Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4561 and Psyr_RNA66 are separated by 46 nucleotides Psyr_RNA66 and Psyr_RNA67 are separated by 165 nucleotides Psyr_RNA67 and Psyr_RNA68 are separated by 26 nucleotides Psyr_RNA68 and Psyr_RNA69 are separated by 24 nucleotides Psyr_RNA69 and Psyr_4562 are separated by 142 nucleotides Psyr_4562 and Psyr_4563 are separated by 7 nucleotides Psyr_4563 and Psyr_4564 overlap by 11 nucleotides
Psyr_4561: Psyr_4561 - protein translocase subunit secE/sec61 gamma, at 5,419,660 to 5,420,028
_4561
Psyr_RNA66: Psyr_RNA66 - tRNA-Trp, at 5,420,075 to 5,420,150
_RNA66
Psyr_RNA67: Psyr_RNA67 - tRNA-Thr, at 5,420,316 to 5,420,391
_RNA67
Psyr_RNA68: Psyr_RNA68 - tRNA-Gly, at 5,420,418 to 5,420,491
_RNA68
Psyr_RNA69: Psyr_RNA69 - tRNA-Tyr, at 5,420,516 to 5,420,600
_RNA69
Psyr_4562: Psyr_4562 - conserved domain protein, at 5,420,743 to 5,421,171
_4562
Psyr_4563: Psyr_4563 - Bvg accessory factor, at 5,421,179 to 5,421,928
_4563
Psyr_4564: Psyr_4564 - Biotin--acetyl-CoA-carboxylase ligase, at 5,421,918 to 5,422,925
_4564
Position (kb) 5420
5421
5422 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.061 kb on - strand at 5420.067 kb on - strand at 5420.067 kb on - strand at 5420.207 kb on - strand at 5420.278 kb on - strand at 5420.293 kb on - strand at 5420.293 kb on - strand at 5420.293 kb on - strand at 5420.301 kb on - strand at 5420.670 kb on - strand at 5420.670 kb on - strand at 5420.686 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.736 kb on - strand at 5420.740 kb on - strand at 5420.740 kb on - strand at 5420.777 kb on - strand at 5420.777 kb on - strand at 5420.871 kb on - strand, within Psyr_4562 at 5420.981 kb on + strand, within Psyr_4562 at 5420.981 kb on + strand, within Psyr_4562 at 5420.982 kb on - strand, within Psyr_4562 at 5421.057 kb on + strand, within Psyr_4562 at 5421.058 kb on - strand, within Psyr_4562 at 5421.060 kb on + strand, within Psyr_4562 at 5421.060 kb on + strand, within Psyr_4562 at 5421.061 kb on - strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.125 kb on + strand, within Psyr_4562 at 5421.126 kb on - strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.128 kb on + strand, within Psyr_4562 at 5421.129 kb on - strand at 5421.138 kb on + strand at 5421.138 kb on + strand at 5421.160 kb on + strand at 5421.160 kb on + strand at 5421.161 kb on - strand at 5421.172 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 5,420,061 - -1.5 5,420,061 - -2.0 5,420,061 - -0.6 5,420,061 - +1.0 5,420,061 - -1.1 5,420,061 - +3.3 5,420,061 - +1.7 5,420,061 - +0.4 5,420,061 - -0.3 5,420,067 - +0.2 5,420,067 - +1.6 5,420,207 - +0.7 5,420,278 - +0.9 5,420,293 - +1.3 5,420,293 - +1.0 5,420,293 - +0.7 5,420,301 - -0.6 5,420,670 - +1.0 5,420,670 - +0.9 5,420,686 - -1.0 5,420,736 - +2.3 5,420,736 - -0.7 5,420,736 - -1.1 5,420,736 - +0.0 5,420,736 - +1.0 5,420,740 - -0.7 5,420,740 - +0.0 5,420,777 - +0.4 5,420,777 - +1.0 5,420,871 - Psyr_4562 0.30 +0.4 5,420,981 + Psyr_4562 0.55 -1.3 5,420,981 + Psyr_4562 0.55 -1.0 5,420,982 - Psyr_4562 0.56 +1.0 5,421,057 + Psyr_4562 0.73 -2.7 5,421,058 - Psyr_4562 0.73 -3.0 5,421,060 + Psyr_4562 0.74 -0.6 5,421,060 + Psyr_4562 0.74 -0.2 5,421,061 - Psyr_4562 0.74 -0.3 5,421,125 + Psyr_4562 0.89 +0.1 5,421,125 + Psyr_4562 0.89 -1.3 5,421,125 + Psyr_4562 0.89 -2.2 5,421,125 + Psyr_4562 0.89 -1.5 5,421,125 + Psyr_4562 0.89 -1.0 5,421,126 - Psyr_4562 0.89 -0.0 5,421,128 + Psyr_4562 0.90 -1.1 5,421,128 + Psyr_4562 0.90 -0.6 5,421,128 + Psyr_4562 0.90 -0.6 5,421,129 - +0.6 5,421,138 + -1.6 5,421,138 + -0.2 5,421,160 + -0.1 5,421,160 + +0.0 5,421,161 - +0.1 5,421,172 - +1.0
Or see this region's nucleotide sequence