Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4458 and Psyr_4459 overlap by 4 nucleotides Psyr_4459 and Psyr_4460 are separated by 129 nucleotides Psyr_4460 and Psyr_4461 are separated by 21 nucleotides Psyr_4461 and Psyr_4462 are separated by 47 nucleotides
Psyr_4458: Psyr_4458 - Antitermination protein NusB, at 5,321,845 to 5,322,342
_4458
Psyr_4459: Psyr_4459 - 6,7-dimethyl-8-ribityllumazine synthase, at 5,322,339 to 5,322,815
_4459
Psyr_4460: Psyr_4460 - 3,4-dihydroxy-2-butanone 4-phosphate synthase / GTP cyclohydrolase II, at 5,322,945 to 5,324,036
_4460
Psyr_4461: Psyr_4461 - Lumazine-binding protein, at 5,324,058 to 5,324,720
_4461
Psyr_4462: Psyr_4462 - diaminohydroxyphosphoribosylaminopyrimidine deaminase / 5-amino-6-(5-phosphoribosylamino)uracil reductase, at 5,324,768 to 5,325,868
_4462
Position (kb) 5322
5323
5324
5325 Strain fitness (log2 ratio) -1
0
1
2
3 at 5322.830 kb on - strand at 5322.830 kb on - strand at 5322.833 kb on - strand at 5322.870 kb on - strand at 5322.870 kb on - strand at 5322.873 kb on - strand at 5322.873 kb on - strand at 5322.873 kb on - strand at 5322.943 kb on - strand at 5322.947 kb on - strand at 5322.962 kb on - strand at 5322.962 kb on - strand at 5322.962 kb on - strand at 5322.962 kb on - strand at 5323.082 kb on - strand, within Psyr_4460 at 5323.082 kb on - strand, within Psyr_4460 at 5323.082 kb on - strand, within Psyr_4460 at 5323.082 kb on - strand, within Psyr_4460 at 5323.086 kb on - strand, within Psyr_4460 at 5323.360 kb on + strand, within Psyr_4460 at 5324.072 kb on - strand at 5324.072 kb on - strand at 5324.770 kb on - strand at 5324.770 kb on - strand at 5324.770 kb on - strand at 5324.771 kb on + strand at 5324.772 kb on - strand at 5324.772 kb on - strand at 5324.772 kb on - strand at 5324.772 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 5,322,830 - +0.9 5,322,830 - -0.7 5,322,833 - +0.2 5,322,870 - +0.3 5,322,870 - -0.8 5,322,873 - +3.0 5,322,873 - +0.7 5,322,873 - +0.3 5,322,943 - +1.4 5,322,947 - -0.2 5,322,962 - -1.0 5,322,962 - -0.6 5,322,962 - -1.6 5,322,962 - -0.2 5,323,082 - Psyr_4460 0.13 -0.3 5,323,082 - Psyr_4460 0.13 -1.3 5,323,082 - Psyr_4460 0.13 -0.0 5,323,082 - Psyr_4460 0.13 -0.7 5,323,086 - Psyr_4460 0.13 +0.7 5,323,360 + Psyr_4460 0.38 +0.2 5,324,072 - +2.0 5,324,072 - +0.2 5,324,770 - -0.8 5,324,770 - +0.7 5,324,770 - -1.6 5,324,771 + +0.4 5,324,772 - +0.3 5,324,772 - -0.9 5,324,772 - +0.4 5,324,772 - -0.6
Or see this region's nucleotide sequence