Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_121

Experiment: Nitrate reduction, replicate A

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_120 and Pf6N2E2_121 are separated by 17 nucleotidesPf6N2E2_121 and Pf6N2E2_122 are separated by 20 nucleotidesPf6N2E2_122 and Pf6N2E2_123 are separated by 0 nucleotides Pf6N2E2_120 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 167,200 to 168,792 _120 Pf6N2E2_121 - probable polysaccharide biosynthesis protein, at 168,810 to 169,832 _121 Pf6N2E2_122 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 169,853 to 170,497 _122 Pf6N2E2_123 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 170,498 to 171,244 _123 Position (kb) 168 169 170Strain fitness (log2 ratio) -2 -1 0 1 2 3at 167.906 kb on + strand, within Pf6N2E2_120at 167.906 kb on + strand, within Pf6N2E2_120at 167.906 kb on + strand, within Pf6N2E2_120at 167.907 kb on - strand, within Pf6N2E2_120at 167.907 kb on - strand, within Pf6N2E2_120at 167.954 kb on + strand, within Pf6N2E2_120at 167.955 kb on - strand, within Pf6N2E2_120at 167.994 kb on - strand, within Pf6N2E2_120at 168.125 kb on + strand, within Pf6N2E2_120at 168.126 kb on - strand, within Pf6N2E2_120at 168.146 kb on + strand, within Pf6N2E2_120at 168.146 kb on + strand, within Pf6N2E2_120at 168.146 kb on + strand, within Pf6N2E2_120at 168.146 kb on + strand, within Pf6N2E2_120at 168.147 kb on - strand, within Pf6N2E2_120at 168.197 kb on + strand, within Pf6N2E2_120at 168.197 kb on + strand, within Pf6N2E2_120at 168.254 kb on + strand, within Pf6N2E2_120at 168.254 kb on + strand, within Pf6N2E2_120at 168.327 kb on + strand, within Pf6N2E2_120at 168.395 kb on + strand, within Pf6N2E2_120at 168.396 kb on - strand, within Pf6N2E2_120at 168.419 kb on + strand, within Pf6N2E2_120at 168.420 kb on - strand, within Pf6N2E2_120at 168.473 kb on + strand, within Pf6N2E2_120at 168.474 kb on - strand, within Pf6N2E2_120at 168.474 kb on - strand, within Pf6N2E2_120at 168.720 kb on + strandat 168.720 kb on + strandat 168.721 kb on - strandat 168.721 kb on - strandat 168.773 kb on + strandat 168.876 kb on + strandat 168.876 kb on + strandat 168.934 kb on + strand, within Pf6N2E2_121at 168.934 kb on + strand, within Pf6N2E2_121at 168.934 kb on + strand, within Pf6N2E2_121at 168.935 kb on - strand, within Pf6N2E2_121at 168.958 kb on + strand, within Pf6N2E2_121at 169.003 kb on + strand, within Pf6N2E2_121at 169.003 kb on + strand, within Pf6N2E2_121at 169.129 kb on + strand, within Pf6N2E2_121at 169.159 kb on - strand, within Pf6N2E2_121at 169.159 kb on - strand, within Pf6N2E2_121at 169.159 kb on - strand, within Pf6N2E2_121at 169.159 kb on - strand, within Pf6N2E2_121at 169.162 kb on + strand, within Pf6N2E2_121at 169.162 kb on + strand, within Pf6N2E2_121at 169.162 kb on + strand, within Pf6N2E2_121at 169.162 kb on + strand, within Pf6N2E2_121at 169.162 kb on + strand, within Pf6N2E2_121at 169.163 kb on - strand, within Pf6N2E2_121at 169.163 kb on - strand, within Pf6N2E2_121at 169.163 kb on - strand, within Pf6N2E2_121at 169.163 kb on - strand, within Pf6N2E2_121at 169.163 kb on - strand, within Pf6N2E2_121at 169.163 kb on - strand, within Pf6N2E2_121at 169.163 kb on - strand, within Pf6N2E2_121at 169.310 kb on - strand, within Pf6N2E2_121at 169.627 kb on + strand, within Pf6N2E2_121at 169.854 kb on + strandat 169.854 kb on + strandat 169.854 kb on + strandat 169.854 kb on + strandat 169.855 kb on - strandat 169.855 kb on - strandat 169.866 kb on + strandat 169.866 kb on + strandat 169.884 kb on + strandat 169.885 kb on - strandat 169.887 kb on + strandat 169.888 kb on - strandat 170.046 kb on + strand, within Pf6N2E2_122at 170.079 kb on + strand, within Pf6N2E2_122at 170.499 kb on + strandat 170.500 kb on - strandat 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.583 kb on + strand, within Pf6N2E2_123at 170.584 kb on - strand, within Pf6N2E2_123at 170.584 kb on - strand, within Pf6N2E2_123at 170.584 kb on - strand, within Pf6N2E2_123at 170.664 kb on + strand, within Pf6N2E2_123at 170.664 kb on + strand, within Pf6N2E2_123at 170.664 kb on + strand, within Pf6N2E2_123at 170.697 kb on + strand, within Pf6N2E2_123at 170.697 kb on + strand, within Pf6N2E2_123at 170.703 kb on + strand, within Pf6N2E2_123at 170.756 kb on + strand, within Pf6N2E2_123at 170.756 kb on + strand, within Pf6N2E2_123at 170.757 kb on - strand, within Pf6N2E2_123at 170.757 kb on - strand, within Pf6N2E2_123at 170.774 kb on + strand, within Pf6N2E2_123

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction, replicate A
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167,906 + Pf6N2E2_120 0.44 +0.1
167,906 + Pf6N2E2_120 0.44 -0.7
167,906 + Pf6N2E2_120 0.44 -0.3
167,907 - Pf6N2E2_120 0.44 +0.8
167,907 - Pf6N2E2_120 0.44 -0.6
167,954 + Pf6N2E2_120 0.47 +0.4
167,955 - Pf6N2E2_120 0.47 -0.4
167,994 - Pf6N2E2_120 0.50 +0.7
168,125 + Pf6N2E2_120 0.58 +0.4
168,126 - Pf6N2E2_120 0.58 +0.7
168,146 + Pf6N2E2_120 0.59 -0.2
168,146 + Pf6N2E2_120 0.59 +1.2
168,146 + Pf6N2E2_120 0.59 -1.4
168,146 + Pf6N2E2_120 0.59 +1.5
168,147 - Pf6N2E2_120 0.59 -0.0
168,197 + Pf6N2E2_120 0.63 -0.8
168,197 + Pf6N2E2_120 0.63 +0.3
168,254 + Pf6N2E2_120 0.66 +0.2
168,254 + Pf6N2E2_120 0.66 +0.2
168,327 + Pf6N2E2_120 0.71 -0.5
168,395 + Pf6N2E2_120 0.75 +0.5
168,396 - Pf6N2E2_120 0.75 +0.2
168,419 + Pf6N2E2_120 0.77 +0.2
168,420 - Pf6N2E2_120 0.77 -1.2
168,473 + Pf6N2E2_120 0.80 -0.2
168,474 - Pf6N2E2_120 0.80 +0.4
168,474 - Pf6N2E2_120 0.80 +0.2
168,720 + -0.9
168,720 + -0.1
168,721 - +0.4
168,721 - +0.2
168,773 + -0.6
168,876 + -1.1
168,876 + +2.0
168,934 + Pf6N2E2_121 0.12 +1.8
168,934 + Pf6N2E2_121 0.12 +0.4
168,934 + Pf6N2E2_121 0.12 -0.2
168,935 - Pf6N2E2_121 0.12 +1.8
168,958 + Pf6N2E2_121 0.14 +0.7
169,003 + Pf6N2E2_121 0.19 -1.4
169,003 + Pf6N2E2_121 0.19 -1.2
169,129 + Pf6N2E2_121 0.31 -0.3
169,159 - Pf6N2E2_121 0.34 +1.5
169,159 - Pf6N2E2_121 0.34 +0.7
169,159 - Pf6N2E2_121 0.34 +1.1
169,159 - Pf6N2E2_121 0.34 +1.5
169,162 + Pf6N2E2_121 0.34 +0.4
169,162 + Pf6N2E2_121 0.34 -0.2
169,162 + Pf6N2E2_121 0.34 +0.0
169,162 + Pf6N2E2_121 0.34 -0.2
169,162 + Pf6N2E2_121 0.34 -0.0
169,163 - Pf6N2E2_121 0.35 +1.6
169,163 - Pf6N2E2_121 0.35 +2.8
169,163 - Pf6N2E2_121 0.35 -0.6
169,163 - Pf6N2E2_121 0.35 +1.4
169,163 - Pf6N2E2_121 0.35 +0.7
169,163 - Pf6N2E2_121 0.35 +1.2
169,163 - Pf6N2E2_121 0.35 +0.9
169,310 - Pf6N2E2_121 0.49 +0.8
169,627 + Pf6N2E2_121 0.80 -1.4
169,854 + -0.2
169,854 + -0.6
169,854 + +1.0
169,854 + +1.4
169,855 - +2.2
169,855 - +1.0
169,866 + +0.8
169,866 + -0.7
169,884 + -0.2
169,885 - +1.8
169,887 + +0.4
169,888 - +2.2
170,046 + Pf6N2E2_122 0.30 -1.8
170,079 + Pf6N2E2_122 0.35 +0.0
170,499 + -1.0
170,500 - -0.4
170,583 + Pf6N2E2_123 0.11 +1.9
170,583 + Pf6N2E2_123 0.11 +0.6
170,583 + Pf6N2E2_123 0.11 -0.5
170,583 + Pf6N2E2_123 0.11 -0.6
170,583 + Pf6N2E2_123 0.11 +0.0
170,584 - Pf6N2E2_123 0.12 +1.4
170,584 - Pf6N2E2_123 0.12 +1.8
170,584 - Pf6N2E2_123 0.12 +2.1
170,664 + Pf6N2E2_123 0.22 +0.4
170,664 + Pf6N2E2_123 0.22 -1.6
170,664 + Pf6N2E2_123 0.22 +0.4
170,697 + Pf6N2E2_123 0.27 +1.0
170,697 + Pf6N2E2_123 0.27 -0.5
170,703 + Pf6N2E2_123 0.27 +0.8
170,756 + Pf6N2E2_123 0.35 -2.6
170,756 + Pf6N2E2_123 0.35 -0.2
170,757 - Pf6N2E2_123 0.35 +2.3
170,757 - Pf6N2E2_123 0.35 +0.8
170,774 + Pf6N2E2_123 0.37 -0.9

Or see this region's nucleotide sequence