Experiment: D-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1175 and Pf6N2E2_1176 are separated by 73 nucleotides Pf6N2E2_1176 and Pf6N2E2_1177 are separated by 12 nucleotides
Pf6N2E2_1175 - VgrG protein, at 1,375,292 to 1,377,517
_1175
Pf6N2E2_1176 - Mll2356 protein, at 1,377,591 to 1,377,986
_1176
Pf6N2E2_1177 - putative exported protein, at 1,377,999 to 1,379,141
_1177
Position (kb)
1377
1378 Strain fitness (log2 ratio)
-1
0
1
2 at 1377.197 kb on - strand, within Pf6N2E2_1175 at 1377.395 kb on - strand at 1377.395 kb on - strand at 1377.515 kb on + strand at 1377.515 kb on + strand at 1377.534 kb on - strand at 1377.546 kb on + strand at 1377.683 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.907 kb on + strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.908 kb on - strand, within Pf6N2E2_1176 at 1377.973 kb on + strand at 1378.037 kb on + strand at 1378.037 kb on + strand at 1378.038 kb on - strand at 1378.096 kb on - strand at 1378.169 kb on - strand, within Pf6N2E2_1177 at 1378.204 kb on - strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177 at 1378.356 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.602 kb on + strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.603 kb on - strand, within Pf6N2E2_1177 at 1378.649 kb on + strand, within Pf6N2E2_1177 at 1378.650 kb on - strand, within Pf6N2E2_1177 at 1378.668 kb on + strand, within Pf6N2E2_1177 at 1378.668 kb on + strand, within Pf6N2E2_1177 at 1378.669 kb on - strand, within Pf6N2E2_1177
Per-strain Table
Position Strand Gene LocusTag Fraction D-Lactate (C) remove 1,377,197 - Pf6N2E2_1175 0.86 -0.3 1,377,395 - +1.0 1,377,395 - -0.5 1,377,515 + -1.4 1,377,515 + -0.2 1,377,534 - +1.0 1,377,546 + -0.4 1,377,683 + Pf6N2E2_1176 0.23 -0.6 1,377,907 + Pf6N2E2_1176 0.80 -1.0 1,377,907 + Pf6N2E2_1176 0.80 +0.4 1,377,908 - Pf6N2E2_1176 0.80 -1.1 1,377,908 - Pf6N2E2_1176 0.80 +0.8 1,377,908 - Pf6N2E2_1176 0.80 -0.8 1,377,908 - Pf6N2E2_1176 0.80 -0.9 1,377,973 + -0.3 1,378,037 + -0.5 1,378,037 + -1.5 1,378,038 - +1.0 1,378,096 - -0.3 1,378,169 - Pf6N2E2_1177 0.15 -0.5 1,378,204 - Pf6N2E2_1177 0.18 -0.0 1,378,356 + Pf6N2E2_1177 0.31 +2.0 1,378,356 + Pf6N2E2_1177 0.31 -0.8 1,378,602 + Pf6N2E2_1177 0.53 -1.0 1,378,602 + Pf6N2E2_1177 0.53 +1.9 1,378,602 + Pf6N2E2_1177 0.53 +0.4 1,378,602 + Pf6N2E2_1177 0.53 +1.8 1,378,603 - Pf6N2E2_1177 0.53 +0.6 1,378,603 - Pf6N2E2_1177 0.53 -0.1 1,378,603 - Pf6N2E2_1177 0.53 +0.7 1,378,649 + Pf6N2E2_1177 0.57 +0.3 1,378,650 - Pf6N2E2_1177 0.57 -0.6 1,378,668 + Pf6N2E2_1177 0.59 -1.2 1,378,668 + Pf6N2E2_1177 0.59 -0.1 1,378,669 - Pf6N2E2_1177 0.59 +0.4
Or see this region's nucleotide sequence