Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt panC and panB are separated by 76 nucleotides panB and folK-1 are separated by 56 nucleotides folK-1 and pcnB overlap by 1 nucleotides
SO0869: panC - pantoate--beta-alanine ligase (NCBI ptt file), at 899,247 to 900,092
panC
SO0870: panB - 3-methyl-2-oxobutanoate hydroxymethyltransferase (NCBI ptt file), at 900,169 to 900,963
panB
SO0871: folK-1 - 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase (NCBI ptt file), at 901,020 to 901,508
folK-1
SO0872: pcnB - polyA polymerase (NCBI ptt file), at 901,508 to 902,824
pcnB
Position (kb) 901
902 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 900.166 kb on - strand at 900.357 kb on - strand, within panB at 900.551 kb on - strand, within panB at 900.740 kb on - strand, within panB at 900.800 kb on - strand, within panB at 900.947 kb on - strand at 900.962 kb on - strand at 901.040 kb on - strand at 901.074 kb on + strand, within folK-1 at 901.571 kb on - strand at 901.752 kb on - strand, within pcnB at 902.032 kb on - strand, within pcnB at 902.288 kb on - strand, within pcnB at 902.288 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.343 kb on - strand, within pcnB at 902.358 kb on - strand, within pcnB
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 900,166 - +1.4 900,357 - panB SO0870 0.24 -0.8 900,551 - panB SO0870 0.48 -3.8 900,740 - panB SO0870 0.72 -0.8 900,800 - panB SO0870 0.79 +2.4 900,947 - +4.0 900,962 - +2.1 901,040 - -0.7 901,074 + folK-1 SO0871 0.11 -0.8 901,571 - -2.0 901,752 - pcnB SO0872 0.19 -1.3 902,032 - pcnB SO0872 0.40 -2.1 902,288 - pcnB SO0872 0.59 +1.4 902,288 - pcnB SO0872 0.59 -1.3 902,343 - pcnB SO0872 0.63 -0.7 902,343 - pcnB SO0872 0.63 -0.8 902,343 - pcnB SO0872 0.63 -0.2 902,343 - pcnB SO0872 0.63 +0.4 902,343 - pcnB SO0872 0.63 +2.4 902,343 - pcnB SO0872 0.63 +0.4 902,343 - pcnB SO0872 0.63 -0.6 902,343 - pcnB SO0872 0.63 +0.8 902,343 - pcnB SO0872 0.63 +0.8 902,343 - pcnB SO0872 0.63 -0.6 902,358 - pcnB SO0872 0.65 -0.2
Or see this region's nucleotide sequence