Experiment: KB with Acriflavine 7.75 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2413 and Psyr_2414 are separated by 70 nucleotides Psyr_2414 and Psyr_2415 are separated by 319 nucleotides Psyr_2415 and Psyr_2416 are separated by 95 nucleotides
Psyr_2413: Psyr_2413 - succinate semialdehyde dehydrogenase, at 2,796,408 to 2,797,838
_2413
Psyr_2414: Psyr_2414 - murein-DD-endopeptidase, Serine peptidase, MEROPS family S11, at 2,797,909 to 2,798,793
_2414
Psyr_2415: Psyr_2415 - hypothetical protein, at 2,799,113 to 2,799,673
_2415
Psyr_2416: Psyr_2416 - conserved hypothetical protein, at 2,799,769 to 2,799,951
_2416
Position (kb)
2797
2798
2799 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2797.367 kb on + strand, within Psyr_2413 at 2797.368 kb on - strand, within Psyr_2413 at 2797.368 kb on - strand, within Psyr_2413 at 2797.397 kb on + strand, within Psyr_2413 at 2797.397 kb on + strand, within Psyr_2413 at 2797.398 kb on - strand, within Psyr_2413 at 2797.588 kb on + strand, within Psyr_2413 at 2797.588 kb on + strand, within Psyr_2413 at 2797.638 kb on + strand, within Psyr_2413 at 2797.638 kb on + strand, within Psyr_2413 at 2797.638 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.683 kb on + strand, within Psyr_2413 at 2797.684 kb on - strand, within Psyr_2413 at 2797.684 kb on - strand, within Psyr_2413 at 2797.684 kb on - strand, within Psyr_2413 at 2797.684 kb on - strand, within Psyr_2413 at 2797.684 kb on - strand, within Psyr_2413 at 2797.804 kb on - strand at 2797.830 kb on + strand at 2797.855 kb on + strand at 2797.964 kb on + strand at 2797.964 kb on + strand at 2797.964 kb on + strand at 2797.964 kb on + strand at 2797.965 kb on - strand at 2798.048 kb on + strand, within Psyr_2414 at 2798.048 kb on + strand, within Psyr_2414 at 2798.048 kb on + strand, within Psyr_2414 at 2798.048 kb on + strand, within Psyr_2414 at 2798.213 kb on + strand, within Psyr_2414 at 2798.213 kb on + strand, within Psyr_2414 at 2798.330 kb on + strand, within Psyr_2414 at 2798.331 kb on - strand, within Psyr_2414 at 2798.358 kb on - strand, within Psyr_2414 at 2798.675 kb on + strand, within Psyr_2414 at 2798.785 kb on - strand at 2798.802 kb on + strand at 2798.802 kb on + strand at 2798.806 kb on + strand at 2798.806 kb on + strand at 2798.806 kb on + strand at 2798.806 kb on + strand at 2798.807 kb on - strand at 2798.807 kb on - strand at 2798.807 kb on - strand at 2798.813 kb on - strand at 2798.813 kb on - strand at 2798.873 kb on - strand at 2798.906 kb on + strand at 2798.981 kb on + strand at 2798.981 kb on + strand at 2798.982 kb on - strand at 2799.041 kb on + strand at 2799.041 kb on + strand at 2799.199 kb on + strand, within Psyr_2415 at 2799.324 kb on + strand, within Psyr_2415 at 2799.324 kb on + strand, within Psyr_2415 at 2799.325 kb on - strand, within Psyr_2415 at 2799.325 kb on - strand, within Psyr_2415 at 2799.325 kb on - strand, within Psyr_2415 at 2799.344 kb on + strand, within Psyr_2415 at 2799.363 kb on - strand, within Psyr_2415 at 2799.450 kb on - strand, within Psyr_2415 at 2799.450 kb on - strand, within Psyr_2415
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml remove 2,797,367 + Psyr_2413 0.67 +1.9 2,797,368 - Psyr_2413 0.67 -0.9 2,797,368 - Psyr_2413 0.67 +1.2 2,797,397 + Psyr_2413 0.69 +1.8 2,797,397 + Psyr_2413 0.69 +1.5 2,797,398 - Psyr_2413 0.69 -0.3 2,797,588 + Psyr_2413 0.82 -0.1 2,797,588 + Psyr_2413 0.82 -1.2 2,797,638 + Psyr_2413 0.86 +0.3 2,797,638 + Psyr_2413 0.86 -1.1 2,797,638 + Psyr_2413 0.86 +1.1 2,797,683 + Psyr_2413 0.89 -0.1 2,797,683 + Psyr_2413 0.89 +0.0 2,797,683 + Psyr_2413 0.89 -0.8 2,797,683 + Psyr_2413 0.89 -0.9 2,797,683 + Psyr_2413 0.89 +1.3 2,797,683 + Psyr_2413 0.89 +1.3 2,797,683 + Psyr_2413 0.89 -0.1 2,797,683 + Psyr_2413 0.89 +1.0 2,797,683 + Psyr_2413 0.89 +0.7 2,797,683 + Psyr_2413 0.89 +0.2 2,797,684 - Psyr_2413 0.89 -1.6 2,797,684 - Psyr_2413 0.89 +0.8 2,797,684 - Psyr_2413 0.89 +0.3 2,797,684 - Psyr_2413 0.89 -1.1 2,797,684 - Psyr_2413 0.89 -0.6 2,797,804 - -0.2 2,797,830 + +0.1 2,797,855 + +0.6 2,797,964 + -0.9 2,797,964 + +0.8 2,797,964 + +0.8 2,797,964 + -0.0 2,797,965 - -0.2 2,798,048 + Psyr_2414 0.16 -1.2 2,798,048 + Psyr_2414 0.16 -2.6 2,798,048 + Psyr_2414 0.16 +0.6 2,798,048 + Psyr_2414 0.16 +0.3 2,798,213 + Psyr_2414 0.34 -1.0 2,798,213 + Psyr_2414 0.34 -0.6 2,798,330 + Psyr_2414 0.48 -1.1 2,798,331 - Psyr_2414 0.48 -0.0 2,798,358 - Psyr_2414 0.51 -0.8 2,798,675 + Psyr_2414 0.87 -0.6 2,798,785 - +1.8 2,798,802 + +0.0 2,798,802 + -0.9 2,798,806 + -0.8 2,798,806 + +1.2 2,798,806 + +0.0 2,798,806 + +0.3 2,798,807 - +0.4 2,798,807 - +0.3 2,798,807 - -1.0 2,798,813 - -0.8 2,798,813 - +0.6 2,798,873 - +0.4 2,798,906 + -0.5 2,798,981 + +1.0 2,798,981 + -0.1 2,798,982 - +0.8 2,799,041 + +0.4 2,799,041 + -0.2 2,799,199 + Psyr_2415 0.15 -0.6 2,799,324 + Psyr_2415 0.38 +0.8 2,799,324 + Psyr_2415 0.38 +0.6 2,799,325 - Psyr_2415 0.38 +3.1 2,799,325 - Psyr_2415 0.38 +0.8 2,799,325 - Psyr_2415 0.38 +0.2 2,799,344 + Psyr_2415 0.41 -0.8 2,799,363 - Psyr_2415 0.45 -0.1 2,799,450 - Psyr_2415 0.60 -0.4 2,799,450 - Psyr_2415 0.60 +0.3
Or see this region's nucleotide sequence