Experiment: 1-ethyl-3-methylimidazolium acetate 20 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0519 and Psest_0520 are separated by 202 nucleotides Psest_0520 and Psest_0521 are separated by 86 nucleotides Psest_0521 and Psest_0522 are separated by 62 nucleotides Psest_0522 and Psest_0523 are separated by 93 nucleotides
Psest_0519: Psest_0519 - lipoate synthase, at 572,004 to 572,981
_0519
Psest_0520: Psest_0520 - hypothetical protein, at 573,184 to 573,528
_0520
Psest_0521: Psest_0521 - lytic murein transglycosylase, at 573,615 to 574,913
_0521
Psest_0522: Psest_0522 - Uncharacterized protein conserved in bacteria, at 574,976 to 575,134
_0522
Psest_0523: Psest_0523 - DNA polymerase III, delta subunit, at 575,228 to 576,256
_0523
Position (kb) 573
574
575 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 573.125 kb on - strand at 573.125 kb on - strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.136 kb on + strand at 573.137 kb on - strand at 573.138 kb on + strand at 573.153 kb on - strand at 573.153 kb on - strand at 573.200 kb on + strand at 573.382 kb on + strand, within Psest_0520 at 573.500 kb on - strand at 573.500 kb on - strand at 573.505 kb on + strand at 573.505 kb on + strand at 573.506 kb on - strand at 573.552 kb on + strand at 573.552 kb on + strand at 573.553 kb on - strand at 573.886 kb on + strand, within Psest_0521 at 573.886 kb on + strand, within Psest_0521 at 573.904 kb on + strand, within Psest_0521 at 573.904 kb on + strand, within Psest_0521 at 573.926 kb on + strand, within Psest_0521 at 573.927 kb on - strand, within Psest_0521 at 574.144 kb on + strand, within Psest_0521 at 574.144 kb on + strand, within Psest_0521 at 574.145 kb on - strand, within Psest_0521 at 574.145 kb on - strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.231 kb on + strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.232 kb on - strand, within Psest_0521 at 574.571 kb on - strand, within Psest_0521 at 574.677 kb on - strand, within Psest_0521 at 574.677 kb on - strand, within Psest_0521 at 574.772 kb on + strand, within Psest_0521 at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.909 kb on + strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.910 kb on - strand at 574.951 kb on - strand at 574.951 kb on - strand at 574.951 kb on - strand at 575.177 kb on + strand at 575.178 kb on - strand at 575.178 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 1-ethyl-3-methylimidazolium acetate 20 mM remove 573,125 - +1.8 573,125 - +0.2 573,136 + +0.2 573,136 + -0.0 573,136 + -0.5 573,136 + -2.0 573,136 + +0.2 573,136 + -1.8 573,137 - -0.7 573,138 + +0.0 573,153 - -0.3 573,153 - -0.7 573,200 + -1.3 573,382 + Psest_0520 0.57 -1.2 573,500 - -0.6 573,500 - -0.3 573,505 + +1.2 573,505 + -0.9 573,506 - +1.0 573,552 + +1.6 573,552 + +0.3 573,553 - -1.9 573,886 + Psest_0521 0.21 -0.6 573,886 + Psest_0521 0.21 -1.2 573,904 + Psest_0521 0.22 -0.9 573,904 + Psest_0521 0.22 -1.3 573,926 + Psest_0521 0.24 -0.5 573,927 - Psest_0521 0.24 -1.3 574,144 + Psest_0521 0.41 -1.9 574,144 + Psest_0521 0.41 -0.6 574,145 - Psest_0521 0.41 +0.2 574,145 - Psest_0521 0.41 -1.5 574,231 + Psest_0521 0.47 -1.5 574,231 + Psest_0521 0.47 +0.9 574,231 + Psest_0521 0.47 -0.6 574,231 + Psest_0521 0.47 +1.2 574,231 + Psest_0521 0.47 +0.2 574,231 + Psest_0521 0.47 +0.1 574,231 + Psest_0521 0.47 -2.5 574,231 + Psest_0521 0.47 -3.1 574,231 + Psest_0521 0.47 -0.8 574,231 + Psest_0521 0.47 -1.2 574,231 + Psest_0521 0.47 -0.3 574,231 + Psest_0521 0.47 +0.4 574,231 + Psest_0521 0.47 +0.9 574,231 + Psest_0521 0.47 -0.8 574,231 + Psest_0521 0.47 -0.8 574,232 - Psest_0521 0.47 -1.1 574,232 - Psest_0521 0.47 +0.2 574,232 - Psest_0521 0.47 -3.6 574,232 - Psest_0521 0.47 -2.0 574,232 - Psest_0521 0.47 -0.4 574,232 - Psest_0521 0.47 -0.2 574,232 - Psest_0521 0.47 -1.7 574,232 - Psest_0521 0.47 +0.7 574,232 - Psest_0521 0.47 -0.9 574,232 - Psest_0521 0.47 -0.1 574,232 - Psest_0521 0.47 -1.9 574,232 - Psest_0521 0.47 -1.1 574,232 - Psest_0521 0.47 -0.1 574,232 - Psest_0521 0.47 -1.0 574,571 - Psest_0521 0.74 +0.4 574,677 - Psest_0521 0.82 +0.1 574,677 - Psest_0521 0.82 -2.0 574,772 + Psest_0521 0.89 -1.4 574,909 + +2.3 574,909 + +0.7 574,909 + +0.2 574,909 + -2.8 574,909 + -1.0 574,909 + +0.1 574,909 + +0.4 574,909 + -0.5 574,909 + +1.1 574,909 + -0.8 574,910 - -1.3 574,910 - -2.0 574,910 - +0.3 574,910 - +0.4 574,910 - -0.9 574,910 - -0.5 574,951 - +0.7 574,951 - +0.3 574,951 - +0.2 575,177 + -0.5 575,178 - -0.3 575,178 - +0.6
Or see this region's nucleotide sequence