Experiment: Dictyostellum sp.mixed culture-1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt miaA and hfq are separated by 91 nucleotides hfq and hflX are separated by 23 nucleotides hflX and hflK are separated by 36 nucleotides
SO0602: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI ptt file), at 628,738 to 629,664
miaA
SO0603: hfq - host factor-I protein (NCBI ptt file), at 629,756 to 630,028
hfq
SO0604: hflX - GTP-binding protein HflX (NCBI ptt file), at 630,052 to 631,359
hflX
SO0605: hflK - hflK protein (NCBI ptt file), at 631,396 to 632,541
hflK
Position (kb) 630
631
632 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 629.302 kb on + strand, within miaA at 629.395 kb on + strand, within miaA at 629.471 kb on + strand, within miaA at 629.942 kb on + strand, within hfq at 629.999 kb on + strand, within hfq at 630.310 kb on + strand, within hflX at 630.354 kb on + strand, within hflX at 630.359 kb on + strand, within hflX at 630.402 kb on - strand, within hflX at 630.502 kb on + strand, within hflX at 630.697 kb on + strand, within hflX at 630.697 kb on + strand, within hflX at 630.697 kb on + strand, within hflX at 630.774 kb on + strand, within hflX at 630.803 kb on + strand, within hflX at 631.050 kb on + strand, within hflX at 631.050 kb on + strand, within hflX at 631.056 kb on + strand, within hflX at 631.056 kb on + strand, within hflX at 631.080 kb on + strand, within hflX at 631.247 kb on + strand at 631.299 kb on + strand at 631.311 kb on + strand at 631.389 kb on + strand at 631.550 kb on + strand, within hflK at 631.550 kb on + strand, within hflK at 632.028 kb on + strand, within hflK at 632.119 kb on + strand, within hflK at 632.247 kb on + strand, within hflK at 632.254 kb on + strand, within hflK at 632.259 kb on + strand, within hflK at 632.316 kb on + strand, within hflK
Per-strain Table
Position Strand Gene LocusTag Fraction Dictyostellum sp.mixed culture-1 remove 629,302 + miaA SO0602 0.61 +1.3 629,395 + miaA SO0602 0.71 +1.3 629,471 + miaA SO0602 0.79 +0.7 629,942 + hfq SO0603 0.68 +1.3 629,999 + hfq SO0603 0.89 +2.3 630,310 + hflX SO0604 0.20 -1.3 630,354 + hflX SO0604 0.23 -3.2 630,359 + hflX SO0604 0.23 -0.7 630,402 - hflX SO0604 0.27 +2.3 630,502 + hflX SO0604 0.34 -0.5 630,697 + hflX SO0604 0.49 +0.7 630,697 + hflX SO0604 0.49 -0.3 630,697 + hflX SO0604 0.49 -0.8 630,774 + hflX SO0604 0.55 -3.4 630,803 + hflX SO0604 0.57 -1.6 631,050 + hflX SO0604 0.76 +0.7 631,050 + hflX SO0604 0.76 -1.9 631,056 + hflX SO0604 0.77 -1.8 631,056 + hflX SO0604 0.77 -2.4 631,080 + hflX SO0604 0.79 -2.9 631,247 + -3.0 631,299 + -2.0 631,311 + -2.7 631,389 + -2.6 631,550 + hflK SO0605 0.13 -0.7 631,550 + hflK SO0605 0.13 +0.7 632,028 + hflK SO0605 0.55 -1.9 632,119 + hflK SO0605 0.63 -2.1 632,247 + hflK SO0605 0.74 -0.7 632,254 + hflK SO0605 0.75 -1.4 632,259 + hflK SO0605 0.75 +0.2 632,316 + hflK SO0605 0.80 -0.0
Or see this region's nucleotide sequence