Experiment: Paraquat dichloride 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sapD and sapC are separated by 3 nucleotides sapC and sapB overlap by 14 nucleotides sapB and sapA overlap by 1 nucleotides
SO1802: sapD - peptide ABC transporter, ATP-binding protein (NCBI ptt file), at 1,891,945 to 1,892,952
sapD
SO1803: sapC - peptide ABC transporter, permease protein (NCBI ptt file), at 1,892,956 to 1,893,846
sapC
SO1804: sapB - peptide ABC transporter, permease protein (NCBI ptt file), at 1,893,833 to 1,894,864
sapB
SO1805: sapA - peptide ABC transporter, periplasmic peptide-binding protein (NCBI ptt file), at 1,894,864 to 1,896,441
sapA
Position (kb)
1893
1894
1895 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1893.053 kb on + strand, within sapC at 1893.067 kb on + strand, within sapC at 1893.527 kb on - strand, within sapC at 1893.535 kb on + strand, within sapC at 1893.751 kb on - strand, within sapC at 1894.134 kb on + strand, within sapB at 1894.137 kb on - strand, within sapB at 1894.212 kb on - strand, within sapB at 1894.710 kb on + strand, within sapB at 1894.747 kb on - strand, within sapB at 1894.753 kb on + strand, within sapB at 1894.761 kb on - strand at 1894.761 kb on - strand at 1894.778 kb on - strand at 1894.984 kb on - strand at 1894.991 kb on + strand at 1895.567 kb on - strand, within sapA
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.25 mg/ml remove 1,893,053 + sapC SO1803 0.11 -3.0 1,893,067 + sapC SO1803 0.12 -2.8 1,893,527 - sapC SO1803 0.64 -2.3 1,893,535 + sapC SO1803 0.65 -2.9 1,893,751 - sapC SO1803 0.89 -3.5 1,894,134 + sapB SO1804 0.29 -2.8 1,894,137 - sapB SO1804 0.29 -3.2 1,894,212 - sapB SO1804 0.37 -2.2 1,894,710 + sapB SO1804 0.85 -2.2 1,894,747 - sapB SO1804 0.89 -2.5 1,894,753 + sapB SO1804 0.89 -2.6 1,894,761 - -3.0 1,894,761 - -3.9 1,894,778 - -2.8 1,894,984 - -2.0 1,894,991 + -1.8 1,895,567 - sapA SO1805 0.45 -3.7
Or see this region's nucleotide sequence