Experiment: SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RALBFv3_RS04670 and RALBFv3_RS04675 are separated by 66 nucleotides RALBFv3_RS04675 and RALBFv3_RS04680 are separated by 27 nucleotides RALBFv3_RS04680 and RALBFv3_RS04685 are separated by 13 nucleotides
RALBFv3_RS04670: RALBFv3_RS04670 - N-acetyltransferase, at 1,044,689 to 1,045,165
_RS04670
RALBFv3_RS04675: RALBFv3_RS04675 - ABC transporter ATP-binding protein, at 1,045,232 to 1,045,948
_RS04675
RALBFv3_RS04680: RALBFv3_RS04680 - ABC transporter ATP-binding protein, at 1,045,976 to 1,046,752
_RS04680
RALBFv3_RS04685: RALBFv3_RS04685 - ABC transporter ATP-binding protein, at 1,046,766 to 1,047,902
_RS04685
Position (kb)
1045
1046
1047 Strain fitness (log2 ratio)
-1
0
1
2 at 1045.093 kb on + strand, within RALBFv3_RS04670 at 1045.233 kb on + strand at 1045.245 kb on + strand at 1045.726 kb on - strand, within RALBFv3_RS04675 at 1045.759 kb on - strand, within RALBFv3_RS04675 at 1045.759 kb on - strand, within RALBFv3_RS04675 at 1045.906 kb on - strand at 1045.906 kb on - strand at 1045.934 kb on + strand at 1045.998 kb on + strand at 1045.998 kb on + strand at 1045.998 kb on + strand at 1045.998 kb on + strand at 1046.301 kb on + strand, within RALBFv3_RS04680 at 1046.302 kb on - strand, within RALBFv3_RS04680 at 1046.302 kb on - strand, within RALBFv3_RS04680 at 1046.310 kb on + strand, within RALBFv3_RS04680 at 1046.328 kb on + strand, within RALBFv3_RS04680 at 1046.329 kb on - strand, within RALBFv3_RS04680 at 1046.329 kb on - strand, within RALBFv3_RS04680 at 1046.329 kb on - strand, within RALBFv3_RS04680 at 1046.542 kb on - strand, within RALBFv3_RS04680 at 1046.583 kb on + strand, within RALBFv3_RS04680 at 1046.647 kb on - strand, within RALBFv3_RS04680 at 1046.647 kb on - strand, within RALBFv3_RS04680 at 1047.049 kb on + strand, within RALBFv3_RS04685 at 1047.050 kb on - strand, within RALBFv3_RS04685 at 1047.298 kb on + strand, within RALBFv3_RS04685 at 1047.299 kb on - strand, within RALBFv3_RS04685 at 1047.299 kb on - strand, within RALBFv3_RS04685 at 1047.301 kb on + strand, within RALBFv3_RS04685 at 1047.302 kb on - strand, within RALBFv3_RS04685 at 1047.302 kb on - strand, within RALBFv3_RS04685 at 1047.302 kb on - strand, within RALBFv3_RS04685 at 1047.304 kb on + strand, within RALBFv3_RS04685 at 1047.304 kb on + strand, within RALBFv3_RS04685 at 1047.304 kb on + strand, within RALBFv3_RS04685 at 1047.304 kb on + strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.305 kb on - strand, within RALBFv3_RS04685 at 1047.664 kb on + strand, within RALBFv3_RS04685 at 1047.664 kb on + strand, within RALBFv3_RS04685 at 1047.665 kb on - strand, within RALBFv3_RS04685 at 1047.691 kb on + strand, within RALBFv3_RS04685 at 1047.692 kb on - strand, within RALBFv3_RS04685
Per-strain Table
Position Strand Gene LocusTag Fraction SMSA with Ralstonia_antibiotic_mix_minus_Triphenyltetrazoliumchloride remove 1,045,093 + RALBFv3_RS04670 0.85 +2.1 1,045,233 + -0.4 1,045,245 + +1.1 1,045,726 - RALBFv3_RS04675 0.69 +1.1 1,045,759 - RALBFv3_RS04675 0.74 +0.3 1,045,759 - RALBFv3_RS04675 0.74 +0.2 1,045,906 - +1.1 1,045,906 - +0.5 1,045,934 + +0.4 1,045,998 + -0.4 1,045,998 + +0.2 1,045,998 + -0.5 1,045,998 + -0.5 1,046,301 + RALBFv3_RS04680 0.42 -1.1 1,046,302 - RALBFv3_RS04680 0.42 +0.6 1,046,302 - RALBFv3_RS04680 0.42 +0.3 1,046,310 + RALBFv3_RS04680 0.43 +0.5 1,046,328 + RALBFv3_RS04680 0.45 +0.6 1,046,329 - RALBFv3_RS04680 0.45 -0.5 1,046,329 - RALBFv3_RS04680 0.45 +0.8 1,046,329 - RALBFv3_RS04680 0.45 +0.9 1,046,542 - RALBFv3_RS04680 0.73 +0.4 1,046,583 + RALBFv3_RS04680 0.78 +0.2 1,046,647 - RALBFv3_RS04680 0.86 +1.1 1,046,647 - RALBFv3_RS04680 0.86 +0.1 1,047,049 + RALBFv3_RS04685 0.25 +0.1 1,047,050 - RALBFv3_RS04685 0.25 -0.3 1,047,298 + RALBFv3_RS04685 0.47 +1.1 1,047,299 - RALBFv3_RS04685 0.47 -0.0 1,047,299 - RALBFv3_RS04685 0.47 +0.3 1,047,301 + RALBFv3_RS04685 0.47 +1.1 1,047,302 - RALBFv3_RS04685 0.47 +1.1 1,047,302 - RALBFv3_RS04685 0.47 -0.8 1,047,302 - RALBFv3_RS04685 0.47 +0.4 1,047,304 + RALBFv3_RS04685 0.47 +0.5 1,047,304 + RALBFv3_RS04685 0.47 -0.1 1,047,304 + RALBFv3_RS04685 0.47 +1.1 1,047,304 + RALBFv3_RS04685 0.47 +1.1 1,047,305 - RALBFv3_RS04685 0.47 -0.2 1,047,305 - RALBFv3_RS04685 0.47 +0.9 1,047,305 - RALBFv3_RS04685 0.47 -0.0 1,047,305 - RALBFv3_RS04685 0.47 -0.0 1,047,305 - RALBFv3_RS04685 0.47 +0.5 1,047,305 - RALBFv3_RS04685 0.47 -0.3 1,047,305 - RALBFv3_RS04685 0.47 -0.1 1,047,305 - RALBFv3_RS04685 0.47 -0.1 1,047,305 - RALBFv3_RS04685 0.47 +0.4 1,047,305 - RALBFv3_RS04685 0.47 +0.1 1,047,664 + RALBFv3_RS04685 0.79 +0.3 1,047,664 + RALBFv3_RS04685 0.79 -0.0 1,047,665 - RALBFv3_RS04685 0.79 +0.4 1,047,691 + RALBFv3_RS04685 0.81 -0.4 1,047,692 - RALBFv3_RS04685 0.81 +0.2
Or see this region's nucleotide sequence