Experiment: m.b. Cobalt chloride 0.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Dshi_1301 and Dshi_1302 overlap by 4 nucleotides Dshi_1302 and Dshi_1303 overlap by 4 nucleotides Dshi_1303 and Dshi_1304 overlap by 4 nucleotides Dshi_1304 and Dshi_1305 are separated by 139 nucleotides
Dshi_1301: Dshi_1301 - Carbamoyl-phosphate synthase L chain ATP-binding (RefSeq), at 1,355,325 to 1,357,310
_1301
Dshi_1302: Dshi_1302 - Glutathione S-transferase domain (RefSeq), at 1,357,307 to 1,357,975
_1302
Dshi_1303: Dshi_1303 - Hydroxymethylglutaryl-CoA lyase (RefSeq), at 1,357,972 to 1,358,835
_1303
Dshi_1304: Dshi_1304 - Enoyl-CoA hydratase/isomerase (RefSeq), at 1,358,832 to 1,359,620
_1304
Dshi_1305: Dshi_1305 - 17 kDa surface antigen (RefSeq), at 1,359,760 to 1,359,978
_1305
Position (kb)
1357
1358
1359 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1357.076 kb on + strand, within Dshi_1301 at 1357.220 kb on + strand at 1357.244 kb on - strand at 1357.491 kb on - strand, within Dshi_1302 at 1357.528 kb on + strand, within Dshi_1302 at 1357.528 kb on + strand, within Dshi_1302 at 1357.679 kb on + strand, within Dshi_1302 at 1357.689 kb on + strand, within Dshi_1302 at 1357.766 kb on + strand, within Dshi_1302 at 1357.803 kb on - strand, within Dshi_1302 at 1358.158 kb on - strand, within Dshi_1303 at 1358.299 kb on + strand, within Dshi_1303 at 1358.501 kb on + strand, within Dshi_1303 at 1358.567 kb on + strand at 1358.816 kb on + strand at 1359.088 kb on - strand, within Dshi_1304 at 1359.308 kb on - strand, within Dshi_1304 at 1359.308 kb on - strand, within Dshi_1304 at 1359.537 kb on - strand at 1359.638 kb on + strand at 1359.658 kb on + strand at 1359.725 kb on - strand at 1359.798 kb on + strand, within Dshi_1305 at 1359.801 kb on - strand, within Dshi_1305 at 1359.811 kb on - strand, within Dshi_1305
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Cobalt chloride 0.5 mM remove 1,357,076 + Dshi_1301 0.88 -2.9 1,357,220 + +0.3 1,357,244 - -1.0 1,357,491 - Dshi_1302 0.28 -0.7 1,357,528 + Dshi_1302 0.33 +2.5 1,357,528 + Dshi_1302 0.33 -1.7 1,357,679 + Dshi_1302 0.56 +0.7 1,357,689 + Dshi_1302 0.57 +0.3 1,357,766 + Dshi_1302 0.69 +1.9 1,357,803 - Dshi_1302 0.74 -0.7 1,358,158 - Dshi_1303 0.22 -0.4 1,358,299 + Dshi_1303 0.38 -1.3 1,358,501 + Dshi_1303 0.61 -3.7 1,358,567 + +0.3 1,358,816 + -2.0 1,359,088 - Dshi_1304 0.32 -2.0 1,359,308 - Dshi_1304 0.60 +0.3 1,359,308 - Dshi_1304 0.60 +0.3 1,359,537 - +0.3 1,359,638 + +0.3 1,359,658 + -1.1 1,359,725 - -2.7 1,359,798 + Dshi_1305 0.17 +0.8 1,359,801 - Dshi_1305 0.19 +0.3 1,359,811 - Dshi_1305 0.23 -2.5
Or see this region's nucleotide sequence