Strain Fitness in Pseudomonas syringae pv. syringae B728a around Psyr_0228

Experiment: KB with Acriflavine 7.75 ug/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsyr_0227 and Psyr_0228 are separated by 242 nucleotidesPsyr_0228 and Psyr_0229 overlap by 4 nucleotides Psyr_0227: Psyr_0227 - gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase, at 240,295 to 241,794 _0227 Psyr_0228: Psyr_0228 - Major facilitator superfamily, at 242,037 to 243,185 _0228 Psyr_0229: Psyr_0229 - Phospholipase D/Transphosphatidylase, at 243,182 to 244,762 _0229 Position (kb) 242 243 244Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 241.063 kb on + strand, within Psyr_0227at 241.063 kb on + strand, within Psyr_0227at 241.064 kb on - strand, within Psyr_0227at 241.064 kb on - strand, within Psyr_0227at 241.064 kb on - strand, within Psyr_0227at 241.064 kb on - strand, within Psyr_0227at 241.353 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.366 kb on + strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.367 kb on - strand, within Psyr_0227at 241.368 kb on + strand, within Psyr_0227at 241.368 kb on + strand, within Psyr_0227at 241.383 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.444 kb on + strand, within Psyr_0227at 241.445 kb on - strand, within Psyr_0227at 241.445 kb on - strand, within Psyr_0227at 241.445 kb on - strand, within Psyr_0227at 241.445 kb on - strand, within Psyr_0227at 241.445 kb on - strand, within Psyr_0227at 241.445 kb on - strand, within Psyr_0227at 241.445 kb on - strand, within Psyr_0227at 241.599 kb on - strand, within Psyr_0227at 241.659 kb on - strandat 241.659 kb on - strandat 241.672 kb on + strandat 241.759 kb on + strandat 241.759 kb on + strandat 241.759 kb on + strandat 241.760 kb on - strandat 241.760 kb on - strandat 241.760 kb on - strandat 241.760 kb on - strandat 241.760 kb on - strandat 241.792 kb on + strandat 241.792 kb on + strandat 241.792 kb on + strandat 241.793 kb on - strandat 241.793 kb on - strandat 241.793 kb on - strandat 241.991 kb on + strandat 241.991 kb on + strandat 241.992 kb on - strandat 241.992 kb on - strandat 242.049 kb on - strandat 242.052 kb on + strandat 242.052 kb on + strandat 242.052 kb on + strandat 242.052 kb on + strandat 242.052 kb on + strandat 242.052 kb on + strandat 242.053 kb on - strandat 242.053 kb on - strandat 242.053 kb on - strandat 242.053 kb on - strandat 242.053 kb on - strandat 242.307 kb on + strand, within Psyr_0228at 242.310 kb on + strand, within Psyr_0228at 242.433 kb on + strand, within Psyr_0228at 242.445 kb on + strand, within Psyr_0228at 242.445 kb on + strand, within Psyr_0228at 242.446 kb on - strand, within Psyr_0228at 242.446 kb on - strand, within Psyr_0228at 242.446 kb on - strand, within Psyr_0228at 242.446 kb on - strand, within Psyr_0228at 242.703 kb on + strand, within Psyr_0228at 242.704 kb on - strand, within Psyr_0228at 242.711 kb on - strand, within Psyr_0228at 242.711 kb on - strand, within Psyr_0228at 243.184 kb on - strandat 243.257 kb on - strandat 243.257 kb on - strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.327 kb on + strandat 243.328 kb on - strandat 243.328 kb on - strandat 243.328 kb on - strandat 243.328 kb on - strandat 243.328 kb on - strandat 243.328 kb on - strandat 243.633 kb on + strand, within Psyr_0229at 243.732 kb on + strand, within Psyr_0229at 243.732 kb on + strand, within Psyr_0229at 243.732 kb on + strand, within Psyr_0229at 243.732 kb on + strand, within Psyr_0229at 243.733 kb on - strand, within Psyr_0229at 243.733 kb on - strand, within Psyr_0229at 243.733 kb on - strand, within Psyr_0229at 243.762 kb on + strand, within Psyr_0229at 243.762 kb on + strand, within Psyr_0229at 243.762 kb on + strand, within Psyr_0229at 243.763 kb on - strand, within Psyr_0229at 243.763 kb on - strand, within Psyr_0229at 243.763 kb on - strand, within Psyr_0229at 243.860 kb on + strand, within Psyr_0229at 243.861 kb on - strand, within Psyr_0229at 243.861 kb on - strand, within Psyr_0229at 243.894 kb on + strand, within Psyr_0229at 243.894 kb on + strand, within Psyr_0229at 243.894 kb on + strand, within Psyr_0229at 243.894 kb on + strand, within Psyr_0229at 243.895 kb on - strand, within Psyr_0229at 243.895 kb on - strand, within Psyr_0229at 243.895 kb on - strand, within Psyr_0229at 243.895 kb on - strand, within Psyr_0229at 243.895 kb on - strand, within Psyr_0229at 243.945 kb on + strand, within Psyr_0229at 243.945 kb on + strand, within Psyr_0229at 243.945 kb on + strand, within Psyr_0229at 243.945 kb on + strand, within Psyr_0229at 243.946 kb on - strand, within Psyr_0229at 243.963 kb on + strand, within Psyr_0229at 243.963 kb on + strand, within Psyr_0229at 243.963 kb on + strand, within Psyr_0229at 243.964 kb on - strand, within Psyr_0229at 243.964 kb on - strand, within Psyr_0229at 243.964 kb on - strand, within Psyr_0229at 243.964 kb on - strand, within Psyr_0229at 244.053 kb on + strand, within Psyr_0229at 244.053 kb on + strand, within Psyr_0229at 244.053 kb on + strand, within Psyr_0229at 244.053 kb on + strand, within Psyr_0229at 244.054 kb on - strand, within Psyr_0229at 244.054 kb on - strand, within Psyr_0229at 244.054 kb on - strand, within Psyr_0229at 244.054 kb on - strand, within Psyr_0229at 244.054 kb on - strand, within Psyr_0229at 244.054 kb on - strand, within Psyr_0229at 244.096 kb on - strand, within Psyr_0229

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Per-strain Table

Position Strand Gene LocusTag Fraction KB with Acriflavine 7.75 ug/ml
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241,063 + Psyr_0227 0.51 +0.6
241,063 + Psyr_0227 0.51 +1.8
241,064 - Psyr_0227 0.51 -0.7
241,064 - Psyr_0227 0.51 +0.0
241,064 - Psyr_0227 0.51 -0.7
241,064 - Psyr_0227 0.51 -2.1
241,353 + Psyr_0227 0.71 +2.0
241,366 + Psyr_0227 0.71 +2.9
241,366 + Psyr_0227 0.71 +1.3
241,366 + Psyr_0227 0.71 +1.3
241,366 + Psyr_0227 0.71 +2.5
241,366 + Psyr_0227 0.71 +3.5
241,366 + Psyr_0227 0.71 +0.3
241,366 + Psyr_0227 0.71 +0.6
241,366 + Psyr_0227 0.71 +1.3
241,366 + Psyr_0227 0.71 +1.9
241,366 + Psyr_0227 0.71 +1.7
241,366 + Psyr_0227 0.71 -0.7
241,367 - Psyr_0227 0.71 -0.3
241,367 - Psyr_0227 0.71 -1.7
241,367 - Psyr_0227 0.71 +0.8
241,367 - Psyr_0227 0.71 +1.0
241,367 - Psyr_0227 0.71 -1.6
241,367 - Psyr_0227 0.71 -0.7
241,367 - Psyr_0227 0.71 -0.7
241,367 - Psyr_0227 0.71 -1.4
241,368 + Psyr_0227 0.72 +1.4
241,368 + Psyr_0227 0.72 +1.6
241,383 + Psyr_0227 0.73 +2.1
241,444 + Psyr_0227 0.77 +2.4
241,444 + Psyr_0227 0.77 +1.9
241,444 + Psyr_0227 0.77 +3.0
241,444 + Psyr_0227 0.77 +2.4
241,444 + Psyr_0227 0.77 +3.6
241,444 + Psyr_0227 0.77 +4.6
241,444 + Psyr_0227 0.77 +2.2
241,444 + Psyr_0227 0.77 +3.6
241,444 + Psyr_0227 0.77 +2.3
241,445 - Psyr_0227 0.77 -1.4
241,445 - Psyr_0227 0.77 -1.6
241,445 - Psyr_0227 0.77 -1.2
241,445 - Psyr_0227 0.77 -2.1
241,445 - Psyr_0227 0.77 -1.2
241,445 - Psyr_0227 0.77 -2.6
241,445 - Psyr_0227 0.77 -2.5
241,599 - Psyr_0227 0.87 -3.5
241,659 - -0.6
241,659 - -0.6
241,672 + +1.4
241,759 + +2.9
241,759 + +0.6
241,759 + +2.6
241,760 - -1.9
241,760 - -2.5
241,760 - -1.4
241,760 - -1.5
241,760 - +0.2
241,792 + +2.9
241,792 + +1.8
241,792 + +2.6
241,793 - -0.5
241,793 - -2.9
241,793 - -1.8
241,991 + -1.2
241,991 + -0.4
241,992 - -3.4
241,992 - +0.4
242,049 - -0.4
242,052 + -1.0
242,052 + -0.2
242,052 + -1.7
242,052 + -0.8
242,052 + +0.1
242,052 + -2.4
242,053 - -2.2
242,053 - -1.1
242,053 - -0.2
242,053 - -1.6
242,053 - -1.0
242,307 + Psyr_0228 0.23 -1.4
242,310 + Psyr_0228 0.24 -2.3
242,433 + Psyr_0228 0.34 -2.8
242,445 + Psyr_0228 0.36 -2.2
242,445 + Psyr_0228 0.36 -0.1
242,446 - Psyr_0228 0.36 -0.5
242,446 - Psyr_0228 0.36 -2.3
242,446 - Psyr_0228 0.36 -3.2
242,446 - Psyr_0228 0.36 -2.2
242,703 + Psyr_0228 0.58 -1.0
242,704 - Psyr_0228 0.58 -0.4
242,711 - Psyr_0228 0.59 +0.7
242,711 - Psyr_0228 0.59 -1.0
243,184 - -0.4
243,257 - +0.3
243,257 - +0.0
243,327 + -2.3
243,327 + +1.3
243,327 + +1.5
243,327 + -1.5
243,327 + +1.2
243,327 + -0.4
243,327 + -1.4
243,327 + +0.7
243,327 + -0.1
243,327 + -0.1
243,328 - +0.5
243,328 - +0.0
243,328 - -1.7
243,328 - -1.7
243,328 - -1.8
243,328 - -0.6
243,633 + Psyr_0229 0.29 -2.0
243,732 + Psyr_0229 0.35 -1.1
243,732 + Psyr_0229 0.35 -1.3
243,732 + Psyr_0229 0.35 -0.2
243,732 + Psyr_0229 0.35 -0.4
243,733 - Psyr_0229 0.35 -0.9
243,733 - Psyr_0229 0.35 -0.1
243,733 - Psyr_0229 0.35 -0.7
243,762 + Psyr_0229 0.37 -0.7
243,762 + Psyr_0229 0.37 -0.7
243,762 + Psyr_0229 0.37 +0.7
243,763 - Psyr_0229 0.37 -1.2
243,763 - Psyr_0229 0.37 +0.8
243,763 - Psyr_0229 0.37 +0.1
243,860 + Psyr_0229 0.43 +2.1
243,861 - Psyr_0229 0.43 -0.2
243,861 - Psyr_0229 0.43 -2.0
243,894 + Psyr_0229 0.45 +0.2
243,894 + Psyr_0229 0.45 -1.2
243,894 + Psyr_0229 0.45 -0.7
243,894 + Psyr_0229 0.45 -0.4
243,895 - Psyr_0229 0.45 -0.5
243,895 - Psyr_0229 0.45 +0.6
243,895 - Psyr_0229 0.45 +0.5
243,895 - Psyr_0229 0.45 -0.1
243,895 - Psyr_0229 0.45 -1.4
243,945 + Psyr_0229 0.48 -0.5
243,945 + Psyr_0229 0.48 -0.3
243,945 + Psyr_0229 0.48 +0.4
243,945 + Psyr_0229 0.48 -0.5
243,946 - Psyr_0229 0.48 +0.4
243,963 + Psyr_0229 0.49 +1.1
243,963 + Psyr_0229 0.49 -2.6
243,963 + Psyr_0229 0.49 -0.7
243,964 - Psyr_0229 0.49 -0.6
243,964 - Psyr_0229 0.49 +0.1
243,964 - Psyr_0229 0.49 -0.7
243,964 - Psyr_0229 0.49 -0.0
244,053 + Psyr_0229 0.55 -1.9
244,053 + Psyr_0229 0.55 -0.2
244,053 + Psyr_0229 0.55 +1.8
244,053 + Psyr_0229 0.55 +0.6
244,054 - Psyr_0229 0.55 -0.6
244,054 - Psyr_0229 0.55 +0.5
244,054 - Psyr_0229 0.55 -1.0
244,054 - Psyr_0229 0.55 +1.3
244,054 - Psyr_0229 0.55 -0.4
244,054 - Psyr_0229 0.55 -0.5
244,096 - Psyr_0229 0.58 -1.0

Or see this region's nucleotide sequence