Experiment: Paraquat dichloride 0.25 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ahpF and ahpC are separated by 234 nucleotides ahpC and pepA-1 are separated by 341 nucleotides
SO0956: ahpF - alkyl hydroperoxide reductase, F subunit (NCBI ptt file), at 991,215 to 992,798
ahpF
SO0958: ahpC - alkyl hydroperoxide reductase, C subunit (NCBI ptt file), at 993,033 to 993,602
ahpC
SO0959: pepA-1 - cytosol aminopeptidase (NCBI ptt file), at 993,944 to 995,479
pepA-1
Position (kb) 993
994 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 992.102 kb on - strand, within ahpF at 992.289 kb on + strand, within ahpF at 992.382 kb on + strand, within ahpF at 992.424 kb on + strand, within ahpF at 992.507 kb on + strand, within ahpF at 992.507 kb on - strand, within ahpF at 992.534 kb on - strand, within ahpF at 992.554 kb on + strand, within ahpF at 992.591 kb on + strand, within ahpF at 992.596 kb on + strand, within ahpF at 992.596 kb on + strand, within ahpF at 992.596 kb on + strand, within ahpF at 992.604 kb on - strand, within ahpF at 992.604 kb on - strand, within ahpF at 992.663 kb on + strand at 992.673 kb on + strand at 992.812 kb on - strand at 992.854 kb on + strand at 992.999 kb on + strand at 993.050 kb on + strand at 993.050 kb on + strand at 993.058 kb on - strand at 993.062 kb on + strand at 993.087 kb on + strand at 993.134 kb on - strand, within ahpC at 993.136 kb on + strand, within ahpC at 993.171 kb on + strand, within ahpC at 993.230 kb on - strand, within ahpC at 993.242 kb on + strand, within ahpC at 993.242 kb on + strand, within ahpC at 993.250 kb on - strand, within ahpC at 993.250 kb on - strand, within ahpC at 993.257 kb on - strand, within ahpC at 993.356 kb on - strand, within ahpC at 993.395 kb on + strand, within ahpC at 993.441 kb on + strand, within ahpC at 993.549 kb on - strand at 993.590 kb on - strand at 993.682 kb on - strand at 993.684 kb on - strand at 993.784 kb on + strand at 993.921 kb on - strand at 993.970 kb on + strand at 994.068 kb on + strand at 994.076 kb on - strand at 994.076 kb on - strand at 994.130 kb on + strand, within pepA-1 at 994.234 kb on + strand, within pepA-1 at 994.298 kb on - strand, within pepA-1 at 994.391 kb on + strand, within pepA-1 at 994.431 kb on - strand, within pepA-1 at 994.559 kb on + strand, within pepA-1 at 994.567 kb on - strand, within pepA-1 at 994.590 kb on + strand, within pepA-1
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.25 mg/ml remove 992,102 - ahpF SO0956 0.56 -0.6 992,289 + ahpF SO0956 0.68 -0.2 992,382 + ahpF SO0956 0.74 -0.6 992,424 + ahpF SO0956 0.76 -0.4 992,507 + ahpF SO0956 0.82 -1.3 992,507 - ahpF SO0956 0.82 -1.5 992,534 - ahpF SO0956 0.83 -0.9 992,554 + ahpF SO0956 0.85 -0.5 992,591 + ahpF SO0956 0.87 -1.3 992,596 + ahpF SO0956 0.87 +0.1 992,596 + ahpF SO0956 0.87 -0.4 992,596 + ahpF SO0956 0.87 -0.6 992,604 - ahpF SO0956 0.88 -1.4 992,604 - ahpF SO0956 0.88 -0.7 992,663 + -0.3 992,673 + -0.1 992,812 - -1.5 992,854 + -0.6 992,999 + -0.8 993,050 + -0.6 993,050 + -0.4 993,058 - -1.6 993,062 + -0.2 993,087 + -3.9 993,134 - ahpC SO0958 0.18 -0.9 993,136 + ahpC SO0958 0.18 -2.5 993,171 + ahpC SO0958 0.24 -0.5 993,230 - ahpC SO0958 0.35 -0.2 993,242 + ahpC SO0958 0.37 -2.1 993,242 + ahpC SO0958 0.37 -1.5 993,250 - ahpC SO0958 0.38 -2.3 993,250 - ahpC SO0958 0.38 -0.7 993,257 - ahpC SO0958 0.39 -0.2 993,356 - ahpC SO0958 0.57 -0.8 993,395 + ahpC SO0958 0.64 -2.3 993,441 + ahpC SO0958 0.72 -2.9 993,549 - -2.4 993,590 - -3.5 993,682 - -1.3 993,684 - -2.2 993,784 + +0.6 993,921 - -0.6 993,970 + +0.8 994,068 + +0.4 994,076 - +0.8 994,076 - -0.2 994,130 + pepA-1 SO0959 0.12 -0.0 994,234 + pepA-1 SO0959 0.19 -0.6 994,298 - pepA-1 SO0959 0.23 -0.5 994,391 + pepA-1 SO0959 0.29 +0.5 994,431 - pepA-1 SO0959 0.32 +0.0 994,559 + pepA-1 SO0959 0.40 -0.5 994,567 - pepA-1 SO0959 0.41 -0.8 994,590 + pepA-1 SO0959 0.42 +0.0
Or see this region's nucleotide sequence