Experiment: Fumarate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1819 and prpB are separated by 12 nucleotides prpB and Shew_1821 are separated by 101 nucleotides Shew_1821 and Shew_1822 are separated by 86 nucleotides
Shew_1819: Shew_1819 - GntR family transcriptional regulator (RefSeq), at 2,092,309 to 2,092,977
_1819
Shew_1820: prpB - 2-methylisocitrate lyase (RefSeq), at 2,092,990 to 2,093,868
prpB
Shew_1821: Shew_1821 - 2-methylcitrate synthase/citrate synthase II (RefSeq), at 2,093,970 to 2,095,094
_1821
Shew_1822: Shew_1822 - aconitate hydratase (RefSeq), at 2,095,181 to 2,097,769
_1822
Position (kb)
2093
2094
2095
2096 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2093.552 kb on - strand, within prpB at 2093.582 kb on + strand, within prpB at 2093.677 kb on - strand, within prpB at 2093.681 kb on + strand, within prpB at 2093.702 kb on - strand, within prpB at 2093.933 kb on + strand at 2093.933 kb on + strand at 2093.941 kb on - strand at 2094.037 kb on - strand at 2094.445 kb on + strand, within Shew_1821 at 2094.804 kb on - strand, within Shew_1821 at 2095.654 kb on - strand, within Shew_1822
Per-strain Table
Position Strand Gene LocusTag Fraction Fumarate (C) remove 2,093,552 - prpB Shew_1820 0.64 -1.2 2,093,582 + prpB Shew_1820 0.67 +4.8 2,093,677 - prpB Shew_1820 0.78 -1.8 2,093,681 + prpB Shew_1820 0.79 +5.6 2,093,702 - prpB Shew_1820 0.81 -1.4 2,093,933 + -0.5 2,093,933 + +0.5 2,093,941 - -1.0 2,094,037 - -2.0 2,094,445 + Shew_1821 0.42 -2.0 2,094,804 - Shew_1821 0.74 -3.0 2,095,654 - Shew_1822 0.18 +2.4
Or see this region's nucleotide sequence