Experiment: Temperature shift 10_to_34; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_4912 and PP_4913 are separated by 13 nucleotides PP_4913 and PP_4914 overlap by 4 nucleotides
PP_4912: PP_4912 - DNA topoisomerase IV subunit A, at 5,583,473 to 5,585,731
_4912
PP_4913: PP_4913 - conserved protein of unknown function, at 5,585,745 to 5,586,263
_4913
PP_4914: PP_4914 - putative phytase-like protein, at 5,586,260 to 5,587,285
_4914
PP_4915: PP_4915 - DNA topoisomerase IV subunit B, at 5,587,243 to 5,589,147
_4915
Position (kb) 5585
5586
5587 Strain fitness (log2 ratio) -2
-1
0
1 at 5585.747 kb on - strand at 5585.826 kb on + strand, within PP_4913 at 5585.826 kb on + strand, within PP_4913 at 5585.827 kb on - strand, within PP_4913 at 5585.866 kb on - strand, within PP_4913 at 5585.949 kb on + strand, within PP_4913 at 5585.950 kb on - strand, within PP_4913 at 5586.089 kb on + strand, within PP_4913 at 5586.089 kb on + strand, within PP_4913 at 5586.228 kb on + strand at 5586.229 kb on - strand at 5586.511 kb on + strand, within PP_4914 at 5586.511 kb on + strand, within PP_4914 at 5586.910 kb on + strand, within PP_4914 at 5586.942 kb on + strand, within PP_4914 at 5587.015 kb on + strand, within PP_4914 at 5587.016 kb on - strand, within PP_4914 at 5587.016 kb on - strand, within PP_4914 at 5587.063 kb on + strand, within PP_4914 at 5587.064 kb on - strand, within PP_4914 at 5587.232 kb on + strand at 5587.233 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Temperature shift 10_to_34; with MOPS remove 5,585,747 - -1.2 5,585,826 + PP_4913 0.16 +0.4 5,585,826 + PP_4913 0.16 -2.3 5,585,827 - PP_4913 0.16 -2.2 5,585,866 - PP_4913 0.23 -0.6 5,585,949 + PP_4913 0.39 -1.3 5,585,950 - PP_4913 0.39 +0.0 5,586,089 + PP_4913 0.66 -1.2 5,586,089 + PP_4913 0.66 -0.1 5,586,228 + +0.9 5,586,229 - -1.4 5,586,511 + PP_4914 0.24 +1.0 5,586,511 + PP_4914 0.24 -0.3 5,586,910 + PP_4914 0.63 -0.8 5,586,942 + PP_4914 0.66 +0.4 5,587,015 + PP_4914 0.74 -1.2 5,587,016 - PP_4914 0.74 -0.6 5,587,016 - PP_4914 0.74 -0.5 5,587,063 + PP_4914 0.78 +0.3 5,587,064 - PP_4914 0.78 +1.3 5,587,232 + +0.3 5,587,233 - +0.5
Or see this region's nucleotide sequence