Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1961

Experiment: Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	Nitrate reduction in presence of wild-type Pseudomonas N1-B4; replicate A
2,325,232	-		Pf6N2E2_1960	0.40	+0.3
2,325,232	-		Pf6N2E2_1960	0.40	-0.7
2,325,330	+		Pf6N2E2_1960	0.48	-1.0
2,325,331	-		Pf6N2E2_1960	0.49	+1.6
2,325,331	-		Pf6N2E2_1960	0.49	+0.8
2,325,355	+		Pf6N2E2_1960	0.51	-0.3
2,325,387	-		Pf6N2E2_1960	0.54	-0.9
2,325,502	+		Pf6N2E2_1960	0.64	+1.3
2,325,502	+		Pf6N2E2_1960	0.64	-0.7
2,325,503	-		Pf6N2E2_1960	0.64	+0.0
2,325,645	+		Pf6N2E2_1960	0.77	-0.7
2,325,645	+		Pf6N2E2_1960	0.77	-0.1
2,325,645	+		Pf6N2E2_1960	0.77	+1.6
2,325,646	-		Pf6N2E2_1960	0.77	-0.0
2,325,646	-		Pf6N2E2_1960	0.77	-1.7
2,325,646	-		Pf6N2E2_1960	0.77	+0.6
2,325,900	-				+0.1
2,326,021	+				+1.3
2,326,021	+				+0.3
2,326,021	+				+1.2
2,326,022	-				+0.2
2,326,022	-				+1.8
2,326,022	-				-1.4
2,326,299	+		Pf6N2E2_1961	0.41	-1.2
2,326,306	-		Pf6N2E2_1961	0.42	+0.6
2,326,345	-		Pf6N2E2_1961	0.47	-0.6
2,326,345	-		Pf6N2E2_1961	0.47	+0.5
2,326,345	-		Pf6N2E2_1961	0.47	-2.3
2,326,401	+		Pf6N2E2_1961	0.54	-0.8
2,326,401	+		Pf6N2E2_1961	0.54	-1.4
2,326,401	+		Pf6N2E2_1961	0.54	-1.4
2,326,401	+		Pf6N2E2_1961	0.54	+1.6
2,326,401	+		Pf6N2E2_1961	0.54	-1.2
2,326,401	+		Pf6N2E2_1961	0.54	-0.4
2,326,405	+		Pf6N2E2_1961	0.54	+0.2
2,326,406	-		Pf6N2E2_1961	0.54	-0.0
2,326,406	-		Pf6N2E2_1961	0.54	-1.1
2,326,581	+		Pf6N2E2_1961	0.75	-0.7
2,326,582	-		Pf6N2E2_1961	0.75	-0.6
2,326,608	+		Pf6N2E2_1961	0.79	+0.6
2,326,609	-		Pf6N2E2_1961	0.79	-1.5
2,326,622	-		Pf6N2E2_1961	0.80	-0.5
2,326,748	+				-1.4
2,326,774	+				-0.4
2,326,774	+				+2.6
2,326,927	+		Pf6N2E2_1962	0.15	+0.1
2,327,337	-		Pf6N2E2_1962	0.64	+1.6
2,327,708	+				+2.0
2,327,727	+				+0.8
2,327,732	-				-0.4
2,327,773	-				-0.1
2,327,784	+				-0.2
2,327,785	-				-1.2