Experiment: Papa dilution 10-1 cacl2 maltose
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsG and fhuE are separated by 59 nucleotides fhuE and ycfF are separated by 333 nucleotides ycfF and ycfL are separated by 2 nucleotides
b1101: ptsG - fused glucose-specific PTS enzymes: IIB component/IIC component (NCBI), at 1,157,092 to 1,158,525
ptsG
b1102: fhuE - ferric-rhodotorulic acid outer membrane transporter (NCBI), at 1,158,585 to 1,160,774
fhuE
b1103: ycfF - orf, hypothetical protein (VIMSS), at 1,161,108 to 1,161,467
ycfF
b1104: ycfL - hypothetical protein (NCBI), at 1,161,470 to 1,161,847
ycfL
Position (kb)
1158
1159
1160
1161 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1157.625 kb on + strand, within ptsG at 1157.629 kb on - strand, within ptsG at 1157.629 kb on - strand, within ptsG at 1157.644 kb on - strand, within ptsG at 1157.650 kb on + strand, within ptsG at 1157.651 kb on - strand, within ptsG at 1157.671 kb on - strand, within ptsG at 1157.727 kb on - strand, within ptsG at 1157.761 kb on + strand, within ptsG at 1157.901 kb on - strand, within ptsG at 1157.901 kb on - strand, within ptsG at 1157.944 kb on - strand, within ptsG at 1157.945 kb on + strand, within ptsG at 1157.946 kb on - strand, within ptsG at 1157.946 kb on - strand, within ptsG at 1157.965 kb on + strand, within ptsG at 1157.989 kb on + strand, within ptsG at 1157.989 kb on + strand, within ptsG at 1158.000 kb on + strand, within ptsG at 1158.000 kb on + strand, within ptsG at 1158.027 kb on - strand, within ptsG at 1158.027 kb on - strand, within ptsG at 1158.048 kb on - strand, within ptsG at 1158.158 kb on - strand, within ptsG at 1158.265 kb on - strand, within ptsG at 1158.309 kb on - strand, within ptsG at 1158.347 kb on + strand, within ptsG at 1158.353 kb on + strand, within ptsG at 1158.552 kb on - strand at 1158.635 kb on - strand at 1158.686 kb on - strand at 1158.929 kb on + strand, within fhuE at 1159.005 kb on + strand, within fhuE at 1159.053 kb on + strand, within fhuE at 1159.058 kb on + strand, within fhuE at 1159.069 kb on + strand, within fhuE at 1159.077 kb on - strand, within fhuE at 1159.077 kb on - strand, within fhuE at 1159.077 kb on - strand, within fhuE at 1159.084 kb on + strand, within fhuE at 1159.139 kb on - strand, within fhuE at 1159.139 kb on - strand, within fhuE at 1159.245 kb on + strand, within fhuE at 1159.245 kb on + strand, within fhuE at 1159.245 kb on + strand, within fhuE at 1159.388 kb on + strand, within fhuE at 1159.391 kb on - strand, within fhuE at 1159.405 kb on + strand, within fhuE at 1159.410 kb on - strand, within fhuE at 1159.424 kb on - strand, within fhuE at 1159.424 kb on - strand, within fhuE at 1159.442 kb on - strand, within fhuE at 1159.446 kb on + strand, within fhuE at 1159.496 kb on - strand, within fhuE at 1159.496 kb on - strand, within fhuE at 1159.526 kb on + strand, within fhuE at 1159.570 kb on + strand, within fhuE at 1159.656 kb on + strand, within fhuE at 1159.664 kb on - strand, within fhuE at 1159.664 kb on - strand, within fhuE at 1159.699 kb on + strand, within fhuE at 1159.715 kb on + strand, within fhuE at 1159.715 kb on + strand, within fhuE at 1159.734 kb on + strand, within fhuE at 1159.785 kb on + strand, within fhuE at 1159.874 kb on - strand, within fhuE at 1159.883 kb on + strand, within fhuE at 1159.883 kb on + strand, within fhuE at 1159.883 kb on + strand, within fhuE at 1159.902 kb on + strand, within fhuE at 1159.930 kb on - strand, within fhuE at 1159.993 kb on + strand, within fhuE at 1160.056 kb on + strand, within fhuE at 1160.075 kb on - strand, within fhuE at 1160.075 kb on - strand, within fhuE at 1160.075 kb on - strand, within fhuE at 1160.304 kb on - strand, within fhuE at 1160.304 kb on - strand, within fhuE at 1160.420 kb on - strand, within fhuE at 1160.735 kb on - strand at 1160.799 kb on + strand at 1161.023 kb on + strand at 1161.023 kb on + strand at 1161.034 kb on + strand at 1161.048 kb on - strand at 1161.048 kb on - strand at 1161.180 kb on - strand, within ycfF at 1161.414 kb on - strand, within ycfF at 1161.508 kb on - strand, within ycfL at 1161.648 kb on - strand, within ycfL at 1161.659 kb on + strand, within ycfL
Per-strain Table
Position Strand Gene LocusTag Fraction Papa dilution 10-1 cacl2 maltose remove 1,157,625 + ptsG b1101 0.37 -0.3 1,157,629 - ptsG b1101 0.37 +0.0 1,157,629 - ptsG b1101 0.37 -1.3 1,157,644 - ptsG b1101 0.38 +0.4 1,157,650 + ptsG b1101 0.39 -0.4 1,157,651 - ptsG b1101 0.39 -0.6 1,157,671 - ptsG b1101 0.40 -0.6 1,157,727 - ptsG b1101 0.44 -0.3 1,157,761 + ptsG b1101 0.47 -0.0 1,157,901 - ptsG b1101 0.56 +0.6 1,157,901 - ptsG b1101 0.56 +0.4 1,157,944 - ptsG b1101 0.59 +0.1 1,157,945 + ptsG b1101 0.59 -0.1 1,157,946 - ptsG b1101 0.60 -0.1 1,157,946 - ptsG b1101 0.60 +0.4 1,157,965 + ptsG b1101 0.61 -0.6 1,157,989 + ptsG b1101 0.63 -0.7 1,157,989 + ptsG b1101 0.63 -0.6 1,158,000 + ptsG b1101 0.63 +0.8 1,158,000 + ptsG b1101 0.63 +1.5 1,158,027 - ptsG b1101 0.65 +0.4 1,158,027 - ptsG b1101 0.65 +0.0 1,158,048 - ptsG b1101 0.67 +0.3 1,158,158 - ptsG b1101 0.74 -0.2 1,158,265 - ptsG b1101 0.82 -2.3 1,158,309 - ptsG b1101 0.85 -1.7 1,158,347 + ptsG b1101 0.88 +0.2 1,158,353 + ptsG b1101 0.88 -1.7 1,158,552 - +0.0 1,158,635 - -0.6 1,158,686 - +0.7 1,158,929 + fhuE b1102 0.16 +0.7 1,159,005 + fhuE b1102 0.19 +0.7 1,159,053 + fhuE b1102 0.21 -0.2 1,159,058 + fhuE b1102 0.22 -0.3 1,159,069 + fhuE b1102 0.22 +0.1 1,159,077 - fhuE b1102 0.22 -0.3 1,159,077 - fhuE b1102 0.22 -2.2 1,159,077 - fhuE b1102 0.22 -1.2 1,159,084 + fhuE b1102 0.23 -2.0 1,159,139 - fhuE b1102 0.25 +0.3 1,159,139 - fhuE b1102 0.25 +0.6 1,159,245 + fhuE b1102 0.30 -0.9 1,159,245 + fhuE b1102 0.30 +2.6 1,159,245 + fhuE b1102 0.30 +2.3 1,159,388 + fhuE b1102 0.37 -0.1 1,159,391 - fhuE b1102 0.37 -0.7 1,159,405 + fhuE b1102 0.37 -1.0 1,159,410 - fhuE b1102 0.38 -1.0 1,159,424 - fhuE b1102 0.38 -0.1 1,159,424 - fhuE b1102 0.38 +2.2 1,159,442 - fhuE b1102 0.39 +0.3 1,159,446 + fhuE b1102 0.39 +1.1 1,159,496 - fhuE b1102 0.42 -0.5 1,159,496 - fhuE b1102 0.42 +0.6 1,159,526 + fhuE b1102 0.43 +0.1 1,159,570 + fhuE b1102 0.45 -0.3 1,159,656 + fhuE b1102 0.49 -0.0 1,159,664 - fhuE b1102 0.49 -0.2 1,159,664 - fhuE b1102 0.49 -0.7 1,159,699 + fhuE b1102 0.51 +0.4 1,159,715 + fhuE b1102 0.52 +0.4 1,159,715 + fhuE b1102 0.52 +0.8 1,159,734 + fhuE b1102 0.52 +1.1 1,159,785 + fhuE b1102 0.55 -0.0 1,159,874 - fhuE b1102 0.59 +0.2 1,159,883 + fhuE b1102 0.59 +1.6 1,159,883 + fhuE b1102 0.59 +0.6 1,159,883 + fhuE b1102 0.59 +2.0 1,159,902 + fhuE b1102 0.60 +0.8 1,159,930 - fhuE b1102 0.61 +0.3 1,159,993 + fhuE b1102 0.64 -0.1 1,160,056 + fhuE b1102 0.67 -0.2 1,160,075 - fhuE b1102 0.68 -0.5 1,160,075 - fhuE b1102 0.68 -0.1 1,160,075 - fhuE b1102 0.68 +1.0 1,160,304 - fhuE b1102 0.78 +2.0 1,160,304 - fhuE b1102 0.78 -1.2 1,160,420 - fhuE b1102 0.84 +0.6 1,160,735 - +0.3 1,160,799 + +1.6 1,161,023 + +0.5 1,161,023 + +0.7 1,161,034 + +0.7 1,161,048 - +1.5 1,161,048 - +0.1 1,161,180 - ycfF b1103 0.20 +2.0 1,161,414 - ycfF b1103 0.85 +1.7 1,161,508 - ycfL b1104 0.10 -0.3 1,161,648 - ycfL b1104 0.47 -1.1 1,161,659 + ycfL b1104 0.50 +1.4
Or see this region's nucleotide sequence