Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_450

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_448 and Pf6N2E2_449 are separated by 87 nucleotidesPf6N2E2_449 and Pf6N2E2_450 are separated by 194 nucleotidesPf6N2E2_450 and Pf6N2E2_451 are separated by 110 nucleotidesPf6N2E2_451 and Pf6N2E2_452 are separated by 211 nucleotides Pf6N2E2_448 - Alkyl hydroperoxide reductase protein F (EC 1.6.4.-), at 510,388 to 511,950 _448 Pf6N2E2_449 - Lactoylglutathione lyase (EC 4.4.1.5), at 512,038 to 512,559 _449 Pf6N2E2_450 - FIG00953950: hypothetical protein, at 512,754 to 513,281 _450 Pf6N2E2_451 - transcriptional regulator MvaT, P16 subunit, putative, at 513,392 to 513,757 _451 Pf6N2E2_452 - Rtn protein, at 513,969 to 515,513 _452 Position (kb) 512 513 514Strain fitness (log2 ratio) -2 -1 0 1 2at 511.861 kb on + strandat 511.861 kb on + strandat 511.862 kb on - strandat 511.948 kb on + strandat 512.039 kb on + strandat 512.040 kb on - strandat 512.072 kb on + strandat 512.073 kb on - strandat 512.237 kb on + strand, within Pf6N2E2_449at 512.370 kb on - strand, within Pf6N2E2_449at 512.370 kb on - strand, within Pf6N2E2_449at 512.370 kb on - strand, within Pf6N2E2_449at 512.435 kb on + strand, within Pf6N2E2_449at 512.573 kb on - strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.574 kb on + strandat 512.575 kb on - strandat 512.575 kb on - strandat 512.575 kb on - strandat 512.575 kb on - strandat 512.575 kb on - strandat 512.658 kb on + strandat 512.659 kb on - strandat 512.659 kb on - strandat 512.659 kb on - strandat 512.659 kb on - strandat 513.125 kb on + strand, within Pf6N2E2_450at 513.125 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.151 kb on + strand, within Pf6N2E2_450at 513.152 kb on - strand, within Pf6N2E2_450at 513.152 kb on - strand, within Pf6N2E2_450at 513.152 kb on - strandat 513.152 kb on - strand, within Pf6N2E2_450at 513.152 kb on - strand, within Pf6N2E2_450at 513.152 kb on - strand, within Pf6N2E2_450at 513.152 kb on - strand, within Pf6N2E2_450at 513.178 kb on + strand, within Pf6N2E2_450at 513.179 kb on - strand, within Pf6N2E2_450at 513.179 kb on - strand, within Pf6N2E2_450at 513.195 kb on + strand, within Pf6N2E2_450at 513.195 kb on + strand, within Pf6N2E2_450at 513.195 kb on + strand, within Pf6N2E2_450at 513.195 kb on + strand, within Pf6N2E2_450at 513.195 kb on + strand, within Pf6N2E2_450at 513.195 kb on + strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.196 kb on - strand, within Pf6N2E2_450at 513.208 kb on - strand, within Pf6N2E2_450at 513.208 kb on - strand, within Pf6N2E2_450at 513.250 kb on + strandat 513.251 kb on - strandat 513.278 kb on - strandat 513.296 kb on + strandat 513.430 kb on - strand, within Pf6N2E2_451at 513.430 kb on - strand, within Pf6N2E2_451at 513.516 kb on - strand, within Pf6N2E2_451at 513.779 kb on - strandat 513.779 kb on - strandat 513.811 kb on - strandat 513.811 kb on - strandat 513.846 kb on - strandat 513.859 kb on - strandat 513.873 kb on - strandat 513.873 kb on - strandat 513.914 kb on - strandat 513.932 kb on + strandat 513.955 kb on + strandat 513.980 kb on + strandat 514.032 kb on + strandat 514.032 kb on + strandat 514.032 kb on + strandat 514.032 kb on + strandat 514.033 kb on - strandat 514.036 kb on + strandat 514.037 kb on - strandat 514.045 kb on - strandat 514.240 kb on + strand, within Pf6N2E2_452at 514.240 kb on + strand, within Pf6N2E2_452at 514.241 kb on - strand, within Pf6N2E2_452

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Per-strain Table

Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C
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511,861 + +0.2
511,861 + -2.2
511,862 - -1.0
511,948 + -1.2
512,039 + -0.3
512,040 - -1.0
512,072 + -0.5
512,073 - -1.0
512,237 + Pf6N2E2_449 0.38 +0.3
512,370 - Pf6N2E2_449 0.64 -0.1
512,370 - Pf6N2E2_449 0.64 +0.4
512,370 - Pf6N2E2_449 0.64 -0.2
512,435 + Pf6N2E2_449 0.76 +2.0
512,573 - -0.7
512,574 + +0.1
512,574 + +0.6
512,574 + -1.3
512,574 + +1.4
512,574 + +2.4
512,574 + -0.6
512,574 + -0.8
512,574 + -0.4
512,574 + +0.1
512,575 - +0.4
512,575 - +0.7
512,575 - +0.0
512,575 - -1.6
512,575 - -0.0
512,658 + +0.8
512,659 - +0.4
512,659 - +1.4
512,659 - -1.0
512,659 - -0.7
513,125 + Pf6N2E2_450 0.70 +0.1
513,125 + Pf6N2E2_450 0.70 -0.3
513,151 + Pf6N2E2_450 0.75 -1.4
513,151 + Pf6N2E2_450 0.75 +0.3
513,151 + Pf6N2E2_450 0.75 +0.3
513,151 + Pf6N2E2_450 0.75 -1.2
513,151 + Pf6N2E2_450 0.75 -0.3
513,151 + Pf6N2E2_450 0.75 -0.2
513,151 + Pf6N2E2_450 0.75 +0.5
513,151 + Pf6N2E2_450 0.75 -0.4
513,152 - Pf6N2E2_450 0.75 +0.4
513,152 - Pf6N2E2_450 0.75 +0.4
513,152 - +1.4
513,152 - Pf6N2E2_450 0.75 -0.4
513,152 - Pf6N2E2_450 0.75 +0.2
513,152 - Pf6N2E2_450 0.75 -0.6
513,152 - Pf6N2E2_450 0.75 +0.8
513,178 + Pf6N2E2_450 0.80 +0.2
513,179 - Pf6N2E2_450 0.80 +0.1
513,179 - Pf6N2E2_450 0.80 -0.2
513,195 + Pf6N2E2_450 0.84 +0.3
513,195 + Pf6N2E2_450 0.84 +0.6
513,195 + Pf6N2E2_450 0.84 -0.4
513,195 + Pf6N2E2_450 0.84 -0.1
513,195 + Pf6N2E2_450 0.84 +0.4
513,195 + Pf6N2E2_450 0.84 +0.4
513,196 - Pf6N2E2_450 0.84 +0.0
513,196 - Pf6N2E2_450 0.84 -0.7
513,196 - Pf6N2E2_450 0.84 +1.4
513,196 - Pf6N2E2_450 0.84 -0.2
513,196 - Pf6N2E2_450 0.84 -0.4
513,196 - Pf6N2E2_450 0.84 -0.1
513,196 - Pf6N2E2_450 0.84 +0.1
513,196 - Pf6N2E2_450 0.84 -0.3
513,208 - Pf6N2E2_450 0.86 -1.9
513,208 - Pf6N2E2_450 0.86 -0.2
513,250 + -1.3
513,251 - -0.4
513,278 - +0.4
513,296 + -0.2
513,430 - Pf6N2E2_451 0.10 -0.8
513,430 - Pf6N2E2_451 0.10 -1.4
513,516 - Pf6N2E2_451 0.34 +0.2
513,779 - +0.4
513,779 - -0.2
513,811 - +1.4
513,811 - -2.1
513,846 - -0.4
513,859 - -0.2
513,873 - -0.7
513,873 - -0.2
513,914 - -0.7
513,932 + -0.3
513,955 + -0.6
513,980 + -0.3
514,032 + +1.8
514,032 + -0.3
514,032 + +0.8
514,032 + -0.2
514,033 - +0.2
514,036 + -0.5
514,037 - -0.3
514,045 - +0.3
514,240 + Pf6N2E2_452 0.18 +0.3
514,240 + Pf6N2E2_452 0.18 -0.6
514,241 - Pf6N2E2_452 0.18 +0.2

Or see this region's nucleotide sequence