Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS22795 and RALBFv3_RS22800 overlap by 56 nucleotides RALBFv3_RS22800 and RALBFv3_RS22805 are separated by 150 nucleotides RALBFv3_RS22805 and RALBFv3_RS22810 overlap by 4 nucleotides RALBFv3_RS22810 and RALBFv3_RS22815 are separated by 50 nucleotides
RALBFv3_RS22795: RALBFv3_RS22795 - type II toxin-antitoxin system antitoxin, RelB/DinJ family, at 1,886,139 to 1,886,450
_RS22795
RALBFv3_RS22800: RALBFv3_RS22800 - type II toxin-antitoxin system YafQ family toxin, at 1,886,395 to 1,886,703
_RS22800
RALBFv3_RS22805: RALBFv3_RS22805 - transcriptional regulator, at 1,886,854 to 1,887,165
_RS22805
RALBFv3_RS22810: RALBFv3_RS22810 - hypothetical protein, at 1,887,162 to 1,887,491
_RS22810
RALBFv3_RS22815: RALBFv3_RS22815 - propionate catabolism operon regulatory protein PrpR, at 1,887,542 to 1,889,491
_RS22815
Position (kb)
1886
1887
1888 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1886.019 kb on + strand at 1886.019 kb on + strand at 1886.019 kb on + strand at 1886.020 kb on - strand at 1886.020 kb on - strand at 1886.020 kb on - strand at 1886.020 kb on - strand at 1886.020 kb on - strand at 1886.104 kb on + strand at 1886.104 kb on + strand at 1886.104 kb on + strand at 1886.104 kb on + strand at 1886.104 kb on + strand at 1886.104 kb on + strand at 1886.104 kb on + strand at 1886.122 kb on + strand at 1886.122 kb on + strand at 1886.122 kb on + strand at 1886.123 kb on - strand at 1886.123 kb on - strand at 1886.126 kb on + strand at 1886.126 kb on + strand at 1886.126 kb on + strand at 1886.126 kb on + strand at 1886.126 kb on + strand at 1886.392 kb on + strand, within RALBFv3_RS22795 at 1886.392 kb on + strand, within RALBFv3_RS22795 at 1886.392 kb on + strand, within RALBFv3_RS22795 at 1886.393 kb on - strand, within RALBFv3_RS22795 at 1886.494 kb on + strand, within RALBFv3_RS22800 at 1886.495 kb on - strand, within RALBFv3_RS22800 at 1886.564 kb on - strand, within RALBFv3_RS22800 at 1886.564 kb on - strand, within RALBFv3_RS22800 at 1886.630 kb on + strand, within RALBFv3_RS22800 at 1886.631 kb on - strand, within RALBFv3_RS22800 at 1886.639 kb on - strand, within RALBFv3_RS22800 at 1886.639 kb on - strand, within RALBFv3_RS22800 at 1886.639 kb on - strand, within RALBFv3_RS22800 at 1886.660 kb on + strand, within RALBFv3_RS22800 at 1886.661 kb on - strand, within RALBFv3_RS22800 at 1886.701 kb on + strand at 1886.702 kb on - strand at 1886.960 kb on + strand, within RALBFv3_RS22805 at 1886.960 kb on + strand, within RALBFv3_RS22805 at 1886.961 kb on - strand, within RALBFv3_RS22805 at 1887.256 kb on + strand, within RALBFv3_RS22810 at 1887.257 kb on - strand, within RALBFv3_RS22810 at 1887.298 kb on + strand, within RALBFv3_RS22810 at 1887.298 kb on + strand, within RALBFv3_RS22810 at 1887.298 kb on + strand, within RALBFv3_RS22810 at 1887.299 kb on - strand, within RALBFv3_RS22810 at 1887.299 kb on - strand, within RALBFv3_RS22810 at 1887.300 kb on + strand, within RALBFv3_RS22810 at 1887.301 kb on + strand, within RALBFv3_RS22810 at 1887.301 kb on + strand, within RALBFv3_RS22810 at 1887.301 kb on + strand, within RALBFv3_RS22810 at 1887.301 kb on + strand, within RALBFv3_RS22810 at 1887.301 kb on + strand, within RALBFv3_RS22810 at 1887.301 kb on + strand, within RALBFv3_RS22810 at 1887.301 kb on + strand, within RALBFv3_RS22810 at 1887.302 kb on - strand, within RALBFv3_RS22810 at 1887.302 kb on - strand, within RALBFv3_RS22810 at 1887.302 kb on - strand, within RALBFv3_RS22810 at 1887.302 kb on - strand, within RALBFv3_RS22810 at 1887.302 kb on - strand, within RALBFv3_RS22810 at 1887.326 kb on + strand, within RALBFv3_RS22810 at 1887.327 kb on - strand, within RALBFv3_RS22810 at 1887.366 kb on + strand, within RALBFv3_RS22810 at 1887.366 kb on + strand, within RALBFv3_RS22810 at 1887.366 kb on + strand, within RALBFv3_RS22810 at 1887.366 kb on + strand, within RALBFv3_RS22810 at 1887.436 kb on + strand, within RALBFv3_RS22810 at 1887.436 kb on + strand, within RALBFv3_RS22810 at 1887.436 kb on + strand, within RALBFv3_RS22810 at 1887.436 kb on + strand, within RALBFv3_RS22810 at 1887.437 kb on - strand, within RALBFv3_RS22810 at 1887.514 kb on + strand at 1887.518 kb on + strand at 1887.519 kb on - strand at 1887.520 kb on + strand at 1887.521 kb on - strand at 1887.521 kb on - strand at 1887.543 kb on + strand at 1887.544 kb on - strand at 1887.544 kb on - strand at 1887.544 kb on - strand at 1887.544 kb on - strand at 1887.544 kb on - strand at 1887.544 kb on - strand at 1887.544 kb on - strand at 1887.544 kb on - strand at 1887.641 kb on + strand at 1887.849 kb on + strand, within RALBFv3_RS22815 at 1888.032 kb on + strand, within RALBFv3_RS22815 at 1888.033 kb on - strand, within RALBFv3_RS22815 at 1888.035 kb on + strand, within RALBFv3_RS22815 at 1888.035 kb on + strand, within RALBFv3_RS22815 at 1888.035 kb on + strand, within RALBFv3_RS22815 at 1888.036 kb on - strand, within RALBFv3_RS22815 at 1888.036 kb on - strand, within RALBFv3_RS22815
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,886,019 + +1.3 1,886,019 + +1.6 1,886,019 + +1.0 1,886,020 - +1.9 1,886,020 - +2.4 1,886,020 - +2.4 1,886,020 - +2.8 1,886,020 - +2.6 1,886,104 + +0.8 1,886,104 + +1.0 1,886,104 + +0.4 1,886,104 + +0.7 1,886,104 + +1.1 1,886,104 + +0.7 1,886,104 + +0.1 1,886,122 + +1.0 1,886,122 + +0.7 1,886,122 + +1.1 1,886,123 - +0.5 1,886,123 - -1.2 1,886,126 + +0.5 1,886,126 + +0.6 1,886,126 + +0.8 1,886,126 + +0.9 1,886,126 + +0.9 1,886,392 + RALBFv3_RS22795 0.81 -1.0 1,886,392 + RALBFv3_RS22795 0.81 -0.1 1,886,392 + RALBFv3_RS22795 0.81 +0.5 1,886,393 - RALBFv3_RS22795 0.81 +1.0 1,886,494 + RALBFv3_RS22800 0.32 +0.8 1,886,495 - RALBFv3_RS22800 0.32 +1.4 1,886,564 - RALBFv3_RS22800 0.55 +0.1 1,886,564 - RALBFv3_RS22800 0.55 +1.4 1,886,630 + RALBFv3_RS22800 0.76 +1.0 1,886,631 - RALBFv3_RS22800 0.76 +2.4 1,886,639 - RALBFv3_RS22800 0.79 +2.2 1,886,639 - RALBFv3_RS22800 0.79 +1.9 1,886,639 - RALBFv3_RS22800 0.79 +2.2 1,886,660 + RALBFv3_RS22800 0.86 +1.2 1,886,661 - RALBFv3_RS22800 0.86 +1.6 1,886,701 + +0.8 1,886,702 - +1.8 1,886,960 + RALBFv3_RS22805 0.34 +1.4 1,886,960 + RALBFv3_RS22805 0.34 +1.3 1,886,961 - RALBFv3_RS22805 0.34 +1.6 1,887,256 + RALBFv3_RS22810 0.28 -0.2 1,887,257 - RALBFv3_RS22810 0.29 +1.5 1,887,298 + RALBFv3_RS22810 0.41 +0.5 1,887,298 + RALBFv3_RS22810 0.41 -0.2 1,887,298 + RALBFv3_RS22810 0.41 -0.7 1,887,299 - RALBFv3_RS22810 0.42 +2.3 1,887,299 - RALBFv3_RS22810 0.42 +2.0 1,887,300 + RALBFv3_RS22810 0.42 +2.4 1,887,301 + RALBFv3_RS22810 0.42 +0.1 1,887,301 + RALBFv3_RS22810 0.42 +0.2 1,887,301 + RALBFv3_RS22810 0.42 -0.4 1,887,301 + RALBFv3_RS22810 0.42 +0.5 1,887,301 + RALBFv3_RS22810 0.42 +0.8 1,887,301 + RALBFv3_RS22810 0.42 +0.6 1,887,301 + RALBFv3_RS22810 0.42 -0.8 1,887,302 - RALBFv3_RS22810 0.42 +1.3 1,887,302 - RALBFv3_RS22810 0.42 -0.2 1,887,302 - RALBFv3_RS22810 0.42 +1.7 1,887,302 - RALBFv3_RS22810 0.42 +1.9 1,887,302 - RALBFv3_RS22810 0.42 +1.8 1,887,326 + RALBFv3_RS22810 0.50 +0.5 1,887,327 - RALBFv3_RS22810 0.50 +1.6 1,887,366 + RALBFv3_RS22810 0.62 +0.2 1,887,366 + RALBFv3_RS22810 0.62 +1.2 1,887,366 + RALBFv3_RS22810 0.62 -0.1 1,887,366 + RALBFv3_RS22810 0.62 +0.4 1,887,436 + RALBFv3_RS22810 0.83 +0.0 1,887,436 + RALBFv3_RS22810 0.83 +0.6 1,887,436 + RALBFv3_RS22810 0.83 +0.8 1,887,436 + RALBFv3_RS22810 0.83 -0.5 1,887,437 - RALBFv3_RS22810 0.83 +2.1 1,887,514 + +0.3 1,887,518 + +0.6 1,887,519 - +1.6 1,887,520 + -0.2 1,887,521 - -0.6 1,887,521 - +1.3 1,887,543 + +0.8 1,887,544 - +0.8 1,887,544 - +1.7 1,887,544 - +1.5 1,887,544 - +3.0 1,887,544 - +1.1 1,887,544 - +2.3 1,887,544 - +1.1 1,887,544 - +1.2 1,887,641 + +0.1 1,887,849 + RALBFv3_RS22815 0.16 -0.1 1,888,032 + RALBFv3_RS22815 0.25 +0.7 1,888,033 - RALBFv3_RS22815 0.25 +0.9 1,888,035 + RALBFv3_RS22815 0.25 -1.2 1,888,035 + RALBFv3_RS22815 0.25 +0.3 1,888,035 + RALBFv3_RS22815 0.25 +0.3 1,888,036 - RALBFv3_RS22815 0.25 +1.4 1,888,036 - RALBFv3_RS22815 0.25 +0.9
Or see this region's nucleotide sequence