Strain Fitness in Ralstonia solanacearum IBSBF1503 around RALBFv3_RS12635

Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRALBFv3_RS12630 and RALBFv3_RS12635 overlap by 14 nucleotidesRALBFv3_RS12635 and RALBFv3_RS12640 are separated by 15 nucleotides RALBFv3_RS12630: RALBFv3_RS12630 - dihydroorotase, at 2,783,758 to 2,785,035 _RS12630 RALBFv3_RS12635: RALBFv3_RS12635 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 2,785,022 to 2,785,822 _RS12635 RALBFv3_RS12640: RALBFv3_RS12640 - symmetrical bis(5'-nucleosyl)-tetraphosphatase, at 2,785,838 to 2,786,689 _RS12640 Position (kb) 2785 2786Strain fitness (log2 ratio) -3 -2 -1 0 1at 2785.021 kb on + strandat 2785.022 kb on - strandat 2785.022 kb on - strandat 2785.655 kb on + strand, within RALBFv3_RS12635at 2785.666 kb on + strand, within RALBFv3_RS12635at 2785.666 kb on + strand, within RALBFv3_RS12635at 2785.666 kb on + strand, within RALBFv3_RS12635at 2785.666 kb on + strand, within RALBFv3_RS12635at 2785.666 kb on + strand, within RALBFv3_RS12635at 2785.666 kb on + strand, within RALBFv3_RS12635at 2785.667 kb on - strand, within RALBFv3_RS12635at 2785.839 kb on + strandat 2786.066 kb on - strand, within RALBFv3_RS12640at 2786.743 kb on + strandat 2786.744 kb on - strandat 2786.744 kb on - strandat 2786.744 kb on - strandat 2786.784 kb on - strandat 2786.784 kb on - strandat 2786.803 kb on + strandat 2786.804 kb on - strandat 2786.804 kb on - strandat 2786.819 kb on + strandat 2786.819 kb on + strandat 2786.819 kb on + strandat 2786.820 kb on - strandat 2786.820 kb on - strandat 2786.820 kb on - strandat 2786.820 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
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2,785,021 + -0.3
2,785,022 - -0.0
2,785,022 - +1.0
2,785,655 + RALBFv3_RS12635 0.79 +0.5
2,785,666 + RALBFv3_RS12635 0.80 +1.1
2,785,666 + RALBFv3_RS12635 0.80 -0.2
2,785,666 + RALBFv3_RS12635 0.80 +0.0
2,785,666 + RALBFv3_RS12635 0.80 +1.1
2,785,666 + RALBFv3_RS12635 0.80 +1.0
2,785,666 + RALBFv3_RS12635 0.80 +1.0
2,785,667 - RALBFv3_RS12635 0.81 -0.4
2,785,839 + -0.5
2,786,066 - RALBFv3_RS12640 0.27 -1.3
2,786,743 + +0.0
2,786,744 - -0.4
2,786,744 - -0.2
2,786,744 - -1.3
2,786,784 - -1.5
2,786,784 - -1.5
2,786,803 + -0.4
2,786,804 - -3.2
2,786,804 - -1.0
2,786,819 + -1.9
2,786,819 + -1.1
2,786,819 + -0.4
2,786,820 - -2.2
2,786,820 - -1.6
2,786,820 - +0.0
2,786,820 - -2.3

Or see this region's nucleotide sequence