 0.90 -0.6 5,422,825 - -1.2 5,422,825 - +0.0 5,422,825 - -0.2 5,422,825 - +0.0 5,422,933 - +1.5 5,422,933 - +0.6 5,422,933 - +0.7 5,422,933 - -1.4 5,422,972 - -1.3
Or see this region's nucleotide sequence