Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS14420

Experiment: 4-Hydroxybenzoic Acid (C) 1.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS14415 and PFLU_RS14420 are separated by 724 nucleotidesPFLU_RS14420 and PFLU_RS14425 are separated by 73 nucleotidesPFLU_RS14425 and PFLU_RS14430 are separated by 43 nucleotides PFLU_RS14415: PFLU_RS14415 - magnesium-translocating P-type ATPase, at 3,228,675 to 3,231,389 _RS14415 PFLU_RS14420: PFLU_RS14420 - chemotactic transduction protein ChpE, at 3,232,114 to 3,232,725 _RS14420 PFLU_RS14425: PFLU_RS14425 - AraC family transcriptional regulator, at 3,232,799 to 3,233,590 _RS14425 PFLU_RS14430: PFLU_RS14430 - arsenate reductase ArsC, at 3,233,634 to 3,234,104 _RS14430 Position (kb) 3232 3233Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3231.129 kb on + strandat 3231.129 kb on + strandat 3231.129 kb on + strandat 3231.129 kb on + strandat 3231.129 kb on + strandat 3231.130 kb on - strandat 3231.130 kb on - strandat 3231.130 kb on - strandat 3231.130 kb on - strandat 3231.130 kb on - strandat 3231.130 kb on - strandat 3231.130 kb on - strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.133 kb on + strandat 3231.134 kb on - strandat 3231.134 kb on - strandat 3231.134 kb on - strandat 3231.134 kb on - strandat 3231.134 kb on - strandat 3231.134 kb on - strandat 3231.208 kb on + strandat 3231.208 kb on + strandat 3231.209 kb on - strandat 3231.434 kb on - strandat 3231.437 kb on + strandat 3231.504 kb on + strandat 3231.504 kb on + strandat 3231.504 kb on + strandat 3231.504 kb on + strandat 3231.504 kb on + strandat 3231.504 kb on + strandat 3231.504 kb on + strandat 3231.505 kb on - strandat 3231.505 kb on - strandat 3231.505 kb on - strandat 3231.509 kb on - strandat 3231.549 kb on + strandat 3231.550 kb on - strandat 3231.561 kb on - strandat 3231.760 kb on + strandat 3231.760 kb on + strandat 3231.761 kb on - strandat 3231.761 kb on - strandat 3231.761 kb on - strandat 3231.761 kb on - strandat 3231.761 kb on - strandat 3231.761 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.797 kb on - strandat 3231.837 kb on - strandat 3231.949 kb on - strandat 3231.949 kb on - strandat 3231.949 kb on - strandat 3231.949 kb on - strandat 3231.949 kb on - strandat 3231.949 kb on - strandat 3231.949 kb on - strandat 3231.949 kb on - strandat 3232.049 kb on + strandat 3232.105 kb on + strandat 3232.105 kb on + strandat 3232.105 kb on + strandat 3232.106 kb on - strandat 3232.106 kb on - strandat 3232.106 kb on - strandat 3232.107 kb on + strandat 3232.108 kb on - strandat 3232.109 kb on + strandat 3232.118 kb on - strandat 3232.118 kb on - strandat 3232.145 kb on + strandat 3232.146 kb on - strandat 3232.146 kb on - strandat 3232.153 kb on + strandat 3232.153 kb on + strandat 3232.153 kb on + strandat 3232.154 kb on - strandat 3232.156 kb on + strandat 3232.172 kb on + strandat 3232.349 kb on + strand, within PFLU_RS14420at 3232.357 kb on - strand, within PFLU_RS14420at 3232.357 kb on - strand, within PFLU_RS14420at 3232.487 kb on + strand, within PFLU_RS14420at 3232.487 kb on + strand, within PFLU_RS14420at 3232.488 kb on - strand, within PFLU_RS14420at 3232.488 kb on - strand, within PFLU_RS14420at 3232.633 kb on + strand, within PFLU_RS14420at 3232.696 kb on + strandat 3232.707 kb on - strandat 3232.765 kb on - strandat 3232.955 kb on + strand, within PFLU_RS14425at 3232.955 kb on + strand, within PFLU_RS14425at 3232.956 kb on - strand, within PFLU_RS14425at 3232.956 kb on - strand, within PFLU_RS14425at 3232.956 kb on - strand, within PFLU_RS14425at 3232.956 kb on - strand, within PFLU_RS14425at 3232.956 kb on - strand, within PFLU_RS14425at 3232.956 kb on - strand, within PFLU_RS14425at 3233.044 kb on + strand, within PFLU_RS14425at 3233.044 kb on + strand, within PFLU_RS14425at 3233.045 kb on - strand, within PFLU_RS14425at 3233.045 kb on - strand, within PFLU_RS14425at 3233.045 kb on - strand, within PFLU_RS14425at 3233.045 kb on - strand, within PFLU_RS14425at 3233.045 kb on - strand, within PFLU_RS14425at 3233.237 kb on + strand, within PFLU_RS14425at 3233.237 kb on + strand, within PFLU_RS14425at 3233.303 kb on + strand, within PFLU_RS14425at 3233.303 kb on + strand, within PFLU_RS14425at 3233.303 kb on + strand, within PFLU_RS14425at 3233.344 kb on - strand, within PFLU_RS14425at 3233.344 kb on - strand, within PFLU_RS14425at 3233.409 kb on + strand, within PFLU_RS14425at 3233.409 kb on + strand, within PFLU_RS14425at 3233.409 kb on + strand, within PFLU_RS14425at 3233.409 kb on + strand, within PFLU_RS14425at 3233.409 kb on + strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.410 kb on - strand, within PFLU_RS14425at 3233.416 kb on + strand, within PFLU_RS14425at 3233.417 kb on - strand, within PFLU_RS14425at 3233.635 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 4-Hydroxybenzoic Acid (C) 1.5 mM
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3,231,129 + +2.4
3,231,129 + -1.6
3,231,129 + -0.8
3,231,129 + -0.8
3,231,129 + +1.4
3,231,130 - +0.3
3,231,130 - +0.9
3,231,130 - +0.0
3,231,130 - -1.0
3,231,130 - +1.0
3,231,130 - +0.2
3,231,130 - -0.4
3,231,133 + -0.7
3,231,133 + -0.7
3,231,133 + +0.8
3,231,133 + +0.1
3,231,133 + +0.0
3,231,133 + +3.0
3,231,133 + +1.4
3,231,133 + -0.2
3,231,133 + +1.5
3,231,133 + +2.1
3,231,133 + +0.9
3,231,133 + -0.4
3,231,133 + -1.4
3,231,133 + -0.7
3,231,134 - -0.2
3,231,134 - -0.7
3,231,134 - -0.8
3,231,134 - -0.2
3,231,134 - +0.5
3,231,134 - -0.4
3,231,208 + +0.0
3,231,208 + -0.6
3,231,209 - +1.4
3,231,434 - -0.2
3,231,437 + -0.7
3,231,504 + +2.1
3,231,504 + -1.8
3,231,504 + -0.6
3,231,504 + +0.6
3,231,504 + +2.0
3,231,504 + +0.4
3,231,504 + -0.3
3,231,505 - -2.2
3,231,505 - -0.5
3,231,505 - -1.8
3,231,509 - -0.2
3,231,549 + +0.4
3,231,550 - -0.2
3,231,561 - +2.0
3,231,760 + +1.1
3,231,760 + -1.2
3,231,761 - -0.4
3,231,761 - +0.5
3,231,761 - -2.1
3,231,761 - -0.2
3,231,761 - -0.0
3,231,761 - +1.0
3,231,797 - +0.1
3,231,797 - -0.8
3,231,797 - -1.8
3,231,797 - +0.9
3,231,797 - +0.4
3,231,797 - +0.5
3,231,797 - +0.7
3,231,797 - -1.0
3,231,797 - -0.4
3,231,797 - -1.1
3,231,797 - -1.9
3,231,837 - -0.2
3,231,949 - +2.6
3,231,949 - +0.5
3,231,949 - -0.5
3,231,949 - -0.5
3,231,949 - -2.3
3,231,949 - -0.9
3,231,949 - +2.4
3,231,949 - +2.1
3,232,049 + -0.9
3,232,105 + -0.4
3,232,105 + +1.1
3,232,105 + -0.2
3,232,106 - -1.4
3,232,106 - -0.4
3,232,106 - -2.7
3,232,107 + +0.1
3,232,108 - +2.0
3,232,109 + -2.9
3,232,118 - +3.0
3,232,118 - -0.6
3,232,145 + +1.4
3,232,146 - +0.4
3,232,146 - -0.5
3,232,153 + -0.9
3,232,153 + -1.9
3,232,153 + +1.0
3,232,154 - -1.5
3,232,156 + +1.0
3,232,172 + -0.2
3,232,349 + PFLU_RS14420 0.38 +1.4
3,232,357 - PFLU_RS14420 0.40 -0.8
3,232,357 - PFLU_RS14420 0.40 -0.2
3,232,487 + PFLU_RS14420 0.61 +1.6
3,232,487 + PFLU_RS14420 0.61 -1.0
3,232,488 - PFLU_RS14420 0.61 +1.5
3,232,488 - PFLU_RS14420 0.61 +1.5
3,232,633 + PFLU_RS14420 0.85 +0.5
3,232,696 + +1.6
3,232,707 - +0.9
3,232,765 - +0.8
3,232,955 + PFLU_RS14425 0.20 -0.2
3,232,955 + PFLU_RS14425 0.20 +2.4
3,232,956 - PFLU_RS14425 0.20 -0.6
3,232,956 - PFLU_RS14425 0.20 -0.6
3,232,956 - PFLU_RS14425 0.20 -0.8
3,232,956 - PFLU_RS14425 0.20 -0.6
3,232,956 - PFLU_RS14425 0.20 -0.6
3,232,956 - PFLU_RS14425 0.20 +0.1
3,233,044 + PFLU_RS14425 0.31 -0.6
3,233,044 + PFLU_RS14425 0.31 -0.6
3,233,045 - PFLU_RS14425 0.31 -0.9
3,233,045 - PFLU_RS14425 0.31 +0.3
3,233,045 - PFLU_RS14425 0.31 +1.4
3,233,045 - PFLU_RS14425 0.31 +0.1
3,233,045 - PFLU_RS14425 0.31 +1.4
3,233,237 + PFLU_RS14425 0.55 -1.2
3,233,237 + PFLU_RS14425 0.55 -0.1
3,233,303 + PFLU_RS14425 0.64 -0.4
3,233,303 + PFLU_RS14425 0.64 +0.8
3,233,303 + PFLU_RS14425 0.64 +0.1
3,233,344 - PFLU_RS14425 0.69 -0.6
3,233,344 - PFLU_RS14425 0.69 -0.8
3,233,409 + PFLU_RS14425 0.77 -0.9
3,233,409 + PFLU_RS14425 0.77 -1.1
3,233,409 + PFLU_RS14425 0.77 -0.9
3,233,409 + PFLU_RS14425 0.77 +1.7
3,233,409 + PFLU_RS14425 0.77 +1.3
3,233,410 - PFLU_RS14425 0.77 -0.7
3,233,410 - PFLU_RS14425 0.77 -0.0
3,233,410 - PFLU_RS14425 0.77 +0.2
3,233,410 - PFLU_RS14425 0.77 +0.6
3,233,410 - PFLU_RS14425 0.77 +1.0
3,233,410 - PFLU_RS14425 0.77 +0.4
3,233,410 - PFLU_RS14425 0.77 -0.9
3,233,410 - PFLU_RS14425 0.77 -0.4
3,233,410 - PFLU_RS14425 0.77 -0.4
3,233,416 + PFLU_RS14425 0.78 -0.4
3,233,417 - PFLU_RS14425 0.78 +2.4
3,233,635 + -1.1

Or see this region's nucleotide sequence