Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_726 and Pf6N2E2_727 are separated by 308 nucleotides Pf6N2E2_727 and Pf6N2E2_728 overlap by 23 nucleotides
Pf6N2E2_726 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 829,387 to 831,186
_726
Pf6N2E2_727 - Nitrate/nitrite transporter, at 831,495 to 832,796
_727
Pf6N2E2_728 - Nitrate/nitrite transporter, at 832,774 to 834,171
_728
Position (kb) 831
832
833 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 830.667 kb on - strand, within Pf6N2E2_726 at 830.882 kb on + strand, within Pf6N2E2_726 at 831.020 kb on + strand at 831.020 kb on + strand at 831.021 kb on - strand at 831.021 kb on - strand at 831.021 kb on - strand at 831.251 kb on - strand at 831.251 kb on - strand at 831.272 kb on + strand at 831.272 kb on + strand at 831.272 kb on + strand at 831.273 kb on - strand at 831.334 kb on - strand at 831.354 kb on - strand at 831.383 kb on + strand at 831.384 kb on - strand at 831.398 kb on + strand at 831.459 kb on + strand at 831.459 kb on + strand at 831.459 kb on + strand at 831.459 kb on + strand at 831.460 kb on - strand at 831.523 kb on + strand at 831.523 kb on + strand at 831.536 kb on + strand at 831.536 kb on + strand at 831.759 kb on + strand, within Pf6N2E2_727 at 831.760 kb on - strand, within Pf6N2E2_727 at 831.852 kb on + strand, within Pf6N2E2_727 at 831.852 kb on + strand, within Pf6N2E2_727 at 831.852 kb on + strand, within Pf6N2E2_727 at 832.134 kb on + strand, within Pf6N2E2_727 at 832.135 kb on - strand, within Pf6N2E2_727 at 832.135 kb on - strand, within Pf6N2E2_727 at 832.137 kb on + strand, within Pf6N2E2_727 at 832.159 kb on - strand, within Pf6N2E2_727 at 832.159 kb on - strand, within Pf6N2E2_727 at 832.188 kb on + strand, within Pf6N2E2_727 at 832.188 kb on + strand, within Pf6N2E2_727 at 832.189 kb on - strand, within Pf6N2E2_727 at 832.189 kb on - strand, within Pf6N2E2_727 at 832.189 kb on - strand, within Pf6N2E2_727 at 832.189 kb on - strand, within Pf6N2E2_727 at 832.191 kb on + strand, within Pf6N2E2_727 at 832.192 kb on - strand, within Pf6N2E2_727 at 832.192 kb on - strand, within Pf6N2E2_727 at 832.192 kb on - strand, within Pf6N2E2_727 at 832.192 kb on - strand, within Pf6N2E2_727 at 832.192 kb on - strand, within Pf6N2E2_727 at 832.192 kb on - strand, within Pf6N2E2_727 at 832.503 kb on + strand, within Pf6N2E2_727 at 832.503 kb on + strand, within Pf6N2E2_727 at 832.504 kb on - strand, within Pf6N2E2_727 at 832.504 kb on - strand, within Pf6N2E2_727 at 832.650 kb on + strand, within Pf6N2E2_727 at 832.650 kb on + strand, within Pf6N2E2_727 at 832.651 kb on - strand, within Pf6N2E2_727 at 832.651 kb on - strand, within Pf6N2E2_727 at 832.651 kb on - strand, within Pf6N2E2_727 at 832.683 kb on + strand at 832.683 kb on + strand at 832.683 kb on + strand at 832.683 kb on + strand at 832.683 kb on + strand at 832.684 kb on - strand at 832.684 kb on - strand at 832.684 kb on - strand at 833.042 kb on - strand, within Pf6N2E2_728 at 833.239 kb on + strand, within Pf6N2E2_728 at 833.375 kb on + strand, within Pf6N2E2_728
Per-strain Table
Position Strand Gene LocusTag Fraction Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate B remove 830,667 - Pf6N2E2_726 0.71 -2.4 830,882 + Pf6N2E2_726 0.83 -2.3 831,020 + -4.0 831,020 + -3.3 831,021 - -0.8 831,021 - -1.1 831,021 - -3.2 831,251 - -0.5 831,251 - -0.6 831,272 + -2.4 831,272 + -0.6 831,272 + -1.3 831,273 - -2.0 831,334 - -4.5 831,354 - -1.9 831,383 + -2.6 831,384 - -3.0 831,398 + -0.2 831,459 + +0.6 831,459 + -1.4 831,459 + +0.4 831,459 + -0.8 831,460 - -1.6 831,523 + -0.9 831,523 + -1.7 831,536 + +0.9 831,536 + -1.9 831,759 + Pf6N2E2_727 0.20 +1.5 831,760 - Pf6N2E2_727 0.20 +1.5 831,852 + Pf6N2E2_727 0.27 -3.3 831,852 + Pf6N2E2_727 0.27 -1.9 831,852 + Pf6N2E2_727 0.27 -1.6 832,134 + Pf6N2E2_727 0.49 +0.5 832,135 - Pf6N2E2_727 0.49 -3.9 832,135 - Pf6N2E2_727 0.49 -2.7 832,137 + Pf6N2E2_727 0.49 -0.8 832,159 - Pf6N2E2_727 0.51 -1.0 832,159 - Pf6N2E2_727 0.51 -1.3 832,188 + Pf6N2E2_727 0.53 -1.8 832,188 + Pf6N2E2_727 0.53 -2.3 832,189 - Pf6N2E2_727 0.53 -3.1 832,189 - Pf6N2E2_727 0.53 -2.1 832,189 - Pf6N2E2_727 0.53 -1.1 832,189 - Pf6N2E2_727 0.53 -2.9 832,191 + Pf6N2E2_727 0.53 -1.5 832,192 - Pf6N2E2_727 0.54 -0.1 832,192 - Pf6N2E2_727 0.54 -3.6 832,192 - Pf6N2E2_727 0.54 +0.5 832,192 - Pf6N2E2_727 0.54 +1.5 832,192 - Pf6N2E2_727 0.54 -1.8 832,192 - Pf6N2E2_727 0.54 -1.6 832,503 + Pf6N2E2_727 0.77 +1.5 832,503 + Pf6N2E2_727 0.77 -0.5 832,504 - Pf6N2E2_727 0.77 -2.4 832,504 - Pf6N2E2_727 0.77 -0.1 832,650 + Pf6N2E2_727 0.89 +1.5 832,650 + Pf6N2E2_727 0.89 +0.7 832,651 - Pf6N2E2_727 0.89 -1.5 832,651 - Pf6N2E2_727 0.89 +0.5 832,651 - Pf6N2E2_727 0.89 -2.1 832,683 + +1.3 832,683 + +0.1 832,683 + +0.5 832,683 + +0.9 832,683 + +0.1 832,684 - -1.3 832,684 - +1.5 832,684 - -1.9 833,042 - Pf6N2E2_728 0.19 -3.4 833,239 + Pf6N2E2_728 0.33 +1.5 833,375 + Pf6N2E2_728 0.43 -3.6
Or see this region's nucleotide sequence