Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS21770

Experiment: 4-Hydroxybenzoic Acid (C) 1.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS21760 and PFLU_RS21765 overlap by 11 nucleotidesPFLU_RS21765 and PFLU_RS21770 are separated by 10 nucleotidesPFLU_RS21770 and PFLU_RS21775 overlap by 8 nucleotides PFLU_RS21760: PFLU_RS21760 - flagellar protein export ATPase FliI, at 4,898,823 to 4,900,181 _RS21760 PFLU_RS21765: PFLU_RS21765 - flagellar assembly protein FliH, at 4,900,171 to 4,900,935 _RS21765 PFLU_RS21770: PFLU_RS21770 - flagellar motor switch protein FliG, at 4,900,946 to 4,901,962 _RS21770 PFLU_RS21775: PFLU_RS21775 - flagellar basal body M-ring protein FliF, at 4,901,955 to 4,903,736 _RS21775 Position (kb) 4900 4901 4902Strain fitness (log2 ratio) -1 0 1 2 3 4at 4900.003 kb on + strand, within PFLU_RS21760at 4900.003 kb on + strand, within PFLU_RS21760at 4900.003 kb on + strand, within PFLU_RS21760at 4900.003 kb on + strand, within PFLU_RS21760at 4900.003 kb on + strand, within PFLU_RS21760at 4900.003 kb on + strand, within PFLU_RS21760at 4900.004 kb on - strand, within PFLU_RS21760at 4900.004 kb on - strand, within PFLU_RS21760at 4900.004 kb on - strand, within PFLU_RS21760at 4900.004 kb on - strand, within PFLU_RS21760at 4900.004 kb on - strand, within PFLU_RS21760at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.424 kb on + strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.425 kb on - strand, within PFLU_RS21765at 4900.724 kb on + strand, within PFLU_RS21765at 4900.724 kb on + strand, within PFLU_RS21765at 4900.724 kb on + strand, within PFLU_RS21765at 4900.724 kb on + strand, within PFLU_RS21765at 4900.725 kb on - strand, within PFLU_RS21765at 4900.725 kb on - strand, within PFLU_RS21765at 4900.725 kb on - strand, within PFLU_RS21765at 4900.725 kb on - strand, within PFLU_RS21765at 4900.725 kb on - strand, within PFLU_RS21765at 4900.725 kb on - strand, within PFLU_RS21765at 4900.947 kb on + strandat 4900.947 kb on + strandat 4900.947 kb on + strandat 4900.947 kb on + strandat 4900.948 kb on - strandat 4900.948 kb on - strandat 4900.948 kb on - strandat 4900.948 kb on - strandat 4901.341 kb on + strand, within PFLU_RS21770at 4901.494 kb on + strand, within PFLU_RS21770at 4901.494 kb on + strand, within PFLU_RS21770at 4901.495 kb on - strand, within PFLU_RS21770at 4901.495 kb on - strand, within PFLU_RS21770at 4901.495 kb on - strand, within PFLU_RS21770at 4901.542 kb on - strand, within PFLU_RS21770at 4901.542 kb on - strand, within PFLU_RS21770at 4901.542 kb on - strand, within PFLU_RS21770at 4901.542 kb on - strand, within PFLU_RS21770at 4901.542 kb on - strand, within PFLU_RS21770at 4901.542 kb on - strand, within PFLU_RS21770at 4901.578 kb on - strand, within PFLU_RS21770at 4901.578 kb on - strand, within PFLU_RS21770at 4901.656 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.718 kb on + strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.719 kb on - strand, within PFLU_RS21770at 4901.933 kb on - strandat 4902.049 kb on + strandat 4902.049 kb on + strandat 4902.050 kb on - strandat 4902.238 kb on - strand, within PFLU_RS21775at 4902.298 kb on + strand, within PFLU_RS21775at 4902.298 kb on + strand, within PFLU_RS21775at 4902.299 kb on - strand, within PFLU_RS21775at 4902.299 kb on - strand, within PFLU_RS21775at 4902.587 kb on + strand, within PFLU_RS21775at 4902.587 kb on + strand, within PFLU_RS21775at 4902.587 kb on + strand, within PFLU_RS21775at 4902.588 kb on - strand, within PFLU_RS21775at 4902.588 kb on - strand, within PFLU_RS21775at 4902.588 kb on - strand, within PFLU_RS21775at 4902.588 kb on - strand, within PFLU_RS21775at 4902.588 kb on - strand, within PFLU_RS21775at 4902.910 kb on + strand, within PFLU_RS21775at 4902.910 kb on + strand, within PFLU_RS21775at 4902.910 kb on + strand, within PFLU_RS21775at 4902.911 kb on - strand, within PFLU_RS21775at 4902.911 kb on - strand, within PFLU_RS21775at 4902.911 kb on - strand, within PFLU_RS21775at 4902.944 kb on + strand, within PFLU_RS21775at 4902.944 kb on + strand, within PFLU_RS21775at 4902.944 kb on + strand, within PFLU_RS21775at 4902.944 kb on + strand, within PFLU_RS21775at 4902.944 kb on + strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775at 4902.945 kb on - strand, within PFLU_RS21775

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) 1.5 mM
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4,900,003 + PFLU_RS21760 0.87 -0.2
4,900,003 + PFLU_RS21760 0.87 +0.4
4,900,003 + PFLU_RS21760 0.87 +2.1
4,900,003 + PFLU_RS21760 0.87 +0.3
4,900,003 + PFLU_RS21760 0.87 +2.3
4,900,003 + PFLU_RS21760 0.87 +3.9
4,900,004 - PFLU_RS21760 0.87 +0.4
4,900,004 - PFLU_RS21760 0.87 +1.1
4,900,004 - PFLU_RS21760 0.87 +0.8
4,900,004 - PFLU_RS21760 0.87 +1.9
4,900,004 - PFLU_RS21760 0.87 +1.7
4,900,424 + PFLU_RS21765 0.33 +1.3
4,900,424 + PFLU_RS21765 0.33 +1.5
4,900,424 + PFLU_RS21765 0.33 +2.2
4,900,424 + PFLU_RS21765 0.33 +1.4
4,900,424 + PFLU_RS21765 0.33 +1.4
4,900,424 + PFLU_RS21765 0.33 +0.6
4,900,424 + PFLU_RS21765 0.33 +3.2
4,900,424 + PFLU_RS21765 0.33 -0.0
4,900,424 + PFLU_RS21765 0.33 +0.1
4,900,424 + PFLU_RS21765 0.33 +0.4
4,900,425 - PFLU_RS21765 0.33 +0.8
4,900,425 - PFLU_RS21765 0.33 +0.6
4,900,425 - PFLU_RS21765 0.33 +1.1
4,900,425 - PFLU_RS21765 0.33 +1.3
4,900,425 - PFLU_RS21765 0.33 +1.5
4,900,425 - PFLU_RS21765 0.33 +2.7
4,900,425 - PFLU_RS21765 0.33 +1.5
4,900,425 - PFLU_RS21765 0.33 +1.1
4,900,425 - PFLU_RS21765 0.33 +2.0
4,900,724 + PFLU_RS21765 0.72 +0.7
4,900,724 + PFLU_RS21765 0.72 +0.6
4,900,724 + PFLU_RS21765 0.72 +1.0
4,900,724 + PFLU_RS21765 0.72 +0.6
4,900,725 - PFLU_RS21765 0.72 +1.0
4,900,725 - PFLU_RS21765 0.72 +1.4
4,900,725 - PFLU_RS21765 0.72 +2.5
4,900,725 - PFLU_RS21765 0.72 +0.3
4,900,725 - PFLU_RS21765 0.72 +0.2
4,900,725 - PFLU_RS21765 0.72 +0.4
4,900,947 + +1.8
4,900,947 + +0.4
4,900,947 + +0.9
4,900,947 + +0.5
4,900,948 - -1.2
4,900,948 - +0.1
4,900,948 - +0.5
4,900,948 - -0.6
4,901,341 + PFLU_RS21770 0.39 +0.1
4,901,494 + PFLU_RS21770 0.54 +1.0
4,901,494 + PFLU_RS21770 0.54 +0.0
4,901,495 - PFLU_RS21770 0.54 +0.2
4,901,495 - PFLU_RS21770 0.54 +1.9
4,901,495 - PFLU_RS21770 0.54 +1.0
4,901,542 - PFLU_RS21770 0.59 -0.2
4,901,542 - PFLU_RS21770 0.59 +0.6
4,901,542 - PFLU_RS21770 0.59 +0.4
4,901,542 - PFLU_RS21770 0.59 +1.0
4,901,542 - PFLU_RS21770 0.59 +1.3
4,901,542 - PFLU_RS21770 0.59 +1.4
4,901,578 - PFLU_RS21770 0.62 +1.2
4,901,578 - PFLU_RS21770 0.62 +1.0
4,901,656 + PFLU_RS21770 0.70 +0.5
4,901,718 + PFLU_RS21770 0.76 +0.8
4,901,718 + PFLU_RS21770 0.76 +0.9
4,901,718 + PFLU_RS21770 0.76 +1.1
4,901,718 + PFLU_RS21770 0.76 +0.7
4,901,718 + PFLU_RS21770 0.76 +0.9
4,901,718 + PFLU_RS21770 0.76 -0.0
4,901,718 + PFLU_RS21770 0.76 +2.2
4,901,718 + PFLU_RS21770 0.76 +0.5
4,901,718 + PFLU_RS21770 0.76 +0.9
4,901,718 + PFLU_RS21770 0.76 +0.4
4,901,718 + PFLU_RS21770 0.76 +0.6
4,901,718 + PFLU_RS21770 0.76 +0.5
4,901,718 + PFLU_RS21770 0.76 +0.6
4,901,718 + PFLU_RS21770 0.76 -1.3
4,901,718 + PFLU_RS21770 0.76 +1.4
4,901,718 + PFLU_RS21770 0.76 -0.4
4,901,718 + PFLU_RS21770 0.76 +0.7
4,901,718 + PFLU_RS21770 0.76 +0.3
4,901,719 - PFLU_RS21770 0.76 +0.0
4,901,719 - PFLU_RS21770 0.76 -0.3
4,901,719 - PFLU_RS21770 0.76 +0.1
4,901,719 - PFLU_RS21770 0.76 -0.3
4,901,719 - PFLU_RS21770 0.76 +1.7
4,901,719 - PFLU_RS21770 0.76 -0.9
4,901,719 - PFLU_RS21770 0.76 +0.4
4,901,719 - PFLU_RS21770 0.76 -0.5
4,901,719 - PFLU_RS21770 0.76 -0.2
4,901,933 - -0.2
4,902,049 + -0.8
4,902,049 + +1.6
4,902,050 - +2.1
4,902,238 - PFLU_RS21775 0.16 +1.7
4,902,298 + PFLU_RS21775 0.19 +2.0
4,902,298 + PFLU_RS21775 0.19 +0.7
4,902,299 - PFLU_RS21775 0.19 -0.8
4,902,299 - PFLU_RS21775 0.19 +1.1
4,902,587 + PFLU_RS21775 0.35 -0.9
4,902,587 + PFLU_RS21775 0.35 +0.4
4,902,587 + PFLU_RS21775 0.35 +0.6
4,902,588 - PFLU_RS21775 0.36 +1.4
4,902,588 - PFLU_RS21775 0.36 +0.9
4,902,588 - PFLU_RS21775 0.36 +0.9
4,902,588 - PFLU_RS21775 0.36 +1.2
4,902,588 - PFLU_RS21775 0.36 +1.4
4,902,910 + PFLU_RS21775 0.54 +2.6
4,902,910 + PFLU_RS21775 0.54 -0.3
4,902,910 + PFLU_RS21775 0.54 +1.7
4,902,911 - PFLU_RS21775 0.54 +0.3
4,902,911 - PFLU_RS21775 0.54 +1.7
4,902,911 - PFLU_RS21775 0.54 +1.0
4,902,944 + PFLU_RS21775 0.55 +1.8
4,902,944 + PFLU_RS21775 0.55 +1.6
4,902,944 + PFLU_RS21775 0.55 +0.1
4,902,944 + PFLU_RS21775 0.55 +2.1
4,902,944 + PFLU_RS21775 0.55 +0.0
4,902,945 - PFLU_RS21775 0.56 +0.9
4,902,945 - PFLU_RS21775 0.56 +1.4
4,902,945 - PFLU_RS21775 0.56 +2.2
4,902,945 - PFLU_RS21775 0.56 -0.1
4,902,945 - PFLU_RS21775 0.56 +1.3
4,902,945 - PFLU_RS21775 0.56 +1.2
4,902,945 - PFLU_RS21775 0.56 -0.2
4,902,945 - PFLU_RS21775 0.56 +1.2
4,902,945 - PFLU_RS21775 0.56 -0.0

Or see this region's nucleotide sequence