Experiment: D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17595 and BWI76_RS17600 are separated by 96 nucleotides BWI76_RS17600 and BWI76_RS17605 are separated by 228 nucleotides BWI76_RS17605 and BWI76_RS17610 are separated by 314 nucleotides
BWI76_RS17595: BWI76_RS17595 - hypothetical protein, at 3,655,426 to 3,655,953
_RS17595
BWI76_RS17600: BWI76_RS17600 - hypothetical protein, at 3,656,050 to 3,656,535
_RS17600
BWI76_RS17605: BWI76_RS17605 - phosphoenolpyruvate carboxykinase (ATP), at 3,656,764 to 3,658,404
_RS17605
BWI76_RS17610: BWI76_RS17610 - LysR family transcriptional regulator, at 3,658,719 to 3,659,612
_RS17610
Position (kb)
3656
3657
3658
3659 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3655.769 kb on + strand, within BWI76_RS17595 at 3655.828 kb on - strand, within BWI76_RS17595 at 3655.880 kb on + strand, within BWI76_RS17595 at 3655.880 kb on + strand, within BWI76_RS17595 at 3655.887 kb on - strand, within BWI76_RS17595 at 3655.892 kb on - strand, within BWI76_RS17595 at 3655.943 kb on + strand at 3655.951 kb on - strand at 3655.955 kb on + strand at 3656.084 kb on - strand at 3656.226 kb on + strand, within BWI76_RS17600 at 3656.234 kb on + strand, within BWI76_RS17600 at 3656.234 kb on + strand, within BWI76_RS17600 at 3656.234 kb on - strand, within BWI76_RS17600 at 3656.234 kb on - strand, within BWI76_RS17600 at 3656.234 kb on - strand, within BWI76_RS17600 at 3656.239 kb on + strand, within BWI76_RS17600 at 3656.240 kb on + strand, within BWI76_RS17600 at 3656.240 kb on + strand, within BWI76_RS17600 at 3656.242 kb on - strand, within BWI76_RS17600 at 3656.246 kb on + strand, within BWI76_RS17600 at 3656.247 kb on - strand, within BWI76_RS17600 at 3656.248 kb on - strand, within BWI76_RS17600 at 3656.293 kb on - strand, within BWI76_RS17600 at 3656.305 kb on + strand, within BWI76_RS17600 at 3656.315 kb on - strand, within BWI76_RS17600 at 3656.322 kb on - strand, within BWI76_RS17600 at 3656.323 kb on - strand, within BWI76_RS17600 at 3656.328 kb on - strand, within BWI76_RS17600 at 3656.334 kb on - strand, within BWI76_RS17600 at 3656.393 kb on + strand, within BWI76_RS17600 at 3656.431 kb on + strand, within BWI76_RS17600 at 3656.431 kb on + strand, within BWI76_RS17600 at 3656.438 kb on + strand, within BWI76_RS17600 at 3656.439 kb on - strand, within BWI76_RS17600 at 3656.439 kb on - strand, within BWI76_RS17600 at 3656.439 kb on - strand, within BWI76_RS17600 at 3656.439 kb on - strand, within BWI76_RS17600 at 3656.439 kb on - strand, within BWI76_RS17600 at 3656.439 kb on - strand, within BWI76_RS17600 at 3656.448 kb on + strand, within BWI76_RS17600 at 3656.469 kb on + strand, within BWI76_RS17600 at 3656.499 kb on + strand at 3656.509 kb on - strand at 3656.522 kb on - strand at 3656.522 kb on - strand at 3656.522 kb on - strand at 3656.571 kb on + strand at 3656.577 kb on - strand at 3656.582 kb on - strand at 3656.587 kb on + strand at 3656.587 kb on + strand at 3656.587 kb on + strand at 3656.587 kb on + strand at 3656.594 kb on - strand at 3656.594 kb on - strand at 3656.595 kb on - strand at 3656.595 kb on - strand at 3656.596 kb on + strand at 3656.604 kb on - strand at 3656.604 kb on - strand at 3656.606 kb on + strand at 3656.640 kb on + strand at 3656.642 kb on + strand at 3656.648 kb on - strand at 3656.675 kb on + strand at 3656.686 kb on + strand at 3656.686 kb on + strand at 3656.692 kb on - strand at 3656.692 kb on - strand at 3656.698 kb on + strand at 3656.698 kb on + strand at 3656.745 kb on - strand at 3656.780 kb on + strand at 3656.800 kb on + strand at 3656.849 kb on - strand at 3656.929 kb on - strand, within BWI76_RS17605 at 3657.054 kb on + strand, within BWI76_RS17605 at 3657.062 kb on - strand, within BWI76_RS17605 at 3657.086 kb on + strand, within BWI76_RS17605 at 3657.094 kb on - strand, within BWI76_RS17605 at 3657.094 kb on - strand, within BWI76_RS17605 at 3657.218 kb on - strand, within BWI76_RS17605 at 3657.218 kb on - strand, within BWI76_RS17605 at 3657.339 kb on + strand, within BWI76_RS17605 at 3657.458 kb on + strand, within BWI76_RS17605 at 3657.459 kb on - strand, within BWI76_RS17605 at 3657.466 kb on - strand, within BWI76_RS17605 at 3657.587 kb on + strand, within BWI76_RS17605 at 3657.733 kb on - strand, within BWI76_RS17605 at 3657.825 kb on + strand, within BWI76_RS17605 at 3657.834 kb on - strand, within BWI76_RS17605 at 3657.848 kb on + strand, within BWI76_RS17605 at 3657.856 kb on + strand, within BWI76_RS17605 at 3657.856 kb on + strand, within BWI76_RS17605 at 3657.856 kb on - strand, within BWI76_RS17605 at 3657.880 kb on + strand, within BWI76_RS17605 at 3657.893 kb on + strand, within BWI76_RS17605 at 3657.895 kb on + strand, within BWI76_RS17605 at 3657.903 kb on - strand, within BWI76_RS17605 at 3658.071 kb on + strand, within BWI76_RS17605 at 3658.180 kb on - strand, within BWI76_RS17605 at 3658.180 kb on - strand, within BWI76_RS17605 at 3658.233 kb on - strand, within BWI76_RS17605 at 3658.248 kb on + strand at 3658.252 kb on + strand at 3658.290 kb on - strand at 3658.295 kb on - strand at 3658.295 kb on - strand at 3658.318 kb on + strand at 3658.349 kb on - strand at 3658.351 kb on + strand at 3658.353 kb on + strand at 3658.353 kb on + strand at 3658.353 kb on + strand at 3658.353 kb on + strand at 3658.356 kb on - strand at 3658.356 kb on - strand at 3658.359 kb on - strand at 3658.359 kb on - strand at 3658.359 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.361 kb on - strand at 3658.396 kb on - strand at 3658.409 kb on - strand at 3658.419 kb on + strand at 3658.520 kb on + strand at 3658.525 kb on + strand at 3658.525 kb on + strand at 3658.525 kb on + strand at 3658.528 kb on - strand at 3658.528 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.533 kb on - strand at 3658.554 kb on + strand at 3658.554 kb on + strand at 3658.554 kb on + strand at 3658.560 kb on + strand at 3658.562 kb on - strand at 3658.562 kb on - strand at 3658.562 kb on - strand at 3658.566 kb on + strand at 3658.569 kb on - strand at 3658.569 kb on - strand at 3658.573 kb on + strand at 3658.573 kb on + strand at 3658.574 kb on - strand at 3658.581 kb on - strand at 3658.589 kb on - strand at 3658.589 kb on - strand at 3658.589 kb on - strand at 3658.601 kb on + strand at 3658.607 kb on + strand at 3658.607 kb on + strand at 3658.615 kb on - strand at 3658.641 kb on - strand at 3658.641 kb on - strand at 3658.660 kb on + strand at 3658.668 kb on + strand at 3658.668 kb on - strand at 3658.703 kb on + strand at 3658.709 kb on - strand at 3658.714 kb on + strand at 3658.753 kb on + strand at 3658.755 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.762 kb on + strand at 3658.770 kb on - strand at 3658.770 kb on - strand at 3658.770 kb on - strand at 3658.770 kb on - strand at 3658.770 kb on - strand at 3658.772 kb on + strand at 3658.772 kb on + strand at 3658.772 kb on + strand at 3658.772 kb on + strand at 3658.780 kb on + strand at 3658.780 kb on + strand at 3658.788 kb on - strand at 3658.845 kb on + strand, within BWI76_RS17610 at 3658.845 kb on + strand, within BWI76_RS17610 at 3658.845 kb on + strand, within BWI76_RS17610 at 3658.869 kb on + strand, within BWI76_RS17610 at 3658.874 kb on + strand, within BWI76_RS17610 at 3658.958 kb on - strand, within BWI76_RS17610 at 3659.102 kb on + strand, within BWI76_RS17610 at 3659.108 kb on + strand, within BWI76_RS17610 at 3659.118 kb on + strand, within BWI76_RS17610 at 3659.130 kb on - strand, within BWI76_RS17610 at 3659.167 kb on + strand, within BWI76_RS17610 at 3659.183 kb on + strand, within BWI76_RS17610 at 3659.215 kb on + strand at 3659.220 kb on + strand, within BWI76_RS17610 at 3659.247 kb on + strand, within BWI76_RS17610 at 3659.255 kb on - strand, within BWI76_RS17610 at 3659.255 kb on - strand, within BWI76_RS17610 at 3659.257 kb on - strand, within BWI76_RS17610 at 3659.283 kb on + strand, within BWI76_RS17610 at 3659.314 kb on + strand, within BWI76_RS17610 at 3659.323 kb on - strand, within BWI76_RS17610 at 3659.339 kb on + strand, within BWI76_RS17610 at 3659.340 kb on + strand, within BWI76_RS17610 at 3659.348 kb on - strand, within BWI76_RS17610 at 3659.370 kb on + strand, within BWI76_RS17610 at 3659.385 kb on - strand, within BWI76_RS17610
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) and no Nitrogen; normal Wolfe's minerals with nitroloacetic; 8 days remove 3,655,769 + BWI76_RS17595 0.65 +0.5 3,655,828 - BWI76_RS17595 0.76 -0.2 3,655,880 + BWI76_RS17595 0.86 -0.9 3,655,880 + BWI76_RS17595 0.86 +0.4 3,655,887 - BWI76_RS17595 0.87 -0.2 3,655,892 - BWI76_RS17595 0.88 +0.8 3,655,943 + +0.4 3,655,951 - -0.8 3,655,955 + -0.9 3,656,084 - -0.1 3,656,226 + BWI76_RS17600 0.36 -0.3 3,656,234 + BWI76_RS17600 0.38 +0.6 3,656,234 + BWI76_RS17600 0.38 -0.1 3,656,234 - BWI76_RS17600 0.38 -0.7 3,656,234 - BWI76_RS17600 0.38 +0.1 3,656,234 - BWI76_RS17600 0.38 -0.6 3,656,239 + BWI76_RS17600 0.39 +0.2 3,656,240 + BWI76_RS17600 0.39 +0.1 3,656,240 + BWI76_RS17600 0.39 -2.0 3,656,242 - BWI76_RS17600 0.40 -0.0 3,656,246 + BWI76_RS17600 0.40 -0.9 3,656,247 - BWI76_RS17600 0.41 -0.5 3,656,248 - BWI76_RS17600 0.41 +0.3 3,656,293 - BWI76_RS17600 0.50 -1.1 3,656,305 + BWI76_RS17600 0.52 +2.2 3,656,315 - BWI76_RS17600 0.55 -0.6 3,656,322 - BWI76_RS17600 0.56 -0.5 3,656,323 - BWI76_RS17600 0.56 +0.4 3,656,328 - BWI76_RS17600 0.57 +0.9 3,656,334 - BWI76_RS17600 0.58 +1.0 3,656,393 + BWI76_RS17600 0.71 -0.3 3,656,431 + BWI76_RS17600 0.78 -0.2 3,656,431 + BWI76_RS17600 0.78 -0.0 3,656,438 + BWI76_RS17600 0.80 -0.0 3,656,439 - BWI76_RS17600 0.80 +0.3 3,656,439 - BWI76_RS17600 0.80 +0.3 3,656,439 - BWI76_RS17600 0.80 -0.5 3,656,439 - BWI76_RS17600 0.80 -0.4 3,656,439 - BWI76_RS17600 0.80 -1.7 3,656,439 - BWI76_RS17600 0.80 +2.3 3,656,448 + BWI76_RS17600 0.82 -0.2 3,656,469 + BWI76_RS17600 0.86 -0.4 3,656,499 + -1.7 3,656,509 - -1.9 3,656,522 - +0.6 3,656,522 - +0.5 3,656,522 - +0.3 3,656,571 + -0.6 3,656,577 - +1.1 3,656,582 - -0.1 3,656,587 + +0.2 3,656,587 + -0.2 3,656,587 + +0.1 3,656,587 + +0.4 3,656,594 - +0.9 3,656,594 - +0.1 3,656,595 - +0.0 3,656,595 - -0.4 3,656,596 + -0.5 3,656,604 - +0.1 3,656,604 - -0.3 3,656,606 + +0.2 3,656,640 + +0.2 3,656,642 + -0.4 3,656,648 - +1.0 3,656,675 + +0.2 3,656,686 + +0.0 3,656,686 + +0.4 3,656,692 - +0.3 3,656,692 - +1.6 3,656,698 + +0.1 3,656,698 + -0.2 3,656,745 - +0.7 3,656,780 + +0.2 3,656,800 + -0.0 3,656,849 - +1.3 3,656,929 - BWI76_RS17605 0.10 +0.5 3,657,054 + BWI76_RS17605 0.18 +0.8 3,657,062 - BWI76_RS17605 0.18 +0.3 3,657,086 + BWI76_RS17605 0.20 +0.2 3,657,094 - BWI76_RS17605 0.20 +0.6 3,657,094 - BWI76_RS17605 0.20 +1.2 3,657,218 - BWI76_RS17605 0.28 +0.6 3,657,218 - BWI76_RS17605 0.28 +0.6 3,657,339 + BWI76_RS17605 0.35 +1.3 3,657,458 + BWI76_RS17605 0.42 +0.6 3,657,459 - BWI76_RS17605 0.42 +0.9 3,657,466 - BWI76_RS17605 0.43 +0.6 3,657,587 + BWI76_RS17605 0.50 -0.2 3,657,733 - BWI76_RS17605 0.59 +1.0 3,657,825 + BWI76_RS17605 0.65 +1.3 3,657,834 - BWI76_RS17605 0.65 -0.4 3,657,848 + BWI76_RS17605 0.66 +0.6 3,657,856 + BWI76_RS17605 0.67 +0.3 3,657,856 + BWI76_RS17605 0.67 +0.6 3,657,856 - BWI76_RS17605 0.67 +0.7 3,657,880 + BWI76_RS17605 0.68 +0.6 3,657,893 + BWI76_RS17605 0.69 +0.7 3,657,895 + BWI76_RS17605 0.69 +0.8 3,657,903 - BWI76_RS17605 0.69 +0.7 3,658,071 + BWI76_RS17605 0.80 +0.8 3,658,180 - BWI76_RS17605 0.86 +1.0 3,658,180 - BWI76_RS17605 0.86 +1.2 3,658,233 - BWI76_RS17605 0.90 +0.8 3,658,248 + +1.1 3,658,252 + +0.5 3,658,290 - +0.8 3,658,295 - +0.4 3,658,295 - +0.6 3,658,318 + +0.1 3,658,349 - +0.8 3,658,351 + +0.8 3,658,353 + -1.5 3,658,353 + +0.1 3,658,353 + -0.1 3,658,353 + +0.4 3,658,356 - +0.4 3,658,356 - +1.0 3,658,359 - +1.5 3,658,359 - +1.3 3,658,359 - +0.6 3,658,361 - +0.8 3,658,361 - +0.9 3,658,361 - +0.1 3,658,361 - +2.6 3,658,361 - +0.6 3,658,361 - +1.3 3,658,361 - +0.5 3,658,361 - +1.0 3,658,361 - +1.0 3,658,361 - +0.8 3,658,361 - +0.5 3,658,361 - +0.8 3,658,361 - +0.7 3,658,361 - +0.8 3,658,361 - +0.5 3,658,361 - +0.9 3,658,361 - +0.9 3,658,361 - +0.7 3,658,361 - +2.1 3,658,361 - +1.0 3,658,361 - +0.7 3,658,361 - +1.3 3,658,361 - +0.7 3,658,361 - +0.8 3,658,361 - +3.2 3,658,361 - +1.0 3,658,361 - +0.6 3,658,361 - +0.9 3,658,361 - +1.1 3,658,396 - +1.1 3,658,409 - +0.8 3,658,419 + -0.0 3,658,520 + +0.6 3,658,525 + +0.4 3,658,525 + +0.6 3,658,525 + +0.0 3,658,528 - +1.1 3,658,528 - +0.8 3,658,533 - +0.4 3,658,533 - +1.1 3,658,533 - +0.4 3,658,533 - +1.1 3,658,533 - +0.6 3,658,533 - +0.6 3,658,533 - -0.1 3,658,533 - +0.9 3,658,533 - +1.0 3,658,533 - +0.9 3,658,554 + +0.2 3,658,554 + +0.3 3,658,554 + +0.2 3,658,560 + -1.0 3,658,562 - +0.6 3,658,562 - -0.2 3,658,562 - +0.1 3,658,566 + +1.6 3,658,569 - +1.0 3,658,569 - +0.9 3,658,573 + -0.2 3,658,573 + -0.7 3,658,574 - +0.3 3,658,581 - +0.2 3,658,589 - +0.8 3,658,589 - +0.8 3,658,589 - -0.0 3,658,601 + -0.8 3,658,607 + +0.3 3,658,607 + -1.1 3,658,615 - +0.6 3,658,641 - +0.3 3,658,641 - +0.7 3,658,660 + -0.2 3,658,668 + +0.5 3,658,668 - +1.9 3,658,703 + -0.5 3,658,709 - +1.2 3,658,714 + +0.5 3,658,753 + +1.3 3,658,755 + +1.5 3,658,762 + +0.7 3,658,762 + +0.6 3,658,762 + +0.0 3,658,762 + +0.9 3,658,762 + -0.4 3,658,762 + +1.2 3,658,762 + +1.2 3,658,762 + +0.8 3,658,762 + +2.2 3,658,762 + +0.4 3,658,762 + +0.0 3,658,762 + +0.6 3,658,762 + -0.1 3,658,762 + +0.7 3,658,762 + -0.2 3,658,762 + +0.2 3,658,762 + -0.5 3,658,762 + +0.6 3,658,770 - -0.4 3,658,770 - +2.1 3,658,770 - +1.0 3,658,770 - -0.0 3,658,770 - +0.2 3,658,772 + +0.2 3,658,772 + -0.6 3,658,772 + +1.2 3,658,772 + -0.2 3,658,780 + +0.4 3,658,780 + +0.1 3,658,788 - +0.8 3,658,845 + BWI76_RS17610 0.14 +0.9 3,658,845 + BWI76_RS17610 0.14 +0.1 3,658,845 + BWI76_RS17610 0.14 +0.3 3,658,869 + BWI76_RS17610 0.17 +0.7 3,658,874 + BWI76_RS17610 0.17 +0.3 3,658,958 - BWI76_RS17610 0.27 +0.3 3,659,102 + BWI76_RS17610 0.43 +1.1 3,659,108 + BWI76_RS17610 0.44 +0.9 3,659,118 + BWI76_RS17610 0.45 +1.4 3,659,130 - BWI76_RS17610 0.46 +1.6 3,659,167 + BWI76_RS17610 0.50 +0.3 3,659,183 + BWI76_RS17610 0.52 +0.6 3,659,215 + -0.2 3,659,220 + BWI76_RS17610 0.56 +0.6 3,659,247 + BWI76_RS17610 0.59 +0.5 3,659,255 - BWI76_RS17610 0.60 -0.7 3,659,255 - BWI76_RS17610 0.60 +1.1 3,659,257 - BWI76_RS17610 0.60 -1.4 3,659,283 + BWI76_RS17610 0.63 +0.6 3,659,314 + BWI76_RS17610 0.67 +0.3 3,659,323 - BWI76_RS17610 0.68 +0.9 3,659,339 + BWI76_RS17610 0.69 +1.1 3,659,340 + BWI76_RS17610 0.69 +0.1 3,659,348 - BWI76_RS17610 0.70 +1.5 3,659,370 + BWI76_RS17610 0.73 +0.7 3,659,385 - BWI76_RS17610 0.74 +1.4
Or see this region's nucleotide sequence