Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS22805

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS22795 and RALBFv3_RS22800 overlap by 56 nucleotidesRALBFv3_RS22800 and RALBFv3_RS22805 are separated by 150 nucleotidesRALBFv3_RS22805 and RALBFv3_RS22810 overlap by 4 nucleotidesRALBFv3_RS22810 and RALBFv3_RS22815 are separated by 50 nucleotides RALBFv3_RS22795: RALBFv3_RS22795 - type II toxin-antitoxin system antitoxin, RelB/DinJ family, at 1,886,139 to 1,886,450 _RS22795 RALBFv3_RS22800: RALBFv3_RS22800 - type II toxin-antitoxin system YafQ family toxin, at 1,886,395 to 1,886,703 _RS22800 RALBFv3_RS22805: RALBFv3_RS22805 - transcriptional regulator, at 1,886,854 to 1,887,165 _RS22805 RALBFv3_RS22810: RALBFv3_RS22810 - hypothetical protein, at 1,887,162 to 1,887,491 _RS22810 RALBFv3_RS22815: RALBFv3_RS22815 - propionate catabolism operon regulatory protein PrpR, at 1,887,542 to 1,889,491 _RS22815 Position (kb) 1886 1887 1888Strain fitness (log2 ratio) -1 0 1 2 3at 1886.019 kb on + strandat 1886.019 kb on + strandat 1886.019 kb on + strandat 1886.020 kb on - strandat 1886.020 kb on - strandat 1886.020 kb on - strandat 1886.020 kb on - strandat 1886.020 kb on - strandat 1886.104 kb on + strandat 1886.104 kb on + strandat 1886.104 kb on + strandat 1886.104 kb on + strandat 1886.104 kb on + strandat 1886.104 kb on + strandat 1886.104 kb on + strandat 1886.122 kb on + strandat 1886.122 kb on + strandat 1886.122 kb on + strandat 1886.123 kb on - strandat 1886.123 kb on - strandat 1886.126 kb on + strandat 1886.126 kb on + strandat 1886.126 kb on + strandat 1886.126 kb on + strandat 1886.126 kb on + strandat 1886.392 kb on + strand, within RALBFv3_RS22795at 1886.392 kb on + strand, within RALBFv3_RS22795at 1886.392 kb on + strand, within RALBFv3_RS22795at 1886.393 kb on - strand, within RALBFv3_RS22795at 1886.494 kb on + strand, within RALBFv3_RS22800at 1886.495 kb on - strand, within RALBFv3_RS22800at 1886.564 kb on - strand, within RALBFv3_RS22800at 1886.564 kb on - strand, within RALBFv3_RS22800at 1886.630 kb on + strand, within RALBFv3_RS22800at 1886.631 kb on - strand, within RALBFv3_RS22800at 1886.639 kb on - strand, within RALBFv3_RS22800at 1886.639 kb on - strand, within RALBFv3_RS22800at 1886.639 kb on - strand, within RALBFv3_RS22800at 1886.660 kb on + strand, within RALBFv3_RS22800at 1886.661 kb on - strand, within RALBFv3_RS22800at 1886.701 kb on + strandat 1886.702 kb on - strandat 1886.960 kb on + strand, within RALBFv3_RS22805at 1886.960 kb on + strand, within RALBFv3_RS22805at 1886.961 kb on - strand, within RALBFv3_RS22805at 1887.256 kb on + strand, within RALBFv3_RS22810at 1887.257 kb on - strand, within RALBFv3_RS22810at 1887.298 kb on + strand, within RALBFv3_RS22810at 1887.298 kb on + strand, within RALBFv3_RS22810at 1887.298 kb on + strand, within RALBFv3_RS22810at 1887.299 kb on - strand, within RALBFv3_RS22810at 1887.299 kb on - strand, within RALBFv3_RS22810at 1887.300 kb on + strand, within RALBFv3_RS22810at 1887.301 kb on + strand, within RALBFv3_RS22810at 1887.301 kb on + strand, within RALBFv3_RS22810at 1887.301 kb on + strand, within RALBFv3_RS22810at 1887.301 kb on + strand, within RALBFv3_RS22810at 1887.301 kb on + strand, within RALBFv3_RS22810at 1887.301 kb on + strand, within RALBFv3_RS22810at 1887.301 kb on + strand, within RALBFv3_RS22810at 1887.302 kb on - strand, within RALBFv3_RS22810at 1887.302 kb on - strand, within RALBFv3_RS22810at 1887.302 kb on - strand, within RALBFv3_RS22810at 1887.302 kb on - strand, within RALBFv3_RS22810at 1887.302 kb on - strand, within RALBFv3_RS22810at 1887.326 kb on + strand, within RALBFv3_RS22810at 1887.327 kb on - strand, within RALBFv3_RS22810at 1887.366 kb on + strand, within RALBFv3_RS22810at 1887.366 kb on + strand, within RALBFv3_RS22810at 1887.366 kb on + strand, within RALBFv3_RS22810at 1887.366 kb on + strand, within RALBFv3_RS22810at 1887.436 kb on + strand, within RALBFv3_RS22810at 1887.436 kb on + strand, within RALBFv3_RS22810at 1887.436 kb on + strand, within RALBFv3_RS22810at 1887.436 kb on + strand, within RALBFv3_RS22810at 1887.437 kb on - strand, within RALBFv3_RS22810at 1887.514 kb on + strandat 1887.518 kb on + strandat 1887.519 kb on - strandat 1887.520 kb on + strandat 1887.521 kb on - strandat 1887.521 kb on - strandat 1887.543 kb on + strandat 1887.544 kb on - strandat 1887.544 kb on - strandat 1887.544 kb on - strandat 1887.544 kb on - strandat 1887.544 kb on - strandat 1887.544 kb on - strandat 1887.544 kb on - strandat 1887.544 kb on - strandat 1887.641 kb on + strandat 1887.849 kb on + strand, within RALBFv3_RS22815at 1888.032 kb on + strand, within RALBFv3_RS22815at 1888.033 kb on - strand, within RALBFv3_RS22815at 1888.035 kb on + strand, within RALBFv3_RS22815at 1888.035 kb on + strand, within RALBFv3_RS22815at 1888.035 kb on + strand, within RALBFv3_RS22815at 1888.036 kb on - strand, within RALBFv3_RS22815at 1888.036 kb on - strand, within RALBFv3_RS22815

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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1,886,019 + +1.3
1,886,019 + +1.6
1,886,019 + +1.0
1,886,020 - +1.9
1,886,020 - +2.4
1,886,020 - +2.4
1,886,020 - +2.8
1,886,020 - +2.6
1,886,104 + +0.8
1,886,104 + +1.0
1,886,104 + +0.4
1,886,104 + +0.7
1,886,104 + +1.1
1,886,104 + +0.7
1,886,104 + +0.1
1,886,122 + +1.0
1,886,122 + +0.7
1,886,122 + +1.1
1,886,123 - +0.5
1,886,123 - -1.2
1,886,126 + +0.5
1,886,126 + +0.6
1,886,126 + +0.8
1,886,126 + +0.9
1,886,126 + +0.9
1,886,392 + RALBFv3_RS22795 0.81 -1.0
1,886,392 + RALBFv3_RS22795 0.81 -0.1
1,886,392 + RALBFv3_RS22795 0.81 +0.5
1,886,393 - RALBFv3_RS22795 0.81 +1.0
1,886,494 + RALBFv3_RS22800 0.32 +0.8
1,886,495 - RALBFv3_RS22800 0.32 +1.4
1,886,564 - RALBFv3_RS22800 0.55 +0.1
1,886,564 - RALBFv3_RS22800 0.55 +1.4
1,886,630 + RALBFv3_RS22800 0.76 +1.0
1,886,631 - RALBFv3_RS22800 0.76 +2.4
1,886,639 - RALBFv3_RS22800 0.79 +2.2
1,886,639 - RALBFv3_RS22800 0.79 +1.9
1,886,639 - RALBFv3_RS22800 0.79 +2.2
1,886,660 + RALBFv3_RS22800 0.86 +1.2
1,886,661 - RALBFv3_RS22800 0.86 +1.6
1,886,701 + +0.8
1,886,702 - +1.8
1,886,960 + RALBFv3_RS22805 0.34 +1.4
1,886,960 + RALBFv3_RS22805 0.34 +1.3
1,886,961 - RALBFv3_RS22805 0.34 +1.6
1,887,256 + RALBFv3_RS22810 0.28 -0.2
1,887,257 - RALBFv3_RS22810 0.29 +1.5
1,887,298 + RALBFv3_RS22810 0.41 +0.5
1,887,298 + RALBFv3_RS22810 0.41 -0.2
1,887,298 + RALBFv3_RS22810 0.41 -0.7
1,887,299 - RALBFv3_RS22810 0.42 +2.3
1,887,299 - RALBFv3_RS22810 0.42 +2.0
1,887,300 + RALBFv3_RS22810 0.42 +2.4
1,887,301 + RALBFv3_RS22810 0.42 +0.1
1,887,301 + RALBFv3_RS22810 0.42 +0.2
1,887,301 + RALBFv3_RS22810 0.42 -0.4
1,887,301 + RALBFv3_RS22810 0.42 +0.5
1,887,301 + RALBFv3_RS22810 0.42 +0.8
1,887,301 + RALBFv3_RS22810 0.42 +0.6
1,887,301 + RALBFv3_RS22810 0.42 -0.8
1,887,302 - RALBFv3_RS22810 0.42 +1.3
1,887,302 - RALBFv3_RS22810 0.42 -0.2
1,887,302 - RALBFv3_RS22810 0.42 +1.7
1,887,302 - RALBFv3_RS22810 0.42 +1.9
1,887,302 - RALBFv3_RS22810 0.42 +1.8
1,887,326 + RALBFv3_RS22810 0.50 +0.5
1,887,327 - RALBFv3_RS22810 0.50 +1.6
1,887,366 + RALBFv3_RS22810 0.62 +0.2
1,887,366 + RALBFv3_RS22810 0.62 +1.2
1,887,366 + RALBFv3_RS22810 0.62 -0.1
1,887,366 + RALBFv3_RS22810 0.62 +0.4
1,887,436 + RALBFv3_RS22810 0.83 +0.0
1,887,436 + RALBFv3_RS22810 0.83 +0.6
1,887,436 + RALBFv3_RS22810 0.83 +0.8
1,887,436 + RALBFv3_RS22810 0.83 -0.5
1,887,437 - RALBFv3_RS22810 0.83 +2.1
1,887,514 + +0.3
1,887,518 + +0.6
1,887,519 - +1.6
1,887,520 + -0.2
1,887,521 - -0.6
1,887,521 - +1.3
1,887,543 + +0.8
1,887,544 - +0.8
1,887,544 - +1.7
1,887,544 - +1.5
1,887,544 - +3.0
1,887,544 - +1.1
1,887,544 - +2.3
1,887,544 - +1.1
1,887,544 - +1.2
1,887,641 + +0.1
1,887,849 + RALBFv3_RS22815 0.16 -0.1
1,888,032 + RALBFv3_RS22815 0.25 +0.7
1,888,033 - RALBFv3_RS22815 0.25 +0.9
1,888,035 + RALBFv3_RS22815 0.25 -1.2
1,888,035 + RALBFv3_RS22815 0.25 +0.3
1,888,035 + RALBFv3_RS22815 0.25 +0.3
1,888,036 - RALBFv3_RS22815 0.25 +1.4
1,888,036 - RALBFv3_RS22815 0.25 +0.9

Or see this region's nucleotide sequence