Experiment: Cephalothin 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_3395 and Shew_3396 are separated by 169 nucleotides Shew_3396 and Shew_3397 are separated by 155 nucleotides Shew_3397 and Shew_3398 are separated by 366 nucleotides Shew_3398 and Shew_3399 are separated by 297 nucleotides
Shew_3395: Shew_3395 - radical SAM domain-containing protein (RefSeq), at 4,043,855 to 4,044,733
_3395
Shew_3396: Shew_3396 - hypothetical protein (RefSeq), at 4,044,903 to 4,045,205
_3396
Shew_3397: Shew_3397 - GCN5-related N-acetyltransferase (RefSeq), at 4,045,361 to 4,045,831
_3397
Shew_3398: Shew_3398 - cytochrome c, class I (RefSeq), at 4,046,198 to 4,046,494
_3398
Shew_3399: Shew_3399 - FMN-binding domain-containing protein (RefSeq), at 4,046,792 to 4,048,933
_3399
Position (kb)
4045
4046 Strain fitness (log2 ratio)
-1
0
1 at 4044.415 kb on + strand, within Shew_3395 at 4044.424 kb on + strand, within Shew_3395 at 4045.269 kb on + strand at 4045.713 kb on + strand, within Shew_3397 at 4045.721 kb on - strand, within Shew_3397 at 4045.771 kb on + strand, within Shew_3397 at 4045.800 kb on - strand at 4045.808 kb on + strand at 4045.964 kb on - strand at 4046.011 kb on + strand at 4046.332 kb on + strand, within Shew_3398 at 4046.600 kb on + strand at 4046.764 kb on + strand at 4046.764 kb on + strand at 4046.772 kb on - strand at 4046.772 kb on - strand at 4046.799 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.1 mg/ml remove 4,044,415 + Shew_3395 0.64 +0.2 4,044,424 + Shew_3395 0.65 +0.2 4,045,269 + -0.8 4,045,713 + Shew_3397 0.75 +0.6 4,045,721 - Shew_3397 0.76 -0.1 4,045,771 + Shew_3397 0.87 +1.0 4,045,800 - +0.4 4,045,808 + +0.3 4,045,964 - +0.2 4,046,011 + -0.2 4,046,332 + Shew_3398 0.45 -0.4 4,046,600 + -0.1 4,046,764 + -0.3 4,046,764 + +1.4 4,046,772 - -0.9 4,046,772 - +0.1 4,046,799 + +0.9
Or see this region's nucleotide sequence