Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_2087 and Dshi_2088 are separated by 373 nucleotides Dshi_2088 and Dshi_2089 are separated by 87 nucleotides Dshi_2089 and Dshi_2090 overlap by 4 nucleotides
Dshi_2087: Dshi_2087 - valyl-tRNA synthetase (RefSeq), at 2,209,300 to 2,212,413
_2087
Dshi_2088: Dshi_2088 - aminotransferase class I and II (RefSeq), at 2,212,787 to 2,213,905
_2088
Dshi_2089: Dshi_2089 - hypothetical protein (RefSeq), at 2,213,993 to 2,214,208
_2089
Dshi_2090: Dshi_2090 - Serine--glyoxylate transaminase (RefSeq), at 2,214,205 to 2,215,461
_2090
Position (kb)
2212
2213
2214 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2212.563 kb on + strand at 2212.571 kb on - strand at 2212.617 kb on - strand at 2212.965 kb on - strand, within Dshi_2088 at 2213.134 kb on - strand, within Dshi_2088 at 2213.240 kb on + strand, within Dshi_2088 at 2213.293 kb on + strand, within Dshi_2088 at 2213.295 kb on - strand, within Dshi_2088 at 2213.386 kb on - strand, within Dshi_2088 at 2213.476 kb on + strand, within Dshi_2088 at 2213.669 kb on + strand, within Dshi_2088 at 2213.723 kb on + strand, within Dshi_2088 at 2213.877 kb on - strand at 2214.050 kb on - strand, within Dshi_2089 at 2214.271 kb on + strand at 2214.325 kb on - strand at 2214.411 kb on - strand, within Dshi_2090 at 2214.536 kb on - strand, within Dshi_2090 at 2214.650 kb on + strand, within Dshi_2090 at 2214.704 kb on + strand, within Dshi_2090 at 2214.708 kb on + strand, within Dshi_2090 at 2214.742 kb on - strand, within Dshi_2090
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 2,212,563 + +2.0 2,212,571 - -2.3 2,212,617 - +0.3 2,212,965 - Dshi_2088 0.16 -3.0 2,213,134 - Dshi_2088 0.31 +2.6 2,213,240 + Dshi_2088 0.40 -1.9 2,213,293 + Dshi_2088 0.45 -0.3 2,213,295 - Dshi_2088 0.45 -2.0 2,213,386 - Dshi_2088 0.54 -2.0 2,213,476 + Dshi_2088 0.62 +0.3 2,213,669 + Dshi_2088 0.79 -0.9 2,213,723 + Dshi_2088 0.84 -3.2 2,213,877 - +0.6 2,214,050 - Dshi_2089 0.26 -2.3 2,214,271 + -1.3 2,214,325 - -1.7 2,214,411 - Dshi_2090 0.16 +2.4 2,214,536 - Dshi_2090 0.26 -2.7 2,214,650 + Dshi_2090 0.35 -0.4 2,214,704 + Dshi_2090 0.40 -2.1 2,214,708 + Dshi_2090 0.40 +1.5 2,214,742 - Dshi_2090 0.43 -1.3
Or see this region's nucleotide sequence