Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3365 and Psyr_3366 are separated by 21 nucleotides Psyr_3366 and Psyr_3367 are separated by 252 nucleotides Psyr_3367 and Psyr_3368 are separated by 8 nucleotides
Psyr_3365: Psyr_3365 - Transcriptional regulator Ada / DNA-O6-methylguanine--protein-cysteine S-methyltransferase, at 4,023,774 to 4,024,928
_3365
Psyr_3366: Psyr_3366 - DNA-3-methyladenine glycosylase II, at 4,024,950 to 4,025,615
_3366
Psyr_3367: Psyr_3367 - Iron permease FTR1, at 4,025,868 to 4,026,722
_3367
Psyr_3368: Psyr_3368 - Protein of unknown function DUF451, at 4,026,731 to 4,027,942
_3368
Position (kb)
4025
4026
4027 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4025.003 kb on - strand at 4025.003 kb on - strand at 4025.003 kb on - strand at 4025.086 kb on + strand, within Psyr_3366 at 4025.086 kb on + strand, within Psyr_3366 at 4025.086 kb on + strand, within Psyr_3366 at 4025.086 kb on + strand, within Psyr_3366 at 4025.086 kb on + strand, within Psyr_3366 at 4025.086 kb on + strand, within Psyr_3366 at 4025.086 kb on + strand, within Psyr_3366 at 4025.086 kb on + strand, within Psyr_3366 at 4025.087 kb on - strand, within Psyr_3366 at 4025.087 kb on - strand, within Psyr_3366 at 4025.087 kb on - strand, within Psyr_3366 at 4025.087 kb on - strand, within Psyr_3366 at 4025.087 kb on - strand, within Psyr_3366 at 4025.087 kb on - strand, within Psyr_3366 at 4025.156 kb on - strand, within Psyr_3366 at 4025.156 kb on - strand, within Psyr_3366 at 4025.156 kb on - strand, within Psyr_3366 at 4025.156 kb on - strand, within Psyr_3366 at 4025.550 kb on + strand at 4025.649 kb on + strand at 4025.650 kb on - strand at 4025.650 kb on - strand at 4025.650 kb on - strand at 4025.798 kb on - strand at 4025.820 kb on + strand at 4025.821 kb on - strand at 4025.867 kb on + strand at 4025.867 kb on + strand at 4025.867 kb on + strand at 4025.868 kb on - strand at 4025.868 kb on - strand at 4025.868 kb on - strand at 4025.868 kb on - strand at 4025.868 kb on - strand at 4025.868 kb on - strand at 4025.868 kb on - strand at 4025.918 kb on + strand at 4025.918 kb on + strand at 4025.918 kb on + strand at 4025.918 kb on + strand at 4025.918 kb on + strand at 4025.919 kb on - strand at 4025.940 kb on + strand at 4025.992 kb on + strand, within Psyr_3367 at 4025.993 kb on - strand, within Psyr_3367 at 4026.271 kb on + strand, within Psyr_3367 at 4026.372 kb on + strand, within Psyr_3367 at 4026.372 kb on + strand, within Psyr_3367 at 4026.373 kb on - strand, within Psyr_3367 at 4026.373 kb on - strand, within Psyr_3367 at 4026.619 kb on - strand, within Psyr_3367 at 4026.619 kb on - strand, within Psyr_3367 at 4026.627 kb on + strand, within Psyr_3367 at 4026.627 kb on + strand, within Psyr_3367 at 4026.628 kb on - strand, within Psyr_3367 at 4026.839 kb on + strand at 4026.839 kb on + strand at 4026.839 kb on + strand at 4026.839 kb on + strand at 4026.839 kb on + strand at 4026.839 kb on + strand at 4026.839 kb on + strand at 4026.840 kb on - strand at 4026.840 kb on - strand at 4026.843 kb on - strand at 4026.843 kb on - strand at 4027.094 kb on + strand, within Psyr_3368 at 4027.095 kb on - strand, within Psyr_3368 at 4027.095 kb on - strand, within Psyr_3368 at 4027.102 kb on + strand, within Psyr_3368 at 4027.220 kb on + strand, within Psyr_3368 at 4027.229 kb on + strand, within Psyr_3368 at 4027.230 kb on - strand, within Psyr_3368 at 4027.230 kb on - strand, within Psyr_3368 at 4027.413 kb on - strand, within Psyr_3368 at 4027.413 kb on - strand, within Psyr_3368 at 4027.415 kb on + strand, within Psyr_3368 at 4027.415 kb on + strand, within Psyr_3368 at 4027.416 kb on - strand, within Psyr_3368 at 4027.627 kb on - strand, within Psyr_3368 at 4027.652 kb on + strand, within Psyr_3368
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 4,025,003 - +0.4 4,025,003 - +1.8 4,025,003 - +0.8 4,025,086 + Psyr_3366 0.20 -1.2 4,025,086 + Psyr_3366 0.20 -0.4 4,025,086 + Psyr_3366 0.20 +0.4 4,025,086 + Psyr_3366 0.20 -1.2 4,025,086 + Psyr_3366 0.20 +1.2 4,025,086 + Psyr_3366 0.20 +0.2 4,025,086 + Psyr_3366 0.20 -0.2 4,025,086 + Psyr_3366 0.20 +0.8 4,025,087 - Psyr_3366 0.21 -0.2 4,025,087 - Psyr_3366 0.21 +0.1 4,025,087 - Psyr_3366 0.21 -0.2 4,025,087 - Psyr_3366 0.21 -0.7 4,025,087 - Psyr_3366 0.21 -0.2 4,025,087 - Psyr_3366 0.21 +1.1 4,025,156 - Psyr_3366 0.31 -0.2 4,025,156 - Psyr_3366 0.31 -0.2 4,025,156 - Psyr_3366 0.31 -1.4 4,025,156 - Psyr_3366 0.31 +1.8 4,025,550 + -0.2 4,025,649 + -1.0 4,025,650 - -0.5 4,025,650 - -2.5 4,025,650 - +0.9 4,025,798 - -0.3 4,025,820 + +0.2 4,025,821 - -0.2 4,025,867 + -0.1 4,025,867 + -1.5 4,025,867 + -0.3 4,025,868 - -0.2 4,025,868 - -0.2 4,025,868 - +0.1 4,025,868 - -0.8 4,025,868 - -1.1 4,025,868 - +1.5 4,025,868 - +0.8 4,025,918 + +0.1 4,025,918 + -0.8 4,025,918 + +0.0 4,025,918 + -0.1 4,025,918 + +0.3 4,025,919 - +0.2 4,025,940 + -2.0 4,025,992 + Psyr_3367 0.15 -0.5 4,025,993 - Psyr_3367 0.15 -0.8 4,026,271 + Psyr_3367 0.47 -0.2 4,026,372 + Psyr_3367 0.59 +0.9 4,026,372 + Psyr_3367 0.59 +0.5 4,026,373 - Psyr_3367 0.59 -0.6 4,026,373 - Psyr_3367 0.59 -0.8 4,026,619 - Psyr_3367 0.88 -2.0 4,026,619 - Psyr_3367 0.88 -0.1 4,026,627 + Psyr_3367 0.89 -2.4 4,026,627 + Psyr_3367 0.89 -0.2 4,026,628 - Psyr_3367 0.89 -0.1 4,026,839 + -1.4 4,026,839 + +0.3 4,026,839 + +0.0 4,026,839 + -1.0 4,026,839 + +0.6 4,026,839 + -0.6 4,026,839 + -0.2 4,026,840 - +0.8 4,026,840 - +0.6 4,026,843 - +0.2 4,026,843 - -1.8 4,027,094 + Psyr_3368 0.30 +0.0 4,027,095 - Psyr_3368 0.30 -1.0 4,027,095 - Psyr_3368 0.30 -1.0 4,027,102 + Psyr_3368 0.31 +0.2 4,027,220 + Psyr_3368 0.40 -0.3 4,027,229 + Psyr_3368 0.41 +0.2 4,027,230 - Psyr_3368 0.41 -0.2 4,027,230 - Psyr_3368 0.41 -1.2 4,027,413 - Psyr_3368 0.56 -0.5 4,027,413 - Psyr_3368 0.56 +0.2 4,027,415 + Psyr_3368 0.56 +0.8 4,027,415 + Psyr_3368 0.56 -1.4 4,027,416 - Psyr_3368 0.57 +1.6 4,027,627 - Psyr_3368 0.74 +0.2 4,027,652 + Psyr_3368 0.76 +1.8
Or see this region's nucleotide sequence