Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS12790 and RALBFv3_RS12795 are separated by 536 nucleotides RALBFv3_RS12795 and RALBFv3_RS12800 are separated by 76 nucleotides
RALBFv3_RS12790: RALBFv3_RS12790 - 4-hydroxy-3-polyprenylbenzoate decarboxylase, at 2,813,932 to 2,815,443
_RS12790
RALBFv3_RS12795: RALBFv3_RS12795 - lytic transglycosylase, at 2,815,980 to 2,817,197
_RS12795
RALBFv3_RS12800: RALBFv3_RS12800 - pyridoxal phosphate-dependent aminotransferase, at 2,817,274 to 2,818,458
_RS12800
Position (kb)
2815
2816
2817
2818 Strain fitness (log2 ratio)
-1
0
1 at 2815.556 kb on + strand at 2815.557 kb on - strand at 2815.691 kb on - strand at 2815.691 kb on - strand at 2815.741 kb on - strand at 2815.896 kb on + strand at 2815.896 kb on + strand at 2815.899 kb on + strand at 2815.899 kb on + strand at 2815.976 kb on - strand at 2815.979 kb on + strand at 2815.979 kb on + strand at 2815.980 kb on - strand at 2815.980 kb on - strand at 2815.980 kb on - strand at 2816.080 kb on - strand at 2816.590 kb on - strand, within RALBFv3_RS12795 at 2816.649 kb on + strand, within RALBFv3_RS12795 at 2816.789 kb on + strand, within RALBFv3_RS12795 at 2816.794 kb on - strand, within RALBFv3_RS12795 at 2817.338 kb on + strand at 2817.339 kb on - strand at 2817.354 kb on - strand at 2817.354 kb on - strand at 2817.483 kb on - strand, within RALBFv3_RS12800 at 2817.488 kb on + strand, within RALBFv3_RS12800 at 2817.488 kb on + strand, within RALBFv3_RS12800 at 2817.489 kb on - strand, within RALBFv3_RS12800 at 2817.590 kb on + strand, within RALBFv3_RS12800 at 2817.591 kb on - strand, within RALBFv3_RS12800 at 2817.759 kb on + strand, within RALBFv3_RS12800 at 2817.943 kb on + strand, within RALBFv3_RS12800
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 2,815,556 + -0.5 2,815,557 - +0.4 2,815,691 - +0.9 2,815,691 - +0.3 2,815,741 - +0.6 2,815,896 + -0.7 2,815,896 + -0.3 2,815,899 + -0.6 2,815,899 + -0.9 2,815,976 - -0.1 2,815,979 + -0.2 2,815,979 + +0.3 2,815,980 - +0.5 2,815,980 - -0.1 2,815,980 - -0.1 2,816,080 - +0.3 2,816,590 - RALBFv3_RS12795 0.50 +0.3 2,816,649 + RALBFv3_RS12795 0.55 +0.4 2,816,789 + RALBFv3_RS12795 0.66 -0.1 2,816,794 - RALBFv3_RS12795 0.67 +0.5 2,817,338 + -0.2 2,817,339 - -0.2 2,817,354 - -0.5 2,817,354 - -0.6 2,817,483 - RALBFv3_RS12800 0.18 +0.5 2,817,488 + RALBFv3_RS12800 0.18 -0.6 2,817,488 + RALBFv3_RS12800 0.18 -0.5 2,817,489 - RALBFv3_RS12800 0.18 +0.1 2,817,590 + RALBFv3_RS12800 0.27 -0.4 2,817,591 - RALBFv3_RS12800 0.27 -0.3 2,817,759 + RALBFv3_RS12800 0.41 -0.7 2,817,943 + RALBFv3_RS12800 0.56 +0.3
Or see this region's nucleotide sequence